Table of Content
I.Project Summary
II.Workflow Checklist
III.NGS Sequencing Results
IV.Complete Report Download
V.Raw Sequence Data Download
VI.Analysis - DADA2 Read Processing
 Sample Meta Info
 Read Count by Sample
VII.Analysis - Read Taxonomy Assignment
 Taxonomy Barplots
VIII.Analysis - Alpha Diversity
IX.Analysis - Beta Diversity
X.Analysis - Differential Abundance
 ANCOM Result
 LEfSe Result
XI.Analysis - Heatmap Profile
XII.Analysis - Network Association
XIII.Disclaimer

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FOMC Service Report

16S rRNA Gene V1V3 Amplicon Sequencing

Version V1.50

Version History

The Forsyth Institute, Cambridge, MA, USA
June 24, 2025

Project ID: FOMC20139_Sanofi_2

I. Project Summary

Project FOMC20139_Sanofi_2 services include NGS sequencing of the V1V3 region of the 16S rRNA gene amplicons from the samples. First and foremost, please download this report, as well as the sequence raw data from the download links provided below. These links will expire after 60 days. We cannot guarantee the availability of your data after 60 days.

Full Bioinformatics analysis service was requested. We provide many analyses, starting from the raw sequence quality and noise filtering, pair reads merging, as well as chimera filtering for the sequences, using the DADA2 denosing algorithm and pipeline.

We also provide many downstream analyses such as taxonomy assignment, alpha and beta diversity analyses, and differential abundance analysis.

For taxonomy assignment, most informative would be the taxonomy barplots. We provide an interactive barplots to show the relative abundance of microbes at different taxonomy levels (from Phylum to species) that you can choose.

If you specify which groups of samples you want to compare for differential abundance, we provide both ANCOM and LEfSe differential abundance analysis.

 

II. Workflow Checklist

☑1.Sample Received
☑2.Sample Quality Evaluated
☑3.Sample Prepared for Sequencing
☑4.Next-Gen Sequencing
☑5.Sequence Quality Check
☑6.Absolute Abundance
☑7.Report and Raw Sequence Data Available for Download
☑8.Bioinformatics Analysis - Reads Processing (DADA2 Quality Trimming, Denoising, Paired Reads Merging)
☑9.Bioinformatics Analysis - Reads Taxonomy Assignment
☑10.Bioinformatics Analysis - Alpha Diversity Analysis
☑11.Bioinformatics Analysis - Beta Diversity Analysis
☑12.Bioinformatics Analysis - Differential Abundance Analysis
☑13.Bioinformatics Analysis - Heatmap Profile
☑14.Bioinformatics Analysis - Network Association
 

III. NGS Sequencing

The samples were processed and analyzed with the ZymoBIOMICS® Service: Targeted Metagenomic Sequencing (Zymo Research, Irvine, CA).

DNA Extraction: If DNA extraction was performed, the following DNA extraction kit was used according to the manufacturer’s instructions:

☑ZymoBIOMICS®-96 MagBead DNA Kit (Zymo Research, Irvine, CA)
☐N/A (DNA Extraction Not Performed)
Elution Volume: 50µL
Additional Notes: NA

Targeted Library Preparation: The DNA samples were prepared for targeted sequencing with the Quick-16S™ NGS Library Prep Kit (Zymo Research, Irvine, CA). These primers were custom designed by Zymo Research to provide the best coverage of the 16S gene while maintaining high sensitivity. The primer sets used in this project are marked below:

☐Quick-16S™ Primer Set V1-V2 (Zymo Research, Irvine, CA)
☑Quick-16S™ Primer Set V1-V3 (Zymo Research, Irvine, CA)
☐Quick-16S™ Primer Set V3-V4 (Zymo Research, Irvine, CA)
☐Quick-16S™ Primer Set V4 (Zymo Research, Irvine, CA)
☐Quick-16S™ Primer Set V6-V8 (Zymo Research, Irvine, CA)
Additional Notes: NA

The sequencing library was prepared using an innovative library preparation process in which PCR reactions were performed in real-time PCR machines to control cycles and therefore limit PCR chimera formation. The final PCR products were quantified with qPCR fluorescence readings and pooled together based on equal molarity. The final pooled library was cleaned up with the Select-a-Size DNA Clean & Concentrator™ (Zymo Research, Irvine, CA), then quantified with TapeStation® (Agilent Technologies, Santa Clara, CA) and Qubit® (Thermo Fisher Scientific, Waltham, WA).

Control Samples: The ZymoBIOMICS® Microbial Community Standard (Zymo Research, Irvine, CA) was used as a positive control for each DNA extraction, if performed. The ZymoBIOMICS® Microbial Community DNA Standard (Zymo Research, Irvine, CA) was used as a positive control for each targeted library preparation. Negative controls (i.e. blank extraction control, blank library preparation control) were included to assess the level of bioburden carried by the wet-lab process.

Sequencing: The final library was sequenced on Illumina® NextSeq 2000™ with a p1 (Illumina, Sand Diego, CA) reagent kit (600 cycles). The sequencing was performed with 25% PhiX spike-in.

Absolute Abundance Quantification*: A quantitative real-time PCR was set up with a standard curve. The standard curve was made with plasmid DNA containing one copy of the 16S gene and one copy of the fungal ITS2 region prepared in 10-fold serial dilutions. The primers used were the same as those used in Targeted Library Preparation. The equation generated by the plasmid DNA standard curve was used to calculate the number of gene copies in the reaction for each sample. The PCR input volume (2 µl) was used to calculate the number of gene copies per microliter in each DNA sample.
The number of genome copies per microliter DNA sample was calculated by dividing the gene copy number by an assumed number of gene copies per genome. The value used for 16S copies per genome is 4. The value used for ITS copies per genome is 200. The amount of DNA per microliter DNA sample was calculated using an assumed genome size of 4.64 x 106 bp, the genome size of Escherichia coli, for 16S samples, or an assumed genome size of 1.20 x 107 bp, the genome size of Saccharomyces cerevisiae, for ITS samples. This calculation is shown below:

Calculated Total DNA = Calculated Total Genome Copies × Assumed Genome Size (4.64 × 106 bp) ×
Average Molecular Weight of a DNA bp (660 g/mole/bp) ÷ Avogadro’s Number (6.022 x 1023/mole)


* Absolute Abundance Quantification is only available for 16S and ITS analyses.

The absolute abundance standard curve data can be viewed in Excel here:

The absolute abundance standard curve is shown below:

Absolute Abundance Standard Curve

 

IV. Complete Report Download

The complete report of your project, including all links in this report, can be downloaded by clicking the link provided below. The downloaded file is a compressed ZIP file and once unzipped, open the file “REPORT.html” (may only shown as "REPORT" in your computer) by double clicking it. Your default web browser will open it and you will see the exact content of this report.

Please download and save the file to your computer storage device. The download link will expire after 60 days upon your receiving of this report.

Complete report download link:

To view the report, please follow the following steps:

1.Download the .zip file from the report link above.
2.Extract all the contents of the downloaded .zip file to your desktop.
3.Open the extracted folder and find the "REPORT.html" (may shown as only "REPORT").
4.Open (double-clicking) the REPORT.html file. Your default browser will open the top age of the complete report. Within the report, there are links to view all the analyses performed for the project.

 

V. Raw Sequence Data Download

The raw NGS sequence data is available for download with the link provided below. The data is a compressed ZIP file and can be unzipped to individual sequence files. Since this is a pair-end sequencing, each of your samples is represented by two sequence files, one for READ 1, with the file extension “*_R1.fastq.gz”, another READ 2, with the file extension “*_R1.fastq.gz”. The files are in FASTQ format and are compressed. FASTQ format is a text-based data format for storing both a biological sequence and its corresponding quality scores. Most sequence analysis software will be able to open them. The Sample IDs associated with the R1 and R2 fastq files are listed in the table below:



Sample IDOriginal Sample IDRead 1 File NameRead 2 File Name


F20139.S100original sample ID herezr20139_100V1V3_R1.fastq.gzzr20139_100V1V3_R2.fastq.gz


F20139.S101original sample ID herezr20139_101V1V3_R1.fastq.gzzr20139_101V1V3_R2.fastq.gz


F20139.S102original sample ID herezr20139_102V1V3_R1.fastq.gzzr20139_102V1V3_R2.fastq.gz


F20139.S103original sample ID herezr20139_103V1V3_R1.fastq.gzzr20139_103V1V3_R2.fastq.gz


F20139.S104original sample ID herezr20139_104V1V3_R1.fastq.gzzr20139_104V1V3_R2.fastq.gz


F20139.S105original sample ID herezr20139_105V1V3_R1.fastq.gzzr20139_105V1V3_R2.fastq.gz


F20139.S106original sample ID herezr20139_106V1V3_R1.fastq.gzzr20139_106V1V3_R2.fastq.gz


F20139.S107original sample ID herezr20139_107V1V3_R1.fastq.gzzr20139_107V1V3_R2.fastq.gz


F20139.S108original sample ID herezr20139_108V1V3_R1.fastq.gzzr20139_108V1V3_R2.fastq.gz


F20139.S109original sample ID herezr20139_109V1V3_R1.fastq.gzzr20139_109V1V3_R2.fastq.gz


F20139.S010original sample ID herezr20139_10V1V3_R1.fastq.gzzr20139_10V1V3_R2.fastq.gz


F20139.S110original sample ID herezr20139_110V1V3_R1.fastq.gzzr20139_110V1V3_R2.fastq.gz


F20139.S111original sample ID herezr20139_111V1V3_R1.fastq.gzzr20139_111V1V3_R2.fastq.gz


F20139.S112original sample ID herezr20139_112V1V3_R1.fastq.gzzr20139_112V1V3_R2.fastq.gz


F20139.S113original sample ID herezr20139_113V1V3_R1.fastq.gzzr20139_113V1V3_R2.fastq.gz


F20139.S114original sample ID herezr20139_114V1V3_R1.fastq.gzzr20139_114V1V3_R2.fastq.gz


F20139.S115original sample ID herezr20139_115V1V3_R1.fastq.gzzr20139_115V1V3_R2.fastq.gz


F20139.S116original sample ID herezr20139_116V1V3_R1.fastq.gzzr20139_116V1V3_R2.fastq.gz


F20139.S117original sample ID herezr20139_117V1V3_R1.fastq.gzzr20139_117V1V3_R2.fastq.gz


F20139.S118original sample ID herezr20139_118V1V3_R1.fastq.gzzr20139_118V1V3_R2.fastq.gz


F20139.S119original sample ID herezr20139_119V1V3_R1.fastq.gzzr20139_119V1V3_R2.fastq.gz


F20139.S011original sample ID herezr20139_11V1V3_R1.fastq.gzzr20139_11V1V3_R2.fastq.gz


F20139.S120original sample ID herezr20139_120V1V3_R1.fastq.gzzr20139_120V1V3_R2.fastq.gz


F20139.S121original sample ID herezr20139_121V1V3_R1.fastq.gzzr20139_121V1V3_R2.fastq.gz


F20139.S122original sample ID herezr20139_122V1V3_R1.fastq.gzzr20139_122V1V3_R2.fastq.gz


F20139.S123original sample ID herezr20139_123V1V3_R1.fastq.gzzr20139_123V1V3_R2.fastq.gz


F20139.S124original sample ID herezr20139_124V1V3_R1.fastq.gzzr20139_124V1V3_R2.fastq.gz


F20139.S125original sample ID herezr20139_125V1V3_R1.fastq.gzzr20139_125V1V3_R2.fastq.gz


F20139.S126original sample ID herezr20139_126V1V3_R1.fastq.gzzr20139_126V1V3_R2.fastq.gz


F20139.S127original sample ID herezr20139_127V1V3_R1.fastq.gzzr20139_127V1V3_R2.fastq.gz


F20139.S128original sample ID herezr20139_128V1V3_R1.fastq.gzzr20139_128V1V3_R2.fastq.gz


F20139.S129original sample ID herezr20139_129V1V3_R1.fastq.gzzr20139_129V1V3_R2.fastq.gz


F20139.S012original sample ID herezr20139_12V1V3_R1.fastq.gzzr20139_12V1V3_R2.fastq.gz


F20139.S130original sample ID herezr20139_130V1V3_R1.fastq.gzzr20139_130V1V3_R2.fastq.gz


F20139.S131original sample ID herezr20139_131V1V3_R1.fastq.gzzr20139_131V1V3_R2.fastq.gz


F20139.S132original sample ID herezr20139_132V1V3_R1.fastq.gzzr20139_132V1V3_R2.fastq.gz


F20139.S133original sample ID herezr20139_133V1V3_R1.fastq.gzzr20139_133V1V3_R2.fastq.gz


F20139.S134original sample ID herezr20139_134V1V3_R1.fastq.gzzr20139_134V1V3_R2.fastq.gz


F20139.S135original sample ID herezr20139_135V1V3_R1.fastq.gzzr20139_135V1V3_R2.fastq.gz


F20139.S136original sample ID herezr20139_136V1V3_R1.fastq.gzzr20139_136V1V3_R2.fastq.gz


F20139.S137original sample ID herezr20139_137V1V3_R1.fastq.gzzr20139_137V1V3_R2.fastq.gz


F20139.S138original sample ID herezr20139_138V1V3_R1.fastq.gzzr20139_138V1V3_R2.fastq.gz


F20139.S139original sample ID herezr20139_139V1V3_R1.fastq.gzzr20139_139V1V3_R2.fastq.gz


F20139.S013original sample ID herezr20139_13V1V3_R1.fastq.gzzr20139_13V1V3_R2.fastq.gz


F20139.S140original sample ID herezr20139_140V1V3_R1.fastq.gzzr20139_140V1V3_R2.fastq.gz


F20139.S141original sample ID herezr20139_141V1V3_R1.fastq.gzzr20139_141V1V3_R2.fastq.gz


F20139.S142original sample ID herezr20139_142V1V3_R1.fastq.gzzr20139_142V1V3_R2.fastq.gz


F20139.S143original sample ID herezr20139_143V1V3_R1.fastq.gzzr20139_143V1V3_R2.fastq.gz


F20139.S144original sample ID herezr20139_144V1V3_R1.fastq.gzzr20139_144V1V3_R2.fastq.gz


F20139.S145original sample ID herezr20139_145V1V3_R1.fastq.gzzr20139_145V1V3_R2.fastq.gz


F20139.S146original sample ID herezr20139_146V1V3_R1.fastq.gzzr20139_146V1V3_R2.fastq.gz


F20139.S147original sample ID herezr20139_147V1V3_R1.fastq.gzzr20139_147V1V3_R2.fastq.gz


F20139.S148original sample ID herezr20139_148V1V3_R1.fastq.gzzr20139_148V1V3_R2.fastq.gz


F20139.S149original sample ID herezr20139_149V1V3_R1.fastq.gzzr20139_149V1V3_R2.fastq.gz


F20139.S014original sample ID herezr20139_14V1V3_R1.fastq.gzzr20139_14V1V3_R2.fastq.gz


F20139.S150original sample ID herezr20139_150V1V3_R1.fastq.gzzr20139_150V1V3_R2.fastq.gz


F20139.S151original sample ID herezr20139_151V1V3_R1.fastq.gzzr20139_151V1V3_R2.fastq.gz


F20139.S152original sample ID herezr20139_152V1V3_R1.fastq.gzzr20139_152V1V3_R2.fastq.gz


F20139.S153original sample ID herezr20139_153V1V3_R1.fastq.gzzr20139_153V1V3_R2.fastq.gz


F20139.S154original sample ID herezr20139_154V1V3_R1.fastq.gzzr20139_154V1V3_R2.fastq.gz


F20139.S155original sample ID herezr20139_155V1V3_R1.fastq.gzzr20139_155V1V3_R2.fastq.gz


F20139.S156original sample ID herezr20139_156V1V3_R1.fastq.gzzr20139_156V1V3_R2.fastq.gz


F20139.S157original sample ID herezr20139_157V1V3_R1.fastq.gzzr20139_157V1V3_R2.fastq.gz


F20139.S158original sample ID herezr20139_158V1V3_R1.fastq.gzzr20139_158V1V3_R2.fastq.gz


F20139.S159original sample ID herezr20139_159V1V3_R1.fastq.gzzr20139_159V1V3_R2.fastq.gz


F20139.S015original sample ID herezr20139_15V1V3_R1.fastq.gzzr20139_15V1V3_R2.fastq.gz


F20139.S160original sample ID herezr20139_160V1V3_R1.fastq.gzzr20139_160V1V3_R2.fastq.gz


F20139.S161original sample ID herezr20139_161V1V3_R1.fastq.gzzr20139_161V1V3_R2.fastq.gz


F20139.S162original sample ID herezr20139_162V1V3_R1.fastq.gzzr20139_162V1V3_R2.fastq.gz


F20139.S163original sample ID herezr20139_163V1V3_R1.fastq.gzzr20139_163V1V3_R2.fastq.gz


F20139.S164original sample ID herezr20139_164V1V3_R1.fastq.gzzr20139_164V1V3_R2.fastq.gz


F20139.S165original sample ID herezr20139_165V1V3_R1.fastq.gzzr20139_165V1V3_R2.fastq.gz


F20139.S166original sample ID herezr20139_166V1V3_R1.fastq.gzzr20139_166V1V3_R2.fastq.gz


F20139.S167original sample ID herezr20139_167V1V3_R1.fastq.gzzr20139_167V1V3_R2.fastq.gz


F20139.S168original sample ID herezr20139_168V1V3_R1.fastq.gzzr20139_168V1V3_R2.fastq.gz


F20139.S169original sample ID herezr20139_169V1V3_R1.fastq.gzzr20139_169V1V3_R2.fastq.gz


F20139.S016original sample ID herezr20139_16V1V3_R1.fastq.gzzr20139_16V1V3_R2.fastq.gz


F20139.S170original sample ID herezr20139_170V1V3_R1.fastq.gzzr20139_170V1V3_R2.fastq.gz


F20139.S171original sample ID herezr20139_171V1V3_R1.fastq.gzzr20139_171V1V3_R2.fastq.gz


F20139.S172original sample ID herezr20139_172V1V3_R1.fastq.gzzr20139_172V1V3_R2.fastq.gz


F20139.S173original sample ID herezr20139_173V1V3_R1.fastq.gzzr20139_173V1V3_R2.fastq.gz


F20139.S174original sample ID herezr20139_174V1V3_R1.fastq.gzzr20139_174V1V3_R2.fastq.gz


F20139.S175original sample ID herezr20139_175V1V3_R1.fastq.gzzr20139_175V1V3_R2.fastq.gz


F20139.S176original sample ID herezr20139_176V1V3_R1.fastq.gzzr20139_176V1V3_R2.fastq.gz


F20139.S177original sample ID herezr20139_177V1V3_R1.fastq.gzzr20139_177V1V3_R2.fastq.gz


F20139.S178original sample ID herezr20139_178V1V3_R1.fastq.gzzr20139_178V1V3_R2.fastq.gz


F20139.S179original sample ID herezr20139_179V1V3_R1.fastq.gzzr20139_179V1V3_R2.fastq.gz


F20139.S017original sample ID herezr20139_17V1V3_R1.fastq.gzzr20139_17V1V3_R2.fastq.gz


F20139.S180original sample ID herezr20139_180V1V3_R1.fastq.gzzr20139_180V1V3_R2.fastq.gz


F20139.S181original sample ID herezr20139_181V1V3_R1.fastq.gzzr20139_181V1V3_R2.fastq.gz


F20139.S182original sample ID herezr20139_182V1V3_R1.fastq.gzzr20139_182V1V3_R2.fastq.gz


F20139.S183original sample ID herezr20139_183V1V3_R1.fastq.gzzr20139_183V1V3_R2.fastq.gz


F20139.S184original sample ID herezr20139_184V1V3_R1.fastq.gzzr20139_184V1V3_R2.fastq.gz


F20139.S185original sample ID herezr20139_185V1V3_R1.fastq.gzzr20139_185V1V3_R2.fastq.gz


F20139.S186original sample ID herezr20139_186V1V3_R1.fastq.gzzr20139_186V1V3_R2.fastq.gz


F20139.S187original sample ID herezr20139_187V1V3_R1.fastq.gzzr20139_187V1V3_R2.fastq.gz


F20139.S188original sample ID herezr20139_188V1V3_R1.fastq.gzzr20139_188V1V3_R2.fastq.gz


F20139.S189original sample ID herezr20139_189V1V3_R1.fastq.gzzr20139_189V1V3_R2.fastq.gz


F20139.S018original sample ID herezr20139_18V1V3_R1.fastq.gzzr20139_18V1V3_R2.fastq.gz


F20139.S190original sample ID herezr20139_190V1V3_R1.fastq.gzzr20139_190V1V3_R2.fastq.gz


F20139.S191original sample ID herezr20139_191V1V3_R1.fastq.gzzr20139_191V1V3_R2.fastq.gz


F20139.S192original sample ID herezr20139_192V1V3_R1.fastq.gzzr20139_192V1V3_R2.fastq.gz


F20139.S193original sample ID herezr20139_193V1V3_R1.fastq.gzzr20139_193V1V3_R2.fastq.gz


F20139.S194original sample ID herezr20139_194V1V3_R1.fastq.gzzr20139_194V1V3_R2.fastq.gz


F20139.S195original sample ID herezr20139_195V1V3_R1.fastq.gzzr20139_195V1V3_R2.fastq.gz


F20139.S196original sample ID herezr20139_196V1V3_R1.fastq.gzzr20139_196V1V3_R2.fastq.gz


F20139.S197original sample ID herezr20139_197V1V3_R1.fastq.gzzr20139_197V1V3_R2.fastq.gz


F20139.S198original sample ID herezr20139_198V1V3_R1.fastq.gzzr20139_198V1V3_R2.fastq.gz


F20139.S199original sample ID herezr20139_199V1V3_R1.fastq.gzzr20139_199V1V3_R2.fastq.gz


F20139.S019original sample ID herezr20139_19V1V3_R1.fastq.gzzr20139_19V1V3_R2.fastq.gz


F20139.S001original sample ID herezr20139_1V1V3_R1.fastq.gzzr20139_1V1V3_R2.fastq.gz


F20139.S200original sample ID herezr20139_200V1V3_R1.fastq.gzzr20139_200V1V3_R2.fastq.gz


F20139.S201original sample ID herezr20139_201V1V3_R1.fastq.gzzr20139_201V1V3_R2.fastq.gz


F20139.S202original sample ID herezr20139_202V1V3_R1.fastq.gzzr20139_202V1V3_R2.fastq.gz


F20139.S203original sample ID herezr20139_203V1V3_R1.fastq.gzzr20139_203V1V3_R2.fastq.gz


F20139.S204original sample ID herezr20139_204V1V3_R1.fastq.gzzr20139_204V1V3_R2.fastq.gz


F20139.S205original sample ID herezr20139_205V1V3_R1.fastq.gzzr20139_205V1V3_R2.fastq.gz


F20139.S206original sample ID herezr20139_206V1V3_R1.fastq.gzzr20139_206V1V3_R2.fastq.gz


F20139.S207original sample ID herezr20139_207V1V3_R1.fastq.gzzr20139_207V1V3_R2.fastq.gz


F20139.S208original sample ID herezr20139_208V1V3_R1.fastq.gzzr20139_208V1V3_R2.fastq.gz


F20139.S209original sample ID herezr20139_209V1V3_R1.fastq.gzzr20139_209V1V3_R2.fastq.gz


F20139.S020original sample ID herezr20139_20V1V3_R1.fastq.gzzr20139_20V1V3_R2.fastq.gz


F20139.S210original sample ID herezr20139_210V1V3_R1.fastq.gzzr20139_210V1V3_R2.fastq.gz


F20139.S211original sample ID herezr20139_211V1V3_R1.fastq.gzzr20139_211V1V3_R2.fastq.gz


F20139.S212original sample ID herezr20139_212V1V3_R1.fastq.gzzr20139_212V1V3_R2.fastq.gz


F20139.S213original sample ID herezr20139_213V1V3_R1.fastq.gzzr20139_213V1V3_R2.fastq.gz


F20139.S214original sample ID herezr20139_214V1V3_R1.fastq.gzzr20139_214V1V3_R2.fastq.gz


F20139.S215original sample ID herezr20139_215V1V3_R1.fastq.gzzr20139_215V1V3_R2.fastq.gz


F20139.S216original sample ID herezr20139_216V1V3_R1.fastq.gzzr20139_216V1V3_R2.fastq.gz


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Please download and save the file to your computer storage device. The download link will expire after 60 days upon your receiving of this report.

Raw sequence data download link:

 

VI. Analysis - DADA2 Read Processing

What is DADA2?

DADA2 is a software package that models and corrects Illumina-sequenced amplicon errors [1]. DADA2 infers sample sequences exactly, without coarse-graining into OTUs, and resolves differences of as little as one nucleotide. DADA2 identified more real variants and output fewer spurious sequences than other methods.

DADA2’s advantage is that it uses more of the data. The DADA2 error model incorporates quality information, which is ignored by all other methods after filtering. The DADA2 error model incorporates quantitative abundances, whereas most other methods use abundance ranks if they use abundance at all. The DADA2 error model identifies the differences between sequences, eg. A->C, whereas other methods merely count the mismatches. DADA2 can parameterize its error model from the data itself, rather than relying on previous datasets that may or may not reflect the PCR and sequencing protocols used in your study.

DADA2 Software Package is available as an R package at : https://benjjneb.github.io/dada2/index.html

References

  1. Callahan BJ, McMurdie PJ, Rosen MJ, Han AW, Johnson AJ, Holmes SP. DADA2: High-resolution sample inference from Illumina amplicon data. Nat Methods. 2016 Jul;13(7):581-3. doi: 10.1038/nmeth.3869. Epub 2016 May 23. PMID: 27214047; PMCID: PMC4927377.

Analysis Procedures:

DADA2 pipeline includes several tools for read quality control, including quality filtering, trimming, denoising, pair merging and chimera filtering. Below are the major processing steps of DADA2:

Step 1. Read trimming based on sequence quality The quality of NGS Illumina sequences often decreases toward the end of the reads. DADA2 allows to trim off the poor quality read ends in order to improve the error model building and pair mergicing performance.

Step 2. Learn the Error Rates The DADA2 algorithm makes use of a parametric error model (err) and every amplicon dataset has a different set of error rates. The learnErrors method learns this error model from the data, by alternating estimation of the error rates and inference of sample composition until they converge on a jointly consistent solution. As in many machine-learning problems, the algorithm must begin with an initial guess, for which the maximum possible error rates in this data are used (the error rates if only the most abundant sequence is correct and all the rest are errors).

Step 3. Infer amplicon sequence variants (ASVs) based on the error model built in previous step. This step is also called sequence "denoising". The outcome of this step is a list of ASVs that are the equivalent of oligonucleotides.

Step 4. Merge paired reads. If the sequencing products are read pairs, DADA2 will merge the R1 and R2 ASVs into single sequences. Merging is performed by aligning the denoised forward reads with the reverse-complement of the corresponding denoised reverse reads, and then constructing the merged “contig” sequences. By default, merged sequences are only output if the forward and reverse reads overlap by at least 12 bases, and are identical to each other in the overlap region (but these conditions can be changed via function arguments).

Step 5. Remove chimera. The core dada method corrects substitution and indel errors, but chimeras remain. Fortunately, the accuracy of sequence variants after denoising makes identifying chimeric ASVs simpler than when dealing with fuzzy OTUs. Chimeric sequences are identified if they can be exactly reconstructed by combining a left-segment and a right-segment from two more abundant “parent” sequences. The frequency of chimeric sequences varies substantially from dataset to dataset, and depends on on factors including experimental procedures and sample complexity.

Results

1. Read Quality Plots NGS sequence analaysis starts with visualizing the quality of the sequencing. Below are the quality plots of the first sample for the R1 and R2 reads separately. In gray-scale is a heat map of the frequency of each quality score at each base position. The mean quality score at each position is shown by the green line, and the quartiles of the quality score distribution by the orange lines. The forward reads are usually of better quality. It is a common practice to trim the last few nucleotides to avoid less well-controlled errors that can arise there. The trimming affects the downstream steps including error model building, merging and chimera calling. FOMC uses an empirical approach to test many combinations of different trim length in order to achieve best final amplicon sequence variants (ASVs), see the next section “Optimal trim length for ASVs”.

Quality plots for all samples:

2. Optimal trim length for ASVs The final number of merged and chimera-filtered ASVs depends on the quality filtering (hence trimming) in the very beginning of the DADA2 pipeline. In order to achieve highest number of ASVs, an empirical approach was used -

  1. Create a random subset of each sample consisting of 5,000 R1 and 5,000 R2 (to reduce computation time)
  2. Trim 10 bases at a time from the ends of both R1 and R2 up to 50 bases
  3. For each combination of trimmed length (e.g., 300x300, 300x290, 290x290 etc), the trimmed reads are subject to the entire DADA2 pipeline for chimera-filtered merged ASVs
  4. The combination with highest percentage of the input reads becoming final ASVs is selected for the complete set of data

Below is the result of such operation, showing ASV percentages of total reads for all trimming combinations (1st Column = R1 lengths in bases; 1st Row = R2 lengths in bases):

R1/R2281271261251241231
32163.83%63.99%64.45%64.97%64.90%62.23%
31163.77%64.20%64.69%64.59%62.20%53.01%
30163.85%64.30%64.16%61.71%52.80%31.13%
29163.92%63.76%61.33%52.41%30.95%22.85%
28163.48%61.04%52.12%30.71%22.81%13.34%
27161.04%52.30%30.63%22.62%13.27%9.05%

Based on the above result, the trim length combination of R1 = 321 bases and R2 = 251 bases (highlighted red above), was chosen for generating final ASVs for all sequences. This combination generated highest number of merged non-chimeric ASVs and was used for downstream analyses, if requested.

3. Error plots from learning the error rates After DADA2 building the error model for the set of data, it is always worthwhile, as a sanity check if nothing else, to visualize the estimated error rates. The error rates for each possible transition (A→C, A→G, …) are shown below. Points are the observed error rates for each consensus quality score. The black line shows the estimated error rates after convergence of the machine-learning algorithm. The red line shows the error rates expected under the nominal definition of the Q-score. The ideal result would be the estimated error rates (black line) are a good fit to the observed rates (points), and the error rates drop with increased quality as expected.

Forward Read R1 Error Plot


Reverse Read R2 Error Plot

The PDF version of these plots are available here:

 

4. DADA2 Result Summary The table below shows the summary of the DADA2 analysis, tracking paired read counts of each samples for all the steps during DADA2 denoising process - including end-trimming (filtered), denoising (denoisedF, denoisedF), pair merging (merged) and chimera removal (nonchim).

Sample IDF20139.S001F20139.S002F20139.S003F20139.S004F20139.S005F20139.S006F20139.S007F20139.S008F20139.S009F20139.S010F20139.S011F20139.S012F20139.S013F20139.S014F20139.S015F20139.S016F20139.S017F20139.S018F20139.S019F20139.S020F20139.S021F20139.S022F20139.S023F20139.S024F20139.S025F20139.S026F20139.S027F20139.S028F20139.S029F20139.S030F20139.S031F20139.S032F20139.S033F20139.S034F20139.S035F20139.S036F20139.S037F20139.S038F20139.S039F20139.S040F20139.S041F20139.S042F20139.S043F20139.S044F20139.S045F20139.S046F20139.S047F20139.S048F20139.S049F20139.S050F20139.S051F20139.S052F20139.S053F20139.S054F20139.S055F20139.S056F20139.S057F20139.S058F20139.S059F20139.S060F20139.S061F20139.S062F20139.S063F20139.S064F20139.S065F20139.S066F20139.S067F20139.S068F20139.S069F20139.S070F20139.S071F20139.S072F20139.S073F20139.S074F20139.S075F20139.S076F20139.S077F20139.S078F20139.S079F20139.S080F20139.S081F20139.S082F20139.S083F20139.S084F20139.S085F20139.S086F20139.S087F20139.S088F20139.S089F20139.S090F20139.S091F20139.S092F20139.S093F20139.S094F20139.S095F20139.S096F20139.S097F20139.S098F20139.S099F20139.S100F20139.S101F20139.S102F20139.S103F20139.S104F20139.S105F20139.S106F20139.S107F20139.S108F20139.S109F20139.S110F20139.S111F20139.S112F20139.S113F20139.S114F20139.S115F20139.S116F20139.S117F20139.S118F20139.S119F20139.S120F20139.S121F20139.S122F20139.S123F20139.S124F20139.S125F20139.S126F20139.S127F20139.S128F20139.S129F20139.S130F20139.S131F20139.S132F20139.S133F20139.S134F20139.S135F20139.S136F20139.S137F20139.S138F20139.S139F20139.S140F20139.S141F20139.S142F20139.S143F20139.S144F20139.S145F20139.S146F20139.S147F20139.S148F20139.S149F20139.S150F20139.S151F20139.S152F20139.S153F20139.S154F20139.S155F20139.S156F20139.S157F20139.S158F20139.S159F20139.S160F20139.S161F20139.S162F20139.S163F20139.S164F20139.S165F20139.S166F20139.S167F20139.S168F20139.S169F20139.S170F20139.S171F20139.S172F20139.S173F20139.S174F20139.S175F20139.S176F20139.S177F20139.S178F20139.S179F20139.S180F20139.S181F20139.S182F20139.S183F20139.S184F20139.S185F20139.S186F20139.S187F20139.S188F20139.S189F20139.S190F20139.S191F20139.S192F20139.S193F20139.S194F20139.S195F20139.S196F20139.S197F20139.S198F20139.S199F20139.S200F20139.S201F20139.S202F20139.S203F20139.S204F20139.S205F20139.S206F20139.S207F20139.S208F20139.S209F20139.S210F20139.S211F20139.S212F20139.S213F20139.S214F20139.S215F20139.S216F20139.S217F20139.S218F20139.S219F20139.S220F20139.S221F20139.S222F20139.S223F20139.S224F20139.S225F20139.S226F20139.S227F20139.S228F20139.S229F20139.S230F20139.S231F20139.S232F20139.S233F20139.S234F20139.S235F20139.S236F20139.S237F20139.S238F20139.S239F20139.S240Row SumPercentage
input120,318203,238166,277151,812125,403149,524136,653154,527123,132140,711146,417147,238149,550140,880125,240169,884141,677141,641134,477159,754172,563177,176146,633158,543128,424187,417134,978196,880174,245141,819141,587134,691129,233152,724151,126150,626147,891145,301141,784116,562169,851167,920150,428157,993178,258180,510139,323145,523183,024171,547158,176148,905168,582189,799151,874155,423163,203154,133168,985136,476171,469191,043149,023140,374170,753151,555215,548188,951253,637186,576103,182144,637161,404148,312152,775146,863150,069112,981143,374127,437179,184229,099136,545135,536172,761141,664138,547154,628167,481148,793143,315189,863127,843146,684127,465151,787132,672126,937132,488150,919148,319134,483163,176137,723150,218151,541129,164160,714155,923141,977156,215137,224123,482147,891130,537161,383144,882164,130165,534147,823133,168141,850135,759143,145127,049145,314149,190137,578142,379138,399121,331158,928169,849143,087157,797132,867166,522134,844141,795130,218160,033153,461136,306133,444128,540158,720137,191143,240161,978152,872152,868138,237131,713165,821124,437148,213182,443141,029162,696157,573112,946130,089148,794157,509120,094144,617137,561121,237157,077147,580125,073166,905156,656134,789147,654139,359139,116158,693138,599134,402206,619160,318134,983111,338167,462148,390122,871138,590121,874172,344152,325121,453174,123211,748186,952152,559146,883123,500144,922147,74867,653102,501101,67978,419118,71398,884109,636146,886106,11397,828116,405110,86790,11696,546133,726106,327126,125156,580127,83190,055110,66191,487151,590122,127108,212138,368128,236107,899153,540132,768163,267135,089141,806150,400110,305119,550112,420120,498146,343124,50834,834,602100.00%
filtered95,241161,992132,097120,32999,869118,548108,506122,61297,700111,939115,936116,813118,174111,30299,285134,997112,488112,346106,414126,532136,548140,661116,577126,152101,890148,516106,882156,537138,251112,049112,245106,738102,610121,084119,763119,384117,665114,776112,10392,254135,043133,215118,858125,058141,619143,134111,008115,527144,889136,161125,513118,317133,566150,510120,616122,559129,990122,055134,593108,089136,373151,191117,884111,300134,680120,437171,331150,475202,083147,43481,835114,465128,139117,377121,376116,539118,69589,387113,630101,086141,816182,060107,972107,331136,516112,391110,230122,783132,676117,988113,555150,968101,233116,499100,930120,154105,790100,336105,216119,619117,719106,762129,409108,779118,776120,363102,452127,180123,459112,921123,889108,43297,861117,079103,300127,860115,038129,643131,471117,022105,645112,381107,637113,357100,947115,394118,295109,237112,668109,73196,522126,123134,664113,358125,548105,077132,522107,011112,302103,499126,755121,721107,913105,635102,073126,226108,409113,641128,402120,998121,156109,440104,352131,26698,845117,366145,253111,628128,795125,15689,492102,907118,264125,02295,024115,068109,05495,955124,661117,26199,106132,830123,978107,277116,949110,846110,260125,772109,940106,933164,171127,260107,34288,191133,093117,81097,611110,21296,550137,449121,42196,149138,798185,744164,373120,924116,26698,287115,488117,67853,57981,25780,79462,06894,13878,09087,089116,58184,01777,57792,19888,15271,51476,855106,23584,179100,145124,643101,65171,42687,67572,290120,33296,60786,082110,053101,58185,877121,889105,349129,307107,105112,372119,48187,53094,72188,98595,473115,76098,67127,665,01279.42%
denoisedF93,656160,168130,621118,78098,609117,048107,050120,95796,420110,432114,538115,397116,674110,03597,575133,361111,194111,019105,110124,614134,743138,911115,256124,663100,238146,685105,174154,829136,350110,393110,746105,300101,393119,583118,089118,026116,178113,381110,54591,060133,070131,376117,491123,220139,845141,392109,858113,956142,927134,107123,833116,614132,025148,542118,687120,843128,554120,411132,783106,092134,761149,172116,324109,715132,724118,980169,032148,431199,509145,05880,772112,667126,319115,684119,267114,840117,09188,043112,23699,665136,552180,142106,635106,002134,614110,894108,634121,103130,915116,309110,922148,785100,013115,00999,504118,622104,57098,913103,776117,642116,235105,246127,683107,373117,079118,875101,365125,340121,869111,422122,213107,03896,690115,403101,876126,316113,729127,958129,822115,195104,316110,958106,320111,84199,539113,848117,008107,691111,217108,13695,247124,240132,978111,972123,937103,740131,014105,455110,793101,980125,165120,082106,512104,140100,609124,559107,110111,700126,532119,255119,212107,919102,934129,34097,333115,775143,470110,193127,112123,43288,151101,340116,390123,32993,357113,522107,18794,348122,655115,22497,519130,120122,310105,617114,585108,899108,589123,657108,214104,927161,999124,962105,49786,824130,925116,07395,565107,84995,004135,286119,16094,649136,448184,431162,622118,889114,44396,763113,842116,00150,95577,62478,00059,43591,23974,57283,198113,54181,45975,21289,40885,33069,01773,702102,58380,88497,110121,21498,28668,78984,66469,737115,33993,26383,307106,97998,15583,122118,039100,549125,019103,142109,036115,53784,42290,96285,81591,889112,03196,26827,199,61978.08%
denoisedR92,496158,512129,018117,54697,194115,573105,783119,36994,914108,827113,089113,950115,318108,54096,082131,447109,989109,489103,791123,083133,425137,340113,811123,22099,154145,226104,018152,910134,866108,783109,604103,800100,183118,118116,252116,675114,889111,839109,31989,040131,878129,624115,869121,737137,484139,849108,444112,358140,378132,154122,602115,378130,438147,124117,196119,260127,264118,486131,280104,434133,385147,414114,597108,402130,894117,430167,299146,479197,369143,75979,413111,117124,724114,431117,647113,570115,62786,677110,79998,308137,960178,282105,023104,293132,970109,470107,730119,201129,297114,415109,710147,44198,549113,58998,045117,291103,40397,148102,261115,827115,011103,745126,204105,888115,671117,586100,204123,673120,282109,365120,379105,44795,521113,654100,211124,898112,610126,382126,953113,111103,256109,245104,611110,09797,768112,219115,652106,176109,542106,71694,052122,526131,322110,139122,135102,378129,674103,569109,46799,621123,899118,472104,431102,83198,729123,047105,840109,943125,134116,827117,798106,407101,645128,04396,050113,155142,139108,437125,475121,35686,93899,802114,988122,11791,581112,334106,30793,410121,368113,70396,548128,491121,067104,315113,232108,019107,366122,406107,086103,786160,164123,355104,20085,858129,821114,48594,648106,55593,912134,158117,67792,828134,861181,989159,999118,085113,42295,747112,699115,09850,99077,93877,60759,42689,94674,60383,597112,12980,73974,52188,70884,43568,28673,621102,26880,18996,582119,84998,28068,33983,64569,267115,89992,74482,395105,63997,15382,443117,308100,496124,212102,115106,658114,07884,17290,16584,43590,611111,46995,25726,874,22277.15%
merged88,515152,774123,869113,17592,341111,463101,241114,29190,228103,547109,231110,164109,999104,19190,776126,621106,036105,19598,818116,356128,858132,156108,923116,73794,277140,06098,611148,143126,492102,136105,57898,76995,159111,522110,193111,588110,424107,620103,62483,002127,440124,827112,161114,419128,531134,620104,428106,203132,273126,260118,806110,697124,699137,279110,513113,404121,142112,180124,32498,780128,371141,741108,968103,320124,120111,786160,565137,810186,813136,30875,827106,162119,925108,373110,214108,102111,20581,643103,81794,102131,437173,364100,87499,558125,914104,56299,901112,062121,797109,737105,739142,78593,336108,66293,700112,57695,49090,00995,275110,813111,30999,426120,282100,561109,739113,09396,627117,725112,025104,193115,874101,06291,278107,29494,147120,130105,086121,385120,532107,58699,512104,408100,256104,74591,354107,615109,27198,512104,930102,64390,290116,937125,278105,631114,74498,414122,69595,447104,60494,126118,791113,23699,29397,82191,349117,03999,900103,440115,231110,055111,937101,39997,137121,95490,391108,193133,564102,466116,481115,73283,33594,754110,105116,66485,270107,792102,51389,390114,445107,23092,212121,419115,13499,277106,732103,768102,736117,181101,27998,632154,447116,87598,48382,614123,603109,28889,167100,15689,650129,199111,77788,077128,087174,397151,990113,953108,44891,001106,568109,73443,77867,68769,62352,63179,89864,46472,270102,63772,53967,68779,88276,00761,35965,48391,81369,35887,246108,67788,69461,19474,61462,82999,89982,40974,80196,14186,55974,420105,64885,809111,26890,46695,708100,48875,48078,21774,11778,761100,87788,02225,365,46972.82%
nonchim83,044145,421117,109108,07388,074103,47396,043107,84685,823100,839104,761105,712104,288100,43787,052122,667103,19199,57895,581113,434124,559123,491105,310110,47791,220135,48993,129142,174118,46299,454100,46791,16691,046105,408104,229107,299103,971104,01999,59679,845116,828118,207108,522109,120125,041130,54699,404102,209123,412120,126111,977105,773121,453131,165105,909108,801114,135107,857119,06495,142123,034137,787106,44897,840119,438106,387154,517129,099177,612131,94570,995102,183114,110102,214106,322101,991108,00077,64898,03291,905117,214168,18095,88495,414122,720100,19594,102106,385115,855107,940101,406137,71186,330105,33190,997109,42588,51685,65190,282108,306105,73493,924113,06894,975105,530109,39389,640110,539106,066100,812110,55295,91686,470102,55987,941115,31692,607118,097112,884104,82093,602100,38097,21599,58985,921101,076104,80792,637100,20697,93787,311108,181118,966100,151110,91495,531118,64689,932101,27190,666110,787109,54394,65992,70486,946113,17995,98798,696108,958105,487102,32596,77994,560113,47587,042101,174126,50098,866110,142110,26978,94887,802103,391110,56581,576102,26994,76881,996107,551103,97887,357115,511110,03191,927103,14794,91795,281111,95894,76589,142148,697112,48493,39880,151119,261105,27983,32196,46886,452122,780103,66685,618118,370149,546141,664108,384102,79385,740101,53794,03541,39562,43167,25150,76574,82660,40766,38399,41268,24964,59277,03772,79459,75061,83787,23662,99582,691105,85678,56358,77672,31560,44891,68978,32569,96391,08481,87570,615101,87080,077106,17286,85890,86094,86673,01871,64170,15274,33896,50786,40924,112,01169.22%

This table can be downloaded as an Excel table below:

 

5. DADA2 Amplicon Sequence Variants (ASVs). A total of 22229 unique merged and chimera-free ASV sequences were identified, and their corresponding read counts for each sample are available in the "ASV Read Count Table" with rows for the ASV sequences and columns for sample. This read count table can be used for microbial profile comparison among different samples and the sequences provided in the table can be used to taxonomy assignment.

 

The table can be downloaded from this link:

 
 

Sample Meta Information

Download Sample Meta Information
#SampleIDSampleNameSampleName1NameSourceDayGroupNameGroupComparisonsBoxBox_Location
F20139.S0011B51021_Gp1_D01B51021Dental Plaque (101)_NHPPlaqueD0Gp1Plaque_Gp1Plaque_D0_Gp1_Site12Box_1Box_1_D0
F20139.S002DV385_Gp1_D0DV385Dental Plaque (101)_NHPPlaqueD0Gp1Plaque_Gp1Plaque_D0_Gp1_Site12Box_1Box_1_D0
F20139.S0031A69520_Gp1_D01A69520Dental Plaque (101)_NHPPlaqueD0Gp1Plaque_Gp1Plaque_D0_Gp1_Site12Box_1Box_1_D0
F20139.S004DP749_Gp1_D0DP749Dental Plaque (101)_NHPPlaqueD0Gp1Plaque_Gp1Plaque_D0_Gp1_Site12Box_1Box_1_D0
F20139.S005DP288_Gp1_D0DP288Dental Plaque (101)_NHPPlaqueD0Gp1Plaque_Gp1Plaque_D0_Gp1_Site12Box_1Box_1_D0
F20139.S006DV563_Gp2_D0DV563Dental Plaque (101)_NHPPlaqueD0Gp2Plaque_Gp2Plaque_D0_Gp2_Site12Box_1Box_1_D0
F20139.S007DV924_Gp2_D0DV924Dental Plaque (101)_NHPPlaqueD0Gp2Plaque_Gp2Plaque_D0_Gp2_Site12Box_1Box_1_D0
F20139.S008DP209_Gp2_D0DP209Dental Plaque (101)_NHPPlaqueD0Gp2Plaque_Gp2Plaque_D0_Gp2_Site12Box_1Box_1_D0
F20139.S0091B45521_Gp2_D01B45521Dental Plaque (101)_NHPPlaqueD0Gp2Plaque_Gp2Plaque_D0_Gp2_Site12Box_1Box_1_D0
F20139.S010DQ415_Gp2_D0DQ415Dental Plaque (101)_NHPPlaqueD0Gp2Plaque_Gp2Plaque_D0_Gp2_Site12Box_1Box_1_D0
F20139.S0111B51021_Gp1_D01B51021Dental Plaque (201)_NHPPlaqueD0Gp1Plaque_Gp1Plaque_D0_Gp1_Site12Box_1Box_1_D0
F20139.S012DV385_Gp1_D0DV385Dental Plaque (201)_NHPPlaqueD0Gp1Plaque_Gp1Plaque_D0_Gp1_Site12Box_1Box_1_D0
F20139.S0131A69520_Gp1_D01A69520Dental Plaque (201)_NHPPlaqueD0Gp1Plaque_Gp1Plaque_D0_Gp1_Site12Box_1Box_1_D0
F20139.S014DP749_Gp1_D0DP749Dental Plaque (201)_NHPPlaqueD0Gp1Plaque_Gp1Plaque_D0_Gp1_Site12Box_1Box_1_D0
F20139.S015DP288_Gp1_D0DP288Dental Plaque (201)_NHPPlaqueD0Gp1Plaque_Gp1Plaque_D0_Gp1_Site12Box_1Box_1_D0
F20139.S016DV563_Gp2_D0DV563Dental Plaque (201)_NHPPlaqueD0Gp2Plaque_Gp2Plaque_D0_Gp2_Site12Box_1Box_1_D0
F20139.S017DV924_Gp2_D0DV924Dental Plaque (201)_NHPPlaqueD0Gp2Plaque_Gp2Plaque_D0_Gp2_Site12Box_1Box_1_D0
F20139.S018DP209_Gp2_D0DP209Dental Plaque (201)_NHPPlaqueD0Gp2Plaque_Gp2Plaque_D0_Gp2_Site12Box_1Box_1_D0
F20139.S0191B45521_Gp2_D01B45521Dental Plaque (201)_NHPPlaqueD0Gp2Plaque_Gp2Plaque_D0_Gp2_Site12Box_1Box_1_D0
F20139.S020DQ415_Gp2_D0DQ415Dental Plaque (201)_NHPPlaqueD0Gp2Plaque_Gp2Plaque_D0_Gp2_Site12Box_1Box_1_D0
F20139.S0211B51021_Gp1_D01B51021Dental Plaque (301)_NHPPlaqueD0Gp1Plaque_Gp1Plaque_D0_Gp1_Site34Box_1Box_1_D0
F20139.S022DV385_Gp1_D0DV385Dental Plaque (301)_NHPPlaqueD0Gp1Plaque_Gp1Plaque_D0_Gp1_Site34Box_1Box_1_D0
F20139.S0231A69520_Gp1_D01A69520Dental Plaque (301)_NHPPlaqueD0Gp1Plaque_Gp1Plaque_D0_Gp1_Site34Box_1Box_1_D0
F20139.S024DP749_Gp1_D0DP749Dental Plaque (301)_NHPPlaqueD0Gp1Plaque_Gp1Plaque_D0_Gp1_Site34Box_1Box_1_D0
F20139.S025DP288_Gp1_D0DP288Dental Plaque (301)_NHPPlaqueD0Gp1Plaque_Gp1Plaque_D0_Gp1_Site34Box_1Box_1_D0
F20139.S026DV563_Gp2_D0DV563Dental Plaque (301)_NHPPlaqueD0Gp2Plaque_Gp2Plaque_D0_Gp2_Site34Box_1Box_1_D0
F20139.S027DV924_Gp2_D0DV924Dental Plaque (301)_NHPPlaqueD0Gp2Plaque_Gp2Plaque_D0_Gp2_Site34Box_1Box_1_D0
F20139.S028DP209_Gp2_D0DP209Dental Plaque (301)_NHPPlaqueD0Gp2Plaque_Gp2Plaque_D0_Gp2_Site34Box_1Box_1_D0
F20139.S0291B45521_Gp2_D01B45521Dental Plaque (301)_NHPPlaqueD0Gp2Plaque_Gp2Plaque_D0_Gp2_Site34Box_1Box_1_D0
F20139.S030DQ415_Gp2_D0DQ415Dental Plaque (301)_NHPPlaqueD0Gp2Plaque_Gp2Plaque_D0_Gp2_Site34Box_1Box_1_D0
F20139.S0311B51021_Gp1_D01B51021Dental Plaque (401)_NHPPlaqueD0Gp1Plaque_Gp1Plaque_D0_Gp1_Site34Box_1Box_1_D0
F20139.S032DV385_Gp1_D0DV385Dental Plaque (401)_NHPPlaqueD0Gp1Plaque_Gp1Plaque_D0_Gp1_Site34Box_1Box_1_D0
F20139.S0331A69520_Gp1_D01A69520Dental Plaque (401)_NHPPlaqueD0Gp1Plaque_Gp1Plaque_D0_Gp1_Site34Box_1Box_1_D0
F20139.S034DP749_Gp1_D0DP749Dental Plaque (401)_NHPPlaqueD0Gp1Plaque_Gp1Plaque_D0_Gp1_Site34Box_1Box_1_D0
F20139.S035DP288_Gp1_D0DP288Dental Plaque (401)_NHPPlaqueD0Gp1Plaque_Gp1Plaque_D0_Gp1_Site34Box_1Box_1_D0
F20139.S036DV563_Gp2_D0DV563Dental Plaque (401)_NHPPlaqueD0Gp2Plaque_Gp2Plaque_D0_Gp2_Site34Box_1Box_1_D0
F20139.S037DV924_Gp2_D0DV924Dental Plaque (401)_NHPPlaqueD0Gp2Plaque_Gp2Plaque_D0_Gp2_Site34Box_1Box_1_D0
F20139.S038DP209_Gp2_D0DP209Dental Plaque (401)_NHPPlaqueD0Gp2Plaque_Gp2Plaque_D0_Gp2_Site34Box_1Box_1_D0
F20139.S0391B45521_Gp2_D01B45521Dental Plaque (401)_NHPPlaqueD0Gp2Plaque_Gp2Plaque_D0_Gp2_Site34Box_1Box_1_D0
F20139.S040DQ415_Gp2_D0DQ415Dental Plaque (401)_NHPPlaqueD0Gp2Plaque_Gp2Plaque_D0_Gp2_Site34Box_1Box_1_D0
F20139.S0411B51021_Gp1_D561B51021Dental Plaque (101)_NHPPlaqueD56Gp1Plaque_Gp1Plaque_D56_Gp1_Site12Box_2Box_2_D56
F20139.S042DV385_Gp1_D56DV385Dental Plaque (101)_NHPPlaqueD56Gp1Plaque_Gp1Plaque_D56_Gp1_Site12Box_2Box_2_D56
F20139.S0431A69520_Gp1_D561A69520Dental Plaque (101)_NHPPlaqueD56Gp1Plaque_Gp1Plaque_D56_Gp1_Site12Box_2Box_2_D56
F20139.S044DP749_Gp1_D56DP749Dental Plaque (101)_NHPPlaqueD56Gp1Plaque_Gp1Plaque_D56_Gp1_Site12Box_2Box_2_D56
F20139.S045DP288_Gp1_D56DP288Dental Plaque (101)_NHPPlaqueD56Gp1Plaque_Gp1Plaque_D56_Gp1_Site12Box_2Box_2_D56
F20139.S046DV563_Gp2_D56DV563Dental Plaque (101)_NHPPlaqueD56Gp2Plaque_Gp2Plaque_D56_Gp2_Site12Box_2Box_2_D56
F20139.S047DV924_Gp2_D56DV924Dental Plaque (101)_NHPPlaqueD56Gp2Plaque_Gp2Plaque_D56_Gp2_Site12Box_2Box_2_D56
F20139.S048DP209_Gp2_D56DP209Dental Plaque (101)_NHPPlaqueD56Gp2Plaque_Gp2Plaque_D56_Gp2_Site12Box_2Box_2_D56
F20139.S0491B45521_Gp2_D561B45521Dental Plaque (101)_NHPPlaqueD56Gp2Plaque_Gp2Plaque_D56_Gp2_Site12Box_2Box_2_D56
F20139.S050DQ415_Gp2_D56DQ415Dental Plaque (101)_NHPPlaqueD56Gp2Plaque_Gp2Plaque_D56_Gp2_Site12Box_2Box_2_D56
F20139.S0511B51021_Gp1_D561B51021Dental Plaque (201)_NHPPlaqueD56Gp1Plaque_Gp1Plaque_D56_Gp1_Site12Box_2Box_2_D56
F20139.S052DV385_Gp1_D56DV385Dental Plaque (201)_NHPPlaqueD56Gp1Plaque_Gp1Plaque_D56_Gp1_Site12Box_2Box_2_D56
F20139.S0531A69520_Gp1_D561A69520Dental Plaque (201)_NHPPlaqueD56Gp1Plaque_Gp1Plaque_D56_Gp1_Site12Box_2Box_2_D56
F20139.S054DP749_Gp1_D56DP749Dental Plaque (201)_NHPPlaqueD56Gp1Plaque_Gp1Plaque_D56_Gp1_Site12Box_2Box_2_D56
F20139.S055DP288_Gp1_D56DP288Dental Plaque (201)_NHPPlaqueD56Gp1Plaque_Gp1Plaque_D56_Gp1_Site12Box_2Box_2_D56
F20139.S056DV563_Gp2_D56DV563Dental Plaque (201)_NHPPlaqueD56Gp2Plaque_Gp2Plaque_D56_Gp2_Site12Box_2Box_2_D56
F20139.S057DV924_Gp2_D56DV924Dental Plaque (201)_NHPPlaqueD56Gp2Plaque_Gp2Plaque_D56_Gp2_Site12Box_2Box_2_D56
F20139.S058DP209_Gp2_D56DP209Dental Plaque (201)_NHPPlaqueD56Gp2Plaque_Gp2Plaque_D56_Gp2_Site12Box_2Box_2_D56
F20139.S0591B45521_Gp2_D561B45521Dental Plaque (201)_NHPPlaqueD56Gp2Plaque_Gp2Plaque_D56_Gp2_Site12Box_2Box_2_D56
F20139.S060DQ415_Gp2_D56DQ415Dental Plaque (201)_NHPPlaqueD56Gp2Plaque_Gp2Plaque_D56_Gp2_Site12Box_2Box_2_D56
F20139.S0611B51021_Gp1_D561B51021Dental Plaque (301)_NHPPlaqueD56Gp1Plaque_Gp1Plaque_D56_Gp1_Site34Box_2Box_2_D56
F20139.S062DV385_Gp1_D56DV385Dental Plaque (301)_NHPPlaqueD56Gp1Plaque_Gp1Plaque_D56_Gp1_Site34Box_2Box_2_D56
F20139.S0631A69520_Gp1_D561A69520Dental Plaque (301)_NHPPlaqueD56Gp1Plaque_Gp1Plaque_D56_Gp1_Site34Box_2Box_2_D56
F20139.S064DP749_Gp1_D56DP749Dental Plaque (301)_NHPPlaqueD56Gp1Plaque_Gp1Plaque_D56_Gp1_Site34Box_2Box_2_D56
F20139.S065DP288_Gp1_D56DP288Dental Plaque (301)_NHPPlaqueD56Gp1Plaque_Gp1Plaque_D56_Gp1_Site34Box_2Box_2_D56
F20139.S066DV563_Gp2_D56DV563Dental Plaque (301)_NHPPlaqueD56Gp2Plaque_Gp2Plaque_D56_Gp2_Site34Box_2Box_2_D56
F20139.S067DV924_Gp2_D56DV924Dental Plaque (301)_NHPPlaqueD56Gp2Plaque_Gp2Plaque_D56_Gp2_Site34Box_2Box_2_D56
F20139.S068DP209_Gp2_D56DP209Dental Plaque (301)_NHPPlaqueD56Gp2Plaque_Gp2Plaque_D56_Gp2_Site34Box_2Box_2_D56
F20139.S0691B45521_Gp2_D561B45521Dental Plaque (301)_NHPPlaqueD56Gp2Plaque_Gp2Plaque_D56_Gp2_Site34Box_2Box_2_D56
F20139.S070DQ415_Gp2_D56DQ415Dental Plaque (301)_NHPPlaqueD56Gp2Plaque_Gp2Plaque_D56_Gp2_Site34Box_2Box_2_D56
F20139.S0711B51021_Gp1_D561B51021Dental Plaque (401)_NHPPlaqueD56Gp1Plaque_Gp1Plaque_D56_Gp1_Site34Box_2Box_2_D56
F20139.S072DV385_Gp1_D56DV385Dental Plaque (401)_NHPPlaqueD56Gp1Plaque_Gp1Plaque_D56_Gp1_Site34Box_2Box_2_D56
F20139.S0731A69520_Gp1_D561A69520Dental Plaque (401)_NHPPlaqueD56Gp1Plaque_Gp1Plaque_D56_Gp1_Site34Box_2Box_2_D56
F20139.S074DP749_Gp1_D56DP749Dental Plaque (401)_NHPPlaqueD56Gp1Plaque_Gp1Plaque_D56_Gp1_Site34Box_2Box_2_D56
F20139.S075DP288_Gp1_D56DP288Dental Plaque (401)_NHPPlaqueD56Gp1Plaque_Gp1Plaque_D56_Gp1_Site34Box_2Box_2_D56
F20139.S076DV563_Gp2_D56DV563Dental Plaque (401)_NHPPlaqueD56Gp2Plaque_Gp2Plaque_D56_Gp2_Site34Box_2Box_2_D56
F20139.S077DV924_Gp2_D56DV924Dental Plaque (401)_NHPPlaqueD56Gp2Plaque_Gp2Plaque_D56_Gp2_Site34Box_2Box_2_D56
F20139.S078DP209_Gp2_D56DP209Dental Plaque (401)_NHPPlaqueD56Gp2Plaque_Gp2Plaque_D56_Gp2_Site34Box_2Box_2_D56
F20139.S0791B45521_Gp2_D561B45521Dental Plaque (401)_NHPPlaqueD56Gp2Plaque_Gp2Plaque_D56_Gp2_Site34Box_2Box_2_D56
F20139.S080DQ415_Gp2_D56DQ415Dental Plaque (401)_NHPPlaqueD56Gp2Plaque_Gp2Plaque_D56_Gp2_Site34Box_2Box_2_D56
F20139.S0811B51021_Gp 1_D701B51021Dental Plaque (101)_NHPPlaqueD70Gp1Plaque_Gp1Plaque_D70_Gp1_Site12Box_3Box_3_D70
F20139.S082DV385__Gp 1_D70DV385Dental Plaque (101)_NHPPlaqueD70Gp1Plaque_Gp1Plaque_D70_Gp1_Site12Box_3Box_3_D70
F20139.S0831A69520_Gp 1_D701A69520Dental Plaque (101)_NHPPlaqueD70Gp1Plaque_Gp1Plaque_D70_Gp1_Site12Box_3Box_3_D70
F20139.S084DP749_Gp 1_D70DP749Dental Plaque (101)_NHPPlaqueD70Gp1Plaque_Gp1Plaque_D70_Gp1_Site12Box_3Box_3_D70
F20139.S085DP288_Gp 1_D70DP288Dental Plaque (101)_NHPPlaqueD70Gp1Plaque_Gp1Plaque_D70_Gp1_Site12Box_3Box_3_D70
F20139.S086DV563_Gp 2_D70DV563Dental Plaque (101)_NHPPlaqueD70Gp2Plaque_Gp2Plaque_D70_Gp2_Site12Box_3Box_3_D70
F20139.S087DV924_Gp 2_D70DV924Dental Plaque (101)_NHPPlaqueD70Gp2Plaque_Gp2Plaque_D70_Gp2_Site12Box_3Box_3_D70
F20139.S088DP209_Gp 2_D70DP209Dental Plaque (101)_NHPPlaqueD70Gp2Plaque_Gp2Plaque_D70_Gp2_Site12Box_3Box_3_D70
F20139.S0891B45521_Gp 2_D701B45521Dental Plaque (101)_NHPPlaqueD70Gp2Plaque_Gp2Plaque_D70_Gp2_Site12Box_3Box_3_D70
F20139.S090DQ415_Gp 2_D70DQ415Dental Plaque (101)_NHPPlaqueD70Gp2Plaque_Gp2Plaque_D70_Gp2_Site12Box_3Box_3_D70
F20139.S0911B51021_Gp 1_D701B51021Dental Plaque (201)_NHPPlaqueD70Gp1Plaque_Gp1Plaque_D70_Gp1_Site12Box_3Box_3_D70
F20139.S092DV385__Gp 1_D70DV385Dental Plaque (201)_NHPPlaqueD70Gp1Plaque_Gp1Plaque_D70_Gp1_Site12Box_3Box_3_D70
F20139.S0931A69520_Gp 1_D701A69520Dental Plaque (201)_NHPPlaqueD70Gp1Plaque_Gp1Plaque_D70_Gp1_Site12Box_3Box_3_D70
F20139.S094DP749_Gp 1_D70DP749Dental Plaque (201)_NHPPlaqueD70Gp1Plaque_Gp1Plaque_D70_Gp1_Site12Box_3Box_3_D70
F20139.S095DP288_Gp 1_D70DP288Dental Plaque (201)_NHPPlaqueD70Gp1Plaque_Gp1Plaque_D70_Gp1_Site12Box_3Box_3_D70
F20139.S096DV563_Gp 2_D70DV563Dental Plaque (201)_NHPPlaqueD70Gp2Plaque_Gp2Plaque_D70_Gp2_Site12Box_3Box_3_D70
F20139.S097DV924_Gp 2_D70DV924Dental Plaque (201)_NHPPlaqueD70Gp2Plaque_Gp2Plaque_D70_Gp2_Site12Box_3Box_3_D70
F20139.S098DP209_Gp 2_D70DP209Dental Plaque (201)_NHPPlaqueD70Gp2Plaque_Gp2Plaque_D70_Gp2_Site12Box_3Box_3_D70
F20139.S0991B45521_Gp 2_D701B45521Dental Plaque (201)_NHPPlaqueD70Gp2Plaque_Gp2Plaque_D70_Gp2_Site12Box_3Box_3_D70
F20139.S100DQ415_Gp 2_D70DQ415Dental Plaque (201)_NHPPlaqueD70Gp2Plaque_Gp2Plaque_D70_Gp2_Site12Box_3Box_3_D70
F20139.S1011B51021_Gp 1_D701B51021Dental Plaque (301)_NHPPlaqueD70Gp1Plaque_Gp1Plaque_D70_Gp1_Site34Box_3Box_3_D70
F20139.S102DV385__Gp 1_D70DV385Dental Plaque (301)_NHPPlaqueD70Gp1Plaque_Gp1Plaque_D70_Gp1_Site34Box_3Box_3_D70
F20139.S1031A69520_Gp 1_D701A69520Dental Plaque (301)_NHPPlaqueD70Gp1Plaque_Gp1Plaque_D70_Gp1_Site34Box_3Box_3_D70
F20139.S104DP749_Gp 1_D70DP749Dental Plaque (301)_NHPPlaqueD70Gp1Plaque_Gp1Plaque_D70_Gp1_Site34Box_3Box_3_D70
F20139.S105DP288_Gp 1_D70DP288Dental Plaque (301)_NHPPlaqueD70Gp1Plaque_Gp1Plaque_D70_Gp1_Site34Box_3Box_3_D70
F20139.S106DV563_Gp 2_D70DV563Dental Plaque (301)_NHPPlaqueD70Gp2Plaque_Gp2Plaque_D70_Gp2_Site34Box_3Box_3_D70
F20139.S107DV924_Gp 2_D70DV924Dental Plaque (301)_NHPPlaqueD70Gp2Plaque_Gp2Plaque_D70_Gp2_Site34Box_3Box_3_D70
F20139.S108DP209_Gp 2_D70DP209Dental Plaque (301)_NHPPlaqueD70Gp2Plaque_Gp2Plaque_D70_Gp2_Site34Box_3Box_3_D70
F20139.S1091B45521_Gp 2_D701B45521Dental Plaque (301)_NHPPlaqueD70Gp2Plaque_Gp2Plaque_D70_Gp2_Site34Box_3Box_3_D70
F20139.S110DQ415_Gp 2_D70DQ415Dental Plaque (301)_NHPPlaqueD70Gp2Plaque_Gp2Plaque_D70_Gp2_Site34Box_3Box_3_D70
F20139.S1111B51021_Gp 1_D701B51021Dental Plaque (401)_NHPPlaqueD70Gp1Plaque_Gp1Plaque_D70_Gp1_Site34Box_3Box_3_D70
F20139.S112DV385__Gp 1_D70DV385Dental Plaque (401)_NHPPlaqueD70Gp1Plaque_Gp1Plaque_D70_Gp1_Site34Box_3Box_3_D70
F20139.S1131A69520_Gp 1_D701A69520Dental Plaque (401)_NHPPlaqueD70Gp1Plaque_Gp1Plaque_D70_Gp1_Site34Box_3Box_3_D70
F20139.S114DP749_Gp 1_D70DP749Dental Plaque (401)_NHPPlaqueD70Gp1Plaque_Gp1Plaque_D70_Gp1_Site34Box_3Box_3_D70
F20139.S115DP288_Gp 1_D70DP288Dental Plaque (401)_NHPPlaqueD70Gp1Plaque_Gp1Plaque_D70_Gp1_Site34Box_3Box_3_D70
F20139.S116DV563_Gp 2_D70DV563Dental Plaque (401)_NHPPlaqueD70Gp2Plaque_Gp2Plaque_D70_Gp2_Site34Box_3Box_3_D70
F20139.S117DV924_Gp 2_D70DV924Dental Plaque (401)_NHPPlaqueD70Gp2Plaque_Gp2Plaque_D70_Gp2_Site34Box_3Box_3_D70
F20139.S118DP209_Gp 2_D70DP209Dental Plaque (401)_NHPPlaqueD70Gp2Plaque_Gp2Plaque_D70_Gp2_Site34Box_3Box_3_D70
F20139.S1191B45521_Gp 2_D701B45521Dental Plaque (401)_NHPPlaqueD70Gp2Plaque_Gp2Plaque_D70_Gp2_Site34Box_3Box_3_D70
F20139.S120DQ415_Gp 2_D70DQ415Dental Plaque (401)_NHPPlaqueD70Gp2Plaque_Gp2Plaque_D70_Gp2_Site34Box_3Box_3_D70
F20139.S1211B51021_Gp 1_D911B51021Dental Plaque (101)_NHPPlaqueD91Gp1Plaque_Gp1Plaque_D91_Gp1_Site12Box_4Box_4_D91
F20139.S122DV385_Gp 1_D91DV385Dental Plaque (101)_NHPPlaqueD91Gp1Plaque_Gp1Plaque_D91_Gp1_Site12Box_4Box_4_D91
F20139.S1231A69520_Gp 1_D911A69520Dental Plaque (101)_NHPPlaqueD91Gp1Plaque_Gp1Plaque_D91_Gp1_Site12Box_4Box_4_D91
F20139.S124DP749_Gp 1_D91DP749Dental Plaque (101)_NHPPlaqueD91Gp1Plaque_Gp1Plaque_D91_Gp1_Site12Box_4Box_4_D91
F20139.S125DP288_Gp 1_D91DP288Dental Plaque (101)_NHPPlaqueD91Gp1Plaque_Gp1Plaque_D91_Gp1_Site12Box_4Box_4_D91
F20139.S126DV563_Gp 2_D91DV563Dental Plaque (101)_NHPPlaqueD91Gp2Plaque_Gp2Plaque_D91_Gp2_Site12Box_4Box_4_D91
F20139.S127DV924_Gp 2_D91DV924Dental Plaque (101)_NHPPlaqueD91Gp2Plaque_Gp2Plaque_D91_Gp2_Site12Box_4Box_4_D91
F20139.S128DP209_Gp 2_D91DP209Dental Plaque (101)_NHPPlaqueD91Gp2Plaque_Gp2Plaque_D91_Gp2_Site12Box_4Box_4_D91
F20139.S1291B45521_Gp 2_D911B45521Dental Plaque (101)_NHPPlaqueD91Gp2Plaque_Gp2Plaque_D91_Gp2_Site12Box_4Box_4_D91
F20139.S130DQ415_Gp 2_D91DQ415Dental Plaque (101)_NHPPlaqueD91Gp2Plaque_Gp2Plaque_D91_Gp2_Site12Box_4Box_4_D91
F20139.S1311B51021_Gp 1_D911B51021Dental Plaque (201)_NHPPlaqueD91Gp1Plaque_Gp1Plaque_D91_Gp1_Site12Box_4Box_4_D91
F20139.S132DV385_Gp 1_D91DV385Dental Plaque (201)_NHPPlaqueD91Gp1Plaque_Gp1Plaque_D91_Gp1_Site12Box_4Box_4_D91
F20139.S1331A69520_Gp 1_D911A69520Dental Plaque (201)_NHPPlaqueD91Gp1Plaque_Gp1Plaque_D91_Gp1_Site12Box_4Box_4_D91
F20139.S134DP749_Gp 1_D91DP749Dental Plaque (201)_NHPPlaqueD91Gp1Plaque_Gp1Plaque_D91_Gp1_Site12Box_4Box_4_D91
F20139.S135DP288_Gp 1_D91DP288Dental Plaque (201)_NHPPlaqueD91Gp1Plaque_Gp1Plaque_D91_Gp1_Site12Box_4Box_4_D91
F20139.S136DV563_Gp 2_D91DV563Dental Plaque (201)_NHPPlaqueD91Gp2Plaque_Gp2Plaque_D91_Gp2_Site12Box_4Box_4_D91
F20139.S137DV924_Gp 2_D91DV924Dental Plaque (201)_NHPPlaqueD91Gp2Plaque_Gp2Plaque_D91_Gp2_Site12Box_4Box_4_D91
F20139.S138DP209_Gp 2_D91DP209Dental Plaque (201)_NHPPlaqueD91Gp2Plaque_Gp2Plaque_D91_Gp2_Site12Box_4Box_4_D91
F20139.S1391B45521_Gp 2_D911B45521Dental Plaque (201)_NHPPlaqueD91Gp2Plaque_Gp2Plaque_D91_Gp2_Site12Box_4Box_4_D91
F20139.S140DQ415_Gp 2_D91DQ415Dental Plaque (201)_NHPPlaqueD91Gp2Plaque_Gp2Plaque_D91_Gp2_Site12Box_4Box_4_D91
F20139.S1411B51021_Gp 1_D911B51021Dental Plaque (301)_NHPPlaqueD91Gp1Plaque_Gp1Plaque_D91_Gp1_Site34Box_4Box_4_D91
F20139.S142DV385_Gp 1_D91DV385Dental Plaque (301)_NHPPlaqueD91Gp1Plaque_Gp1Plaque_D91_Gp1_Site34Box_4Box_4_D91
F20139.S1431A69520_Gp 1_D911A69520Dental Plaque (301)_NHPPlaqueD91Gp1Plaque_Gp1Plaque_D91_Gp1_Site34Box_4Box_4_D91
F20139.S144DP749_Gp 1_D91DP749Dental Plaque (301)_NHPPlaqueD91Gp1Plaque_Gp1Plaque_D91_Gp1_Site34Box_4Box_4_D91
F20139.S145DP288_Gp 1_D91DP288Dental Plaque (301)_NHPPlaqueD91Gp1Plaque_Gp1Plaque_D91_Gp1_Site34Box_4Box_4_D91
F20139.S146DV563_Gp 2_D91DV563Dental Plaque (301)_NHPPlaqueD91Gp2Plaque_Gp2Plaque_D91_Gp2_Site34Box_4Box_4_D91
F20139.S147DV924_Gp 2_D91DV924Dental Plaque (301)_NHPPlaqueD91Gp2Plaque_Gp2Plaque_D91_Gp2_Site34Box_4Box_4_D91
F20139.S148DP209_Gp 2_D91DP209Dental Plaque (301)_NHPPlaqueD91Gp2Plaque_Gp2Plaque_D91_Gp2_Site34Box_4Box_4_D91
F20139.S1491B45521_Gp 2_D911B45521Dental Plaque (301)_NHPPlaqueD91Gp2Plaque_Gp2Plaque_D91_Gp2_Site34Box_4Box_4_D91
F20139.S150DQ415_Gp 2_D91DQ415Dental Plaque (301)_NHPPlaqueD91Gp2Plaque_Gp2Plaque_D91_Gp2_Site34Box_4Box_4_D91
F20139.S1511B51021_Gp 1_D911B51021Dental Plaque (401)_NHPPlaqueD91Gp1Plaque_Gp1Plaque_D91_Gp1_Site34Box_4Box_4_D91
F20139.S152DV385_Gp 1_D91DV385Dental Plaque (401)_NHPPlaqueD91Gp1Plaque_Gp1Plaque_D91_Gp1_Site34Box_4Box_4_D91
F20139.S1531A69520_Gp 1_D911A69520Dental Plaque (401)_NHPPlaqueD91Gp1Plaque_Gp1Plaque_D91_Gp1_Site34Box_4Box_4_D91
F20139.S154DP749_Gp 1_D91DP749Dental Plaque (401)_NHPPlaqueD91Gp1Plaque_Gp1Plaque_D91_Gp1_Site34Box_4Box_4_D91
F20139.S155DP288_Gp 1_D91DP288Dental Plaque (401)_NHPPlaqueD91Gp1Plaque_Gp1Plaque_D91_Gp1_Site34Box_4Box_4_D91
F20139.S156DV563_Gp 2_D91DV563Dental Plaque (401)_NHPPlaqueD91Gp2Plaque_Gp2Plaque_D91_Gp2_Site34Box_4Box_4_D91
F20139.S157DV924_Gp 2_D91DV924Dental Plaque (401)_NHPPlaqueD91Gp2Plaque_Gp2Plaque_D91_Gp2_Site34Box_4Box_4_D91
F20139.S158DP209_Gp 2_D91DP209Dental Plaque (401)_NHPPlaqueD91Gp2Plaque_Gp2Plaque_D91_Gp2_Site34Box_4Box_4_D91
F20139.S1591B45521_Gp 2_D911B45521Dental Plaque (401)_NHPPlaqueD91Gp2Plaque_Gp2Plaque_D91_Gp2_Site34Box_4Box_4_D91
F20139.S160DQ415_Gp 2_D91DQ415Dental Plaque (401)_NHPPlaqueD91Gp2Plaque_Gp2Plaque_D91_Gp2_Site34Box_4Box_4_D91
F20139.S1611B51021_Gp1_D01B51021Saliva-NHPSalivaD0Gp1Saliva_Gp1Saliva_D0_Gp1Box_5Box_5_D0
F20139.S162DV385_Gp1_D0DV385Saliva-NHPSalivaD0Gp1Saliva_Gp1Saliva_D0_Gp1Box_5Box_5_D0
F20139.S1631A69520_Gp1_D01A69520Saliva-NHPSalivaD0Gp1Saliva_Gp1Saliva_D0_Gp1Box_5Box_5_D0
F20139.S164DP749_Gp1_D0DP749Saliva-NHPSalivaD0Gp1Saliva_Gp1Saliva_D0_Gp1Box_5Box_5_D0
F20139.S165DP288_Gp1_D0DP288Saliva-NHPSalivaD0Gp1Saliva_Gp1Saliva_D0_Gp1Box_5Box_5_D0
F20139.S166DV563_Gp2_D0DV563Saliva-NHPSalivaD0Gp2Saliva_Gp2Saliva_D0_Gp2Box_5Box_5_D0
F20139.S167DV924_Gp2_D0DV924Saliva-NHPSalivaD0Gp2Saliva_Gp2Saliva_D0_Gp2Box_5Box_5_D0
F20139.S168DP209_Gp2_D0DP209Saliva-NHPSalivaD0Gp2Saliva_Gp2Saliva_D0_Gp2Box_5Box_5_D0
F20139.S1691B45521_Gp2_D01B45521Saliva-NHPSalivaD0Gp2Saliva_Gp2Saliva_D0_Gp2Box_5Box_5_D0
F20139.S170DQ415_Gp2_D0DQ415Saliva-NHPSalivaD0Gp2Saliva_Gp2Saliva_D0_Gp2Box_5Box_5_D0
F20139.S1711B51021_Gp1_D561B51021Saliva-NHPSalivaD56Gp1Saliva_Gp1Saliva_D56_Gp1Box_5Box_5_D56
F20139.S172DV385_Gp1_D56DV385Saliva-NHPSalivaD56Gp1Saliva_Gp1Saliva_D56_Gp1Box_5Box_5_D56
F20139.S1731A69520_Gp1_D561A69520Saliva-NHPSalivaD56Gp1Saliva_Gp1Saliva_D56_Gp1Box_5Box_5_D56
F20139.S174DP749_Gp1_D56DP749Saliva-NHPSalivaD56Gp1Saliva_Gp1Saliva_D56_Gp1Box_5Box_5_D56
F20139.S175DP288_Gp1_D56DP288Saliva-NHPSalivaD56Gp1Saliva_Gp1Saliva_D56_Gp1Box_5Box_5_D56
F20139.S176DV563_Gp2_D56DV563Saliva-NHPSalivaD56Gp2Saliva_Gp2Saliva_D56_Gp2Box_5Box_5_D56
F20139.S177DV924_Gp2_D56DV924Saliva-NHPSalivaD56Gp2Saliva_Gp2Saliva_D56_Gp2Box_5Box_5_D56
F20139.S178DP209_Gp2_D56DP209Saliva-NHPSalivaD56Gp2Saliva_Gp2Saliva_D56_Gp2Box_5Box_5_D56
F20139.S1791B45521_Gp2_D561B45521Saliva-NHPSalivaD56Gp2Saliva_Gp2Saliva_D56_Gp2Box_5Box_5_D56
F20139.S180DQ415_Gp2_D56DQ415Saliva-NHPSalivaD56Gp2Saliva_Gp2Saliva_D56_Gp2Box_5Box_5_D56
F20139.S1811B51021_Gp 1_D701B51021Saliva-NHPSalivaD70Gp1Saliva_Gp1Saliva_D70_Gp1Box_5Box_5_D70
F20139.S182DV385__Gp 1_D70DV385Saliva-NHPSalivaD70Gp1Saliva_Gp1Saliva_D70_Gp1Box_5Box_5_D70
F20139.S1831A69520_Gp 1_D701A69520Saliva-NHPSalivaD70Gp1Saliva_Gp1Saliva_D70_Gp1Box_5Box_5_D70
F20139.S184DP749_Gp 1_D70DP749Saliva-NHPSalivaD70Gp1Saliva_Gp1Saliva_D70_Gp1Box_5Box_5_D70
F20139.S185DP288_Gp 1_D70DP288Saliva-NHPSalivaD70Gp1Saliva_Gp1Saliva_D70_Gp1Box_5Box_5_D70
F20139.S186DV563_Gp 2_D70DV563Saliva-NHPSalivaD70Gp2Saliva_Gp2Saliva_D70_Gp2Box_5Box_5_D70
F20139.S187DV924_Gp 2_D70DV924Saliva-NHPSalivaD70Gp2Saliva_Gp2Saliva_D70_Gp2Box_5Box_5_D70
F20139.S188DP209_Gp 2_D70DP209Saliva-NHPSalivaD70Gp2Saliva_Gp2Saliva_D70_Gp2Box_5Box_5_D70
F20139.S1891B45521_Gp 2_D701B45521Saliva-NHPSalivaD70Gp2Saliva_Gp2Saliva_D70_Gp2Box_5Box_5_D70
F20139.S190DQ415_Gp 2_D70DQ415Saliva-NHPSalivaD70Gp2Saliva_Gp2Saliva_D70_Gp2Box_5Box_5_D70
F20139.S1911B51021_Gp 1_D911B51021Saliva-NHPSalivaD91Gp1Saliva_Gp1Saliva_D91_Gp1Box_5Box_5_D91
F20139.S192DV385_Gp 1_D91DV385Saliva-NHPSalivaD91Gp1Saliva_Gp1Saliva_D91_Gp1Box_5Box_5_D91
F20139.S1931A69520_Gp 1_D911A69520Saliva-NHPSalivaD91Gp1Saliva_Gp1Saliva_D91_Gp1Box_5Box_5_D91
F20139.S194DP749_Gp 1_D91DP749Saliva-NHPSalivaD91Gp1Saliva_Gp1Saliva_D91_Gp1Box_5Box_5_D91
F20139.S195DP288_Gp 1_D91DP288Saliva-NHPSalivaD91Gp1Saliva_Gp1Saliva_D91_Gp1Box_5Box_5_D91
F20139.S196DV563_Gp 2_D91DV563Saliva-NHPSalivaD91Gp2Saliva_Gp2Saliva_D91_Gp2Box_5Box_5_D91
F20139.S197DV924_Gp 2_D91DV924Saliva-NHPSalivaD91Gp2Saliva_Gp2Saliva_D91_Gp2Box_5Box_5_D91
F20139.S198DP209_Gp 2_D91DP209Saliva-NHPSalivaD91Gp2Saliva_Gp2Saliva_D91_Gp2Box_5Box_5_D91
F20139.S1991B45521_Gp 2_D911B45521Saliva-NHPSalivaD91Gp2Saliva_Gp2Saliva_D91_Gp2Box_5Box_5_D91
F20139.S200DQ415_Gp 2_D91DQ415Saliva-NHPSalivaD91Gp2Saliva_Gp2Saliva_D91_Gp2Box_5Box_5_D91
F20139.S2011B51021_Gp1_D01B51021Feces_NHPFecesD0Gp1Feces_Gp1Feces_D0_Gp1Box_6Box_6_D0
F20139.S202DV385_Gp1_D0DV385Feces_NHPFecesD0Gp1Feces_Gp1Feces_D0_Gp1Box_6Box_6_D0
F20139.S2031A69520_Gp1_D01A69520Feces_NHPFecesD0Gp1Feces_Gp1Feces_D0_Gp1Box_6Box_6_D0
F20139.S204DP749_Gp1_D0DP749Feces_NHPFecesD0Gp1Feces_Gp1Feces_D0_Gp1Box_6Box_6_D0
F20139.S205DP288_Gp1_D0DP288Feces_NHPFecesD0Gp1Feces_Gp1Feces_D0_Gp1Box_6Box_6_D0
F20139.S206DV563_Gp2_D0DV563Feces_NHPFecesD0Gp2Feces_Gp2Feces_D0_Gp2Box_6Box_6_D0
F20139.S207DV924_Gp2_D0DV924Feces_NHPFecesD0Gp2Feces_Gp2Feces_D0_Gp2Box_6Box_6_D0
F20139.S208DP209_Gp2_D0DP209Feces_NHPFecesD0Gp2Feces_Gp2Feces_D0_Gp2Box_6Box_6_D0
F20139.S2091B45521_Gp2_D01B45521Feces_NHPFecesD0Gp2Feces_Gp2Feces_D0_Gp2Box_6Box_6_D0
F20139.S210DQ415_Gp2_D0DQ415Feces_NHPFecesD0Gp2Feces_Gp2Feces_D0_Gp2Box_6Box_6_D0
F20139.S2111B51021_Gp1_D561B51021Feces_NHPFecesD56Gp1Feces_Gp1Feces_D56_Gp1Box_6Box_6_D56
F20139.S212DV385_Gp1_D56DV385Feces_NHPFecesD56Gp1Feces_Gp1Feces_D56_Gp1Box_6Box_6_D56
F20139.S2131A69520_Gp1_D561A69520Feces_NHPFecesD56Gp1Feces_Gp1Feces_D56_Gp1Box_6Box_6_D56
F20139.S214DP749_Gp1_D56DP749Feces_NHPFecesD56Gp1Feces_Gp1Feces_D56_Gp1Box_6Box_6_D56
F20139.S215DP288_Gp1_D56DP288Feces_NHPFecesD56Gp1Feces_Gp1Feces_D56_Gp1Box_6Box_6_D56
F20139.S216DV563_Gp2_D56DV563Feces_NHPFecesD56Gp2Feces_Gp2Feces_D56_Gp2Box_6Box_6_D56
F20139.S217DV924_Gp2_D56DV924Feces_NHPFecesD56Gp2Feces_Gp2Feces_D56_Gp2Box_6Box_6_D56
F20139.S218DP209_Gp2_D56DP209Feces_NHPFecesD56Gp2Feces_Gp2Feces_D56_Gp2Box_6Box_6_D56
F20139.S2191B45521_Gp2_D561B45521Feces_NHPFecesD56Gp2Feces_Gp2Feces_D56_Gp2Box_6Box_6_D56
F20139.S220DQ415_Gp2_D56DQ415Feces_NHPFecesD56Gp2Feces_Gp2Feces_D56_Gp2Box_6Box_6_D56
F20139.S2211B51021_Gp 1_D701B51021Feces_NHPFecesD70Gp1Feces_Gp1Feces_D70_Gp1Box_6Box_6_D70
F20139.S222DV385__Gp 1_D70DV385Feces_NHPFecesD70Gp1Feces_Gp1Feces_D70_Gp1Box_6Box_6_D70
F20139.S2231A69520_Gp 1_D701A69520Feces_NHPFecesD70Gp1Feces_Gp1Feces_D70_Gp1Box_6Box_6_D70
F20139.S224DP749_Gp 1_D70DP749Feces_NHPFecesD70Gp1Feces_Gp1Feces_D70_Gp1Box_6Box_6_D70
F20139.S225DP288_Gp 1_D70DP288Feces_NHPFecesD70Gp1Feces_Gp1Feces_D70_Gp1Box_6Box_6_D70
F20139.S226DV563_Gp 2_D70DV563Feces_NHPFecesD70Gp2Feces_Gp2Feces_D70_Gp2Box_6Box_6_D70
F20139.S227DV924_Gp 2_D70DV924Feces_NHPFecesD70Gp2Feces_Gp2Feces_D70_Gp2Box_6Box_6_D70
F20139.S228DP209_Gp 2_D70DP209Feces_NHPFecesD70Gp2Feces_Gp2Feces_D70_Gp2Box_6Box_6_D70
F20139.S2291B45521_Gp 2_D701B45521Feces_NHPFecesD70Gp2Feces_Gp2Feces_D70_Gp2Box_6Box_6_D70
F20139.S230DQ415_Gp 2_D70DQ415Feces_NHPFecesD70Gp2Feces_Gp2Feces_D70_Gp2Box_6Box_6_D70
F20139.S2311B51021_Gp 1_D911B51021Feces_NHPFecesD91Gp1Feces_Gp1Feces_D91_Gp1Box_6Box_6_D91
F20139.S232DV385_Gp 1_D91DV385Feces_NHPFecesD91Gp1Feces_Gp1Feces_D91_Gp1Box_6Box_6_D91
F20139.S2331A69520_Gp 1_D911A69520Feces_NHPFecesD91Gp1Feces_Gp1Feces_D91_Gp1Box_6Box_6_D91
F20139.S234DP749_Gp 1_D91DP749Feces_NHPFecesD91Gp1Feces_Gp1Feces_D91_Gp1Box_6Box_6_D91
F20139.S235DP288_Gp 1_D91DP288Feces_NHPFecesD91Gp1Feces_Gp1Feces_D91_Gp1Box_6Box_6_D91
F20139.S236DV563_Gp 2_D91DV563Feces_NHPFecesD91Gp2Feces_Gp2Feces_D91_Gp2Box_6Box_6_D91
F20139.S237DV924_Gp 2_D91DV924Feces_NHPFecesD91Gp2Feces_Gp2Feces_D91_Gp2Box_6Box_6_D91
F20139.S238DP209_Gp 2_D91DP209Feces_NHPFecesD91Gp2Feces_Gp2Feces_D91_Gp2Box_6Box_6_D91
F20139.S2391B45521_Gp 2_D911B45521Feces_NHPFecesD91Gp2Feces_Gp2Feces_D91_Gp2Box_6Box_6_D91
F20139.S240DQ415_Gp 2_D91DQ415Feces_NHPFecesD91Gp2Feces_Gp2Feces_D91_Gp2Box_6Box_6_D91
 
 

ASV Read Counts by Samples

#Sample IDRead Count
F20139.S20141,395
F20139.S20450,765
F20139.S22058,776
F20139.S21359,750
F20139.S20660,407
F20139.S22260,448
F20139.S21461,837
F20139.S20262,431
F20139.S21662,995
F20139.S21064,592
F20139.S20766,383
F20139.S20367,251
F20139.S20968,249
F20139.S22569,963
F20139.S23770,152
F20139.S22870,615
F20139.S07170,995
F20139.S23671,641
F20139.S22172,315
F20139.S21272,794
F20139.S23573,018
F20139.S23874,338
F20139.S20574,826
F20139.S21177,037
F20139.S07877,648
F20139.S22478,325
F20139.S21978,563
F20139.S16178,948
F20139.S04079,845
F20139.S23080,077
F20139.S18480,151
F20139.S16581,576
F20139.S22781,875
F20139.S16881,996
F20139.S21782,691
F20139.S00183,044
F20139.S18783,321
F20139.S19285,618
F20139.S09885,651
F20139.S19885,740
F20139.S00985,823
F20139.S12585,921
F20139.S09386,330
F20139.S24086,409
F20139.S18986,452
F20139.S11386,470
F20139.S23286,858
F20139.S14586,946
F20139.S15587,042
F20139.S01587,052
F20139.S21587,236
F20139.S13187,311
F20139.S17187,357
F20139.S16287,802
F20139.S11587,941
F20139.S00588,074
F20139.S09788,516
F20139.S18089,142
F20139.S10789,640
F20139.S13889,932
F20139.S09990,282
F20139.S14090,666
F20139.S23390,860
F20139.S09590,997
F20139.S03391,046
F20139.S22691,084
F20139.S03291,166
F20139.S02591,220
F20139.S22391,689
F20139.S08091,905
F20139.S17491,927
F20139.S11792,607
F20139.S12892,637
F20139.S14492,704
F20139.S02793,129
F20139.S18393,398
F20139.S12193,602
F20139.S10293,924
F20139.S20094,035
F20139.S08794,102
F20139.S15394,560
F20139.S14394,659
F20139.S17994,765
F20139.S16794,768
F20139.S23494,866
F20139.S17694,917
F20139.S10494,975
F20139.S06095,142
F20139.S17795,281
F20139.S08495,414
F20139.S13695,531
F20139.S01995,581
F20139.S08395,884
F20139.S11295,916
F20139.S14795,987
F20139.S00796,043
F20139.S18896,468
F20139.S23996,507
F20139.S15296,779
F20139.S12397,215
F20139.S06497,840
F20139.S13097,937
F20139.S07998,032
F20139.S14898,696
F20139.S15898,866
F20139.S04799,404
F20139.S20899,412
F20139.S03099,454
F20139.S01899,578
F20139.S12499,589
F20139.S03999,596
F20139.S134100,151
F20139.S086100,195
F20139.S129100,206
F20139.S122100,380
F20139.S014100,437
F20139.S031100,467
F20139.S110100,812
F20139.S010100,839
F20139.S126101,076
F20139.S156101,174
F20139.S139101,271
F20139.S091101,406
F20139.S199101,537
F20139.S229101,870
F20139.S076101,991
F20139.S072102,183
F20139.S048102,209
F20139.S074102,214
F20139.S166102,269
F20139.S151102,325
F20139.S114102,559
F20139.S197102,793
F20139.S175103,147
F20139.S017103,191
F20139.S163103,391
F20139.S006103,473
F20139.S191103,666
F20139.S037103,971
F20139.S170103,978
F20139.S038104,019
F20139.S035104,229
F20139.S013104,288
F20139.S011104,761
F20139.S127104,807
F20139.S120104,820
F20139.S186105,279
F20139.S023105,310
F20139.S094105,331
F20139.S034105,408
F20139.S150105,487
F20139.S105105,530
F20139.S012105,712
F20139.S101105,734
F20139.S052105,773
F20139.S218105,856
F20139.S055105,909
F20139.S109106,066
F20139.S231106,172
F20139.S075106,322
F20139.S088106,385
F20139.S066106,387
F20139.S063106,448
F20139.S036107,299
F20139.S169107,551
F20139.S008107,846
F20139.S058107,857
F20139.S090107,940
F20139.S077108,000
F20139.S004108,073
F20139.S132108,181
F20139.S100108,306
F20139.S196108,384
F20139.S043108,522
F20139.S056108,801
F20139.S149108,958
F20139.S044109,120
F20139.S106109,393
F20139.S096109,425
F20139.S142109,543
F20139.S173110,031
F20139.S159110,142
F20139.S160110,269
F20139.S024110,477
F20139.S108110,539
F20139.S111110,552
F20139.S164110,565
F20139.S141110,787
F20139.S135110,914
F20139.S178111,958
F20139.S051111,977
F20139.S182112,484
F20139.S119112,884
F20139.S103113,068
F20139.S146113,179
F20139.S020113,434
F20139.S154113,475
F20139.S073114,110
F20139.S057114,135
F20139.S116115,316
F20139.S172115,511
F20139.S089115,855
F20139.S041116,828
F20139.S003117,109
F20139.S081117,214
F20139.S118118,097
F20139.S042118,207
F20139.S193118,370
F20139.S029118,462
F20139.S137118,646
F20139.S133118,966
F20139.S059119,064
F20139.S185119,261
F20139.S065119,438
F20139.S050120,126
F20139.S053121,453
F20139.S016122,667
F20139.S085122,720
F20139.S190122,780
F20139.S061123,034
F20139.S049123,412
F20139.S022123,491
F20139.S021124,559
F20139.S045125,041
F20139.S157126,500
F20139.S068129,099
F20139.S046130,546
F20139.S054131,165
F20139.S070131,945
F20139.S026135,489
F20139.S092137,711
F20139.S062137,787
F20139.S195141,664
F20139.S028142,174
F20139.S002145,421
F20139.S181148,697
F20139.S194149,546
F20139.S067154,517
F20139.S082168,180
F20139.S069177,612
 
 
 

VII. Analysis - Read Taxonomy Assignment

Read Taxonomy Assignment - Methods

 

The close-reference taxonomy assignment of the ASV sequences using BLASTN is based on the algorithm published by Al-Hebshi et. al. (2015)[2].

The species-level, open-reference 16S rRNA NGS reads taxonomy assignment pipeline

Version 20210310a
 
 

1. Raw sequences reads in FASTA format were BLASTN-searched against a combined set of 16S rRNA reference sequences - the FOMC 16S rRNA Reference Sequences version 20221029 (https://microbiome.forsyth.org/ftp/refseq/). This set consists of the HOMD (version 15.22 http://www.homd.org/index.php?name=seqDownload&file&type=R ), Mouse Oral Microbiome Database (MOMD version 5.1 https://momd.org/ftp/16S_rRNA_refseq/MOMD_16S_rRNA_RefSeq/V5.1/), and the NCBI 16S rRNA reference sequence set (https://ftp.ncbi.nlm.nih.gov/blast/db/16S_ribosomal_RNA.tar.gz). These sequences were screened and combined to remove short sequences (<1000nt), chimera, duplicated and sub-sequences, as well as sequences with poor taxonomy annotation (e.g., without species information). This process resulted in 1,015 full-length 16S rRNA sequences from HOMD V15.22, 356 from MOMD V5.1, and 22,126 from NCBI, a total of 23,497 sequences. Altogether these sequence represent a total of 17,035 oral and non-oral microbial species.

The NCBI BLASTN version 2.7.1+ (Zhang et al, 2000) [3] was used with the default parameters. Reads with ≥ 98% sequence identity to the matched reference and ≥ 90% alignment length (i.e., ≥ 90% of the read length that was aligned to the reference and was used to calculate the sequence percent identity) were classified based on the taxonomy of the reference sequence with highest sequence identity. If a read matched with reference sequences representing more than one species with equal percent identity and alignment length, it was subject to chimera checking with USEARCH program version v8.1.1861 (Edgar 2010). Non-chimeric reads with multi-species best hits were considered valid and were assigned with a unique species notation (e.g., spp) denoting unresolvable multiple species.

2. Unassigned reads (i.e., reads with < 98% identity or < 90% alignment length) were pooled together and reads < 200 bases were removed. The remaining reads were subject to the de novo operational taxonomy unit (OTU) calling and chimera checking using the USEARCH program version v8.1.1861 (Edgar 2010)[4]. The de novo OTU calling and chimera checking was done using 98% as the sequence identity cutoff, i.e., the species-level OTU. The output of this step produced species-level de novo clustered OTUs with 98% identity. Representative reads from each of the OTUs/species were then BLASTN-searched against the same reference sequence set again to determine the closest species for these potential novel species. These potential novel species were pooled together with the reads that were signed to specie-level in the previous step, for down-stream analyses.

Reference:

  1. Al-Hebshi NN, Nasher AT, Idris AM, Chen T. Robust species taxonomy assignment algorithm for 16S rRNA NGS reads: application to oral carcinoma samples. J Oral Microbiol. 2015 Sep 29;7:28934. doi: 10.3402/jom.v7.28934. PMID: 26426306; PMCID: PMC4590409.
  2. Zhang Z, Schwartz S, Wagner L, Miller W. A greedy algorithm for aligning DNA sequences. J Comput Biol. 2000 Feb-Apr;7(1-2):203-14. doi: 10.1089/10665270050081478. PMID: 10890397.
  3. Edgar RC. Search and clustering orders of magnitude faster than BLAST. Bioinformatics. 2010 Oct 1;26(19):2460-1. doi: 10.1093/bioinformatics/btq461. Epub 2010 Aug 12. PubMed PMID: 20709691.

    4. 3. Designations used in the taxonomy:

    	1) Taxonomy levels are indicated by these prefixes:
	
	   k__: domain/kingdom
	   p__: phylum
	   c__: class
	   o__: order
	   f__: family
	   g__: genus  
	   s__: species
	
	   Example: 
	
	   k__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__Blautia;s__faecis
		
	2) Unique level identified – known species:
	   
	   k__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__Roseburia;s__hominis
	
	   The above example shows some reads match to a single species (all levels are unique)
	
	3) Non-unique level identified – known species:

	   k__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__Roseburia;s__multispecies_spp123_3
	   
	   The above example “s__multispecies_spp123_3” indicates certain reads equally match to 3 species of the 
	   genus Roseburia; the “spp123” is a temporally assigned species ID.
	
	   k__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__multigenus;s__multispecies_spp234_5
	   
	   The above example indicates certain reads match equally to 5 different species, which belong to multiple genera.; 
	   the “spp234” is a temporally assigned species ID.
	
	4) Unique level identified – unknown species, potential novel species:
	   
	   k__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__Roseburia;s__ hominis_nov_97%
	   
	   The above example indicates that some reads have no match to any of the reference sequences with 
	   sequence identity ≥ 98% and percent coverage (alignment length)  ≥ 98% as well. However this groups 
	   of reads (actually the representative read from a de novo  OTU) has 96% percent identity to 
	   Roseburia hominis, thus this is a potential novel species, closest to Roseburia hominis. 
	   (But they are not the same species).
	
	5) Multiple level identified – unknown species, potential novel species:
	   k__Bacteria;p__Firmicutes;c__Clostridia;o__Clostridiales;f__Lachnospiraceae;g__Roseburia;s__ multispecies_sppn123_3_nov_96%
	
	   The above example indicates that some reads have no match to any of the reference sequences 
	   with sequence identity ≥ 98% and percent coverage (alignment length)  ≥ 98% as well. 
	   However this groups of reads (actually the representative read from a de novo  OTU) 
	   has 96% percent identity equally to 3 species in Roseburia. Thus this is no single 
	   closest species, instead this group of reads match equally to multiple species at 96%. 
	   Since they have passed chimera check so they represent a novel species. “sppn123” is a 
	   temporary ID for this potential novel species.

 
4. The taxonomy assignment algorithm is illustrated in this flow char below:
 
 
 
 

Read Taxonomy Assignment - Result Summary *

CodeCategoryMPC=0% (>=1 read)MPC=0.01%(>=2301 reads)
ATotal reads24,112,01124,112,011
BTotal assigned reads23,017,74923,017,749
CAssigned reads in species with read count < MPC0628,579
DAssigned reads in samples with read count < 50000
ETotal samples240240
FSamples with reads >= 500240240
GSamples with reads < 50000
HTotal assigned reads used for analysis (B-C-D)23,017,74922,389,170
IReads assigned to single species10,121,8529,994,063
JReads assigned to multiple species320,041310,693
KReads assigned to novel species12,575,85612,084,414
LTotal number of species3,349515
MNumber of single species753152
NNumber of multi-species607
ONumber of novel species2,536356
PTotal unassigned reads1,094,2621,094,262
QChimeric reads124,900124,900
RReads without BLASTN hits23,29223,292
SOthers: short, low quality, singletons, etc.946,070946,070
A=B+P=C+D+H+Q+R+S
E=F+G
B=C+D+H
H=I+J+K
L=M+N+O
P=Q+R+S
* MPC = Minimal percent (of all assigned reads) read count per species, species with read count < MPC were removed.
* Samples with reads < 500 were removed from downstream analyses.
* The assignment result from MPC=0.1% was used in the downstream analyses.
 
 
 

Read Taxonomy Assignment - ASV Species-Level Read Counts Table

This table shows the read counts for each sample (columns) and each species identified based on the ASV sequences. The downstream analyses were based on this table.
SPIDTaxonomyF20139.S001F20139.S002F20139.S003F20139.S004F20139.S005F20139.S006F20139.S007F20139.S008F20139.S009F20139.S010F20139.S011F20139.S012F20139.S013F20139.S014F20139.S015F20139.S016F20139.S017F20139.S018F20139.S019F20139.S020F20139.S021F20139.S022F20139.S023F20139.S024F20139.S025F20139.S026F20139.S027F20139.S028F20139.S029F20139.S030F20139.S031F20139.S032F20139.S033F20139.S034F20139.S035F20139.S036F20139.S037F20139.S038F20139.S039F20139.S040F20139.S041F20139.S042F20139.S043F20139.S044F20139.S045F20139.S046F20139.S047F20139.S048F20139.S049F20139.S050F20139.S051F20139.S052F20139.S053F20139.S054F20139.S055F20139.S056F20139.S057F20139.S058F20139.S059F20139.S060F20139.S061F20139.S062F20139.S063F20139.S064F20139.S065F20139.S066F20139.S067F20139.S068F20139.S069F20139.S070F20139.S071F20139.S072F20139.S073F20139.S074F20139.S075F20139.S076F20139.S077F20139.S078F20139.S079F20139.S080F20139.S081F20139.S082F20139.S083F20139.S084F20139.S085F20139.S086F20139.S087F20139.S088F20139.S089F20139.S090F20139.S091F20139.S092F20139.S093F20139.S094F20139.S095F20139.S096F20139.S097F20139.S098F20139.S099F20139.S100F20139.S101F20139.S102F20139.S103F20139.S104F20139.S105F20139.S106F20139.S107F20139.S108F20139.S109F20139.S110F20139.S111F20139.S112F20139.S113F20139.S114F20139.S115F20139.S116F20139.S117F20139.S118F20139.S119F20139.S120F20139.S121F20139.S122F20139.S123F20139.S124F20139.S125F20139.S126F20139.S127F20139.S128F20139.S129F20139.S130F20139.S131F20139.S132F20139.S133F20139.S134F20139.S135F20139.S136F20139.S137F20139.S138F20139.S139F20139.S140F20139.S141F20139.S142F20139.S143F20139.S144F20139.S145F20139.S146F20139.S147F20139.S148F20139.S149F20139.S150F20139.S151F20139.S152F20139.S153F20139.S154F20139.S155F20139.S156F20139.S157F20139.S158F20139.S159F20139.S160F20139.S161F20139.S162F20139.S163F20139.S164F20139.S165F20139.S166F20139.S167F20139.S168F20139.S169F20139.S170F20139.S171F20139.S172F20139.S173F20139.S174F20139.S175F20139.S176F20139.S177F20139.S178F20139.S179F20139.S180F20139.S181F20139.S182F20139.S183F20139.S184F20139.S185F20139.S186F20139.S187F20139.S188F20139.S189F20139.S190F20139.S191F20139.S192F20139.S193F20139.S194F20139.S195F20139.S196F20139.S197F20139.S198F20139.S199F20139.S200F20139.S201F20139.S202F20139.S203F20139.S204F20139.S205F20139.S206F20139.S207F20139.S208F20139.S209F20139.S210F20139.S211F20139.S212F20139.S213F20139.S214F20139.S215F20139.S216F20139.S217F20139.S218F20139.S219F20139.S220F20139.S221F20139.S222F20139.S223F20139.S224F20139.S225F20139.S226F20139.S227F20139.S228F20139.S229F20139.S230F20139.S231F20139.S232F20139.S233F20139.S234F20139.S235F20139.S236F20139.S237F20139.S238F20139.S239F20139.S240
SP10Bacteria;Firmicutes;Bacilli;Lactobacillales;Lactobacillaceae;Limosilactobacillus;reuteri15000000000000000000000000019000000000000000000000000000000000006000000000000000005400000000000000000000003000000000000000000000000000000000000004000000000000000000011084240024000071151043720002310561451160805125040000041422603311308114110937297293138627631555407200143357462361792096465681664711423551129012790248160001517851727746524184455113053726968429
SP100Bacteria;Fusobacteria;Fusobacteriia;Fusobacteriales;Fusobacteriaceae;Fusobacterium;nucleatum_subsp._vincentii00000000904100750000001003900210000007218002900000010098002241001005005606108980091300978004843937900106900298005143776100147200974006174797600540200825005075775500256100113200740347400111200202300207228481204810064700167308559008930021700169448943920505003870061546071104014590017800420455371784074500970016304649367021000000184000850053600202942070040000150002661678490166001260011582417160000000000000000000000000000000000000000
SP106Bacteria;Actinobacteria;Actinomycetia;Micrococcales;Micrococcaceae;Micrococcus;aloeverae00000001000000000000520000023000400000000138000253156237087000002717000180000000060380001241663600018300159740000001170000000760079000000000900000001380007000000088270000000000000000001360016000000000000000000000000000000000601120000000000000000000000000000000000000000000000000000
SP108Bacteria;Firmicutes;Negativicutes;Veillonellales;Veillonellaceae;Veillonella;sp. HMT78000000000000000000000000003100000000090000000200050000000000000000000800000002042000000000000000000000000000300000000005000000000000000000000000000200000000000000000006213527813369000006570203235240000040526020200000020630527000000000000000000000000000000000000000000
SP110Bacteria;Proteobacteria;Gammaproteobacteria;Pasteurellales;Pasteurellaceae;Aggregatibacter;actinomycetemcomitans347836327079011174207811031183160373528836016122054237105013135819150661637908236349587604721168407290596126953598314218231283226107501724233671904512352909587014390154285116969335586023833627202165598190300237271696289401275511131059427610022184656510454472185246303881127202164800012108111900326300978513061220120801700394593192720501600841054496731491385717955100449840762738233511346215122379200213560733320527867691289785157911701596219821237734600394672635499138567152509801322454655187517123335860922720511309534471054842103452562615827364469128496846917325958349847211618681954230000000000000000000000000000000000000000
SP113Bacteria;Proteobacteria;Alphaproteobacteria;Sphingomonadales;Sphingomonadaceae;Sphingomonas;hankookensis000000021000007400000000000850110000000006100112014271613711122900242391511172096325241300070395503801451036200027601338846151400074030000000604294000001907388000000001458000000016123000000808150126000000900300001200000714000000000000000030000000000000000000000000000000000000000000000000000000000000000000000
SP114Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Coprococcus;eutactus0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000029000000000000003018287931961432769027885945282521020008442111212592380170213546036238531331040
SP120Bacteria;Firmicutes;Clostridia;Eubacteriales;Peptostreptococcaceae;Mogibacterium;timidum006220000140000043800003410000300000200000390000023700001600001102400455000000000211000030000198000000000671000000670021000038000011000013200001200000000094000000000600000000070000163000070000000007000000000000000000500000630000000000000000000000000000000000000000000000000000
SP131Bacteria;Actinobacteria;Actinomycetia;Micrococcales;Micrococcaceae;Micrococcus;flavus78426110020005000400850543204517002926001821280002194524148094004020076581008000001201600004700519561700314290228852800142660003801800034603100000521012000001100000002636300013723057000031005381005000698000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
SP132Bacteria;Firmicutes;Bacilli;Lactobacillales;Lactobacillaceae;Limosilactobacillus;mucosae000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000003000000000000000290167001412004053262717409900000026541214210428321129006766443500024000
SP133Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Lachnospira;eligens0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000015111500000000000088273111101679711240215601343122198592412700030752017274712122024430271623837304
SP14Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Blautia;luti40000000000000000000000000000000000000000000000000000000000000000030000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000003000000000278000000000000040242164197209333303657616910301817625523014330284216241214233240319519818373105639435136518312712220855
SP140Bacteria;Firmicutes;Negativicutes;Selenomonadales;Selenomonadaceae;Selenomonas;sp. HMT4420000000000000000000000000000000000000000000080000450000170000253000015000000000800003990000700008670000730000629000000000612000000000930002200000011400001300401390036000000820047000000135000000000000000000000000200000600000000000000000000000000000000000000000000000000
SP144Bacteria;Proteobacteria;Gammaproteobacteria;Pasteurellales;Pasteurellaceae;Aggregatibacter;sp. HMT898000000000000000000012500000000047000000000150000281700000000012630000330000384000077500004590000000001782000000000244000038003720013900000002115018600000000041000610000000710022790970087031001590000108802730000000000000062000056000000047902000001100155044600001170000000000000000000000000000000000000000
SP145Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Catonella;morbi090100000012941002012280000006627650136600000012451490209000000160213007870091300012283310277003970004488552011020026600058593501620050300035521020100900189500040613880927008450001336420076036093400014613606030014720007453590118011380699002453921823048024906870016331422622090131707810023572917960826254066900232320578108200000011840303700160009200191001917000002865206000181160000000000000000000000000000000000000000
SP146Bacteria;Actinobacteria;Actinomycetia;Corynebacteriales;Corynebacteriaceae;Corynebacterium;casei000000000002000000000000000000000000000001800000000012000000000810000000002500000000000000000000000000000000000000040000000000000000000000000000000000000000000000000150000000062267003000000029900600450404900000000000011000000000000000000000000001100000000
SP148Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Alloprevotella;sp. HMT91400000000000000000000000000000020000000000000103900000000070800000000062900000000056100000000028800000000070400000000093200000000020000000000812000000000435000000000275900000000033640000000000000000000130000000001300000400042800000000000000000000000000000000000000000000
SP150Bacteria;Proteobacteria;Betaproteobacteria;Burkholderiales;Burkholderiaceae;Lautropia;mirabilis43748000016903046132000060000121880000000516420013970206802215700000005728210000000382540010840003600000600008310004853000165055000318800073033000240701720000400001220210004160026315300611300026018100529000877590002300035482001026000001230311161200003905160400031101320260065000000000000000000000000000000000000000000
SP152Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Tannerellaceae;Tannerella;sp. HMT80808300000008394990226500000027787102825000000031404480000000260825900002190004095629300249000201542000000070246360000007019483190065000150219200014000130145876000000240212317000000501462731000000801876540000007101642326000000228017361950000001107525100000111800663930000000051069012005400005061500000000000000000000000000000000000000000000000
SP154Bacteria;Firmicutes;Tissierellia;Tissierellales;Peptoniphilaceae;Parvimonas;sp. HMT11001281000102605726004078966220052460000279201180012798000527821208004009000071200010520000377750007350000300502580046013350001988591650014090000146100004100000176000015930000481030075430000662723400310300029800037015590000186900824203400025270010002273420008661050701556001801172014271500721141211068157350024149007425067236921555314000000000000000000000000000000000000000000
SP16Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;stercorea00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000013000000000000042803595357419495846822265645072502641533556220283934114653633816245063018213119115445696013922439177
SP162Bacteria;Actinobacteria;Actinomycetia;Corynebacteriales;Corynebacteriaceae;Corynebacterium;xerosis1014006000000900009001500007076004010006017000011000000001600002900000600021000000000160000020000000013100030700002000000002100000094000300000000000003300002000000000000000002199120373027777250374283512241509302611924433866422264694554572881704003719000006190000920001103900001701717000950400542940
SP163Bacteria;Firmicutes;Bacilli;Lactobacillales;Lactobacillaceae;Lactobacillus;amylovorus0000000000000000000000000000000000000000000000000000000000000000000000000000000047000000000000000000000000000000000000000000000000000000000000000000000000000000000001000002900000000000000019293840018000100500470109953495942678005405230105715581265901426665578216974289571263238421250977197785801470223960140
SP169Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Bacteroidales_[F-2];Bacteroidales_[G-2];bacterium HMT274066465620580051102190995445003510076404020107471200004909806923630126001988031262358570730011100344696646036006601146787696640054900210031713437605700003403287579000069203010298118025100338053122452150000776033228326000000048906064680292002200337015019603050043004548301927610335001599010808781970129003040122001107710000175170500000001831812000000020478000000000000000000000000000000000000000000000000
SP170Bacteria;Firmicutes;Clostridia;Eubacteriales;Peptostreptococcaceae;Intestinibacter;bartlettii00000000000000000000000000900000000000000000000000000000000000000000000000000000000050000000000000000000000000000000000000000000000000000000000000000000000000000000508800000019800070000067021090190000000035538054281110461297923290529512421889686210462868316873112450957179968686112186022357626516031973039742257134112663202374166
SP171Bacteria;Fusobacteria;Fusobacteriia;Fusobacteriales;Fusobacteriaceae;Fusobacterium;hwasookii3008227448927801401803587772392754813120832133956165118401223540454793534535150921433897225214701183753621788506801843045799842471076117371235210075033672827622159316801099223346965210529470112134215109342018273107627957276125761592569115710281296986568860840211961531236911743471704645123606162064741401663305976936752311817876082071631231490523374229172139816031915148254322891799356542831557034013670463270250514728258554714553964441955023332554547207419871683181188730852047609186437523762271105664021474670103218401727196896624947338796511764311198161718086773847588038883694081179529731264591869611068530228813192324601332114951237750289205577318160336103522662116114825910172004210000000000000000000000000000000000000000
SP174Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Lachnospiraceae_[G-2];bacterium HMT088818494246720181404676718803515503713215321877284940362173839924432664588523803392933137506713714974443781638061784535752017241303741409530261829179151211291211273886054419419313153324680224663668559925364216220494921367783667381531180519001832389594255440103417023320125381544376023119564191284812132807932532040886834350185082562613244620331021992883639132437455656022771141210719382604390273051170412604812842161559521422241671555939339582189743476902230386564636835331496265531211533232039931883243814273697372374635464883248163627973003230669634932520405510582146261081776536169221076394312031663474806019676471038639794446136017194216635016015298873794475137139438130914291910000000000000000000000000000000000000000
SP177Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Coprococcus;comes0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000002200000500000007879167371092162147815501158983847915915323766656464174119001471411241331001138065791941297021353
SP18Bacteria;Firmicutes;Bacilli;Lactobacillales;Carnobacteriaceae;Granulicatella;adiacens067830000000600515000500001610608000000021020000000550113300000006244747840002000370231200000002460120000700023509100140400850102000021004204480005600000333002501700210380000000037490000000310215000180002001000007000433921150701522733515678972604921626145231263237810469348303920336622231313521285516972170000000000000000000000000000000000000000
SP180Bacteria;Synergistetes;Synergistia;Synergistales;Synergistaceae;Fretibacterium;fastidiosum0000000001390000000002410000000001840000000003890000670000106000022000029300004250000800003900002220000150000137600001440000132800002000005570000260000294000013600570488000014100390369000033100502015100002780040003420000000006400000000000000230000000000000000000000000000000000000000000000000000000
SP183Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Alloprevotella;sp. HMT9130359168956241400691601092478048880659545103555610189150501519116299789004400151502964924030520553236202065803100472015710202214295365181971770212753876853019042107424191797047000152085971678745484082232350225636109944011460176814801270668191221000435023655311059128000112870401353610791011304915030102819430624707502583530314181841981558075032519444441950119300406013354727215353131994016731501436510615523930006380090412556229732406011157742352470004301225287021581667380103830000000000000000000000000000000000000000
SP187Bacteria;Tenericutes;Mollicutes;Mycoplasmatales;Mycoplasmataceae;Mycoplasma;salivarium000000097162000008009405400000000240000000001940000000000000000000019400000000593100000000000000000017000000000090000000085100000000000000000000000000000000000000034500000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
SP190Bacteria;Spirochaetes;Spirochaetia;Spirochaetales;Treponemataceae;Treponema;socranskii0000000001910000000001770000000004400000000004140000530000213000050000415000021000000000015900003060000320000141300001070000116200001130000686000090000400000045000057900003500005950000191000024900006300003070000000003100000000000000110000600000000000000000000000000000000000000000000000000
SP191Bacteria;Actinobacteria;Coriobacteriia;Coriobacteriales;Coriobacteriaceae;Collinsella;aerofaciens000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000030000000000000000000000000000000000000000000000000000000000000000000000006000000260000800000000671236915188297220826678731451402032452756715234980493213904131842281588932932830663846635230656012681611473
SP192Bacteria;Firmicutes;Clostridia;Eubacteriales;Oscillospiraceae;Faecalibacterium;prausnitzii00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000001504000000000007000043040000000000000361111539276142516445293511466041287729028541536289277278489623152801421439617688511601034010815946429914751531
SP2Bacteria;Firmicutes;Bacilli;Bacillales;Staphylococcaceae;Staphylococcus;warneri23000000000000000000000200026005040000000000000030010000000000000000000011000000000000000000001500000060149000009077000000000170000000000070000000000000000163000000000022621700110128763110538005654471428194107362922142954836631591414114922121522105260340000003000000000003000000000002000000000
SP205Bacteria;Proteobacteria;Gammaproteobacteria;Cardiobacteriales;Cardiobacteriaceae;Cardiobacterium;hominis1572217006319812156103858191001029134816505847141323908371510233117234880240330161343608648470134114932094415069910972113927977220291771401041567173909430800140090270804605155621026670016520114132166058236020995120427410354320185908026002400015308631134021032140953912640739108695103158260563710145200157658018800156197007142940022810150100435034446800000992030091138000120000000000000000000000000000000000000000
SP206Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Porphyromonadaceae;Porphyromonas;pasteri3920286100989599374131801345310926054702243810610192727033485199681931711714068530327310816290114851253025017524515788818698273322956917400749591912443212522441115412119203304192468341450796258021112184885072976751871639001952165212344726290885721228600237577114431671765620736121074300383908348280591124625065182901292480204298671252325453183309115675151783139662132522439291702396817176581381534119931238631317202144596100643814441724207836099914631496761643185225730522793105048421591038227325542199100017523962642345438265055165632951196170221294826225877814441034113022089113433721016516477196432504917106423862704126563877602964446920650000000000000000000000000000000000000000
SP207Bacteria;Proteobacteria;Betaproteobacteria;Neisseriales;Neisseriaceae;Neisseria;cinerea00000000000000000000000043000000000200000000002100000000031000000000170000000001900000000046000000000700000000000000000000000000000000000000033000000000000000000000000000007710000000003170000000000628200000000099000000000000000000000000000000000000000000000
SP21Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;copri000000000000000000000000004500000000000000000000000000000000000000000000000000000000020000000000000000000000000000000000000000000000000000000000000000000000000000000001000000003739100022000001232292250100715000000029205879330515351513181317778033363551019281148709337625505029234650710132322890399619925296958728506101932958937193754021931072209291279124231457
SP210Bacteria;Firmicutes;Erysipelotrichia;Erysipelotrichales;Erysipelotrichaceae;Holdemanella;biformis0000000000000000000000000000000000000000000000000000000000000000000000000000000000000060000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000016035000000000000103201139145111270263784041219311311823689248231369274861659664293263021317627682125714115545911848549258933699
SP212Bacteria;Actinobacteria;Actinomycetia;Micrococcales;Micrococcaceae;Kocuria;carniphila18000000000000050000000000512020000005024000000000000000000004000700000000000000000000000000800000080006090000000000050000000000000000000000000000000000100000000000064197260411938131269241335960769161955625821064959160465571910227177841583013160177330000000090110300003001900000040000700005018160
SP213Bacteria;Fibrobacteres;Fibrobacteria;Fibrobacterales;Fibrobacteraceae;Fibrobacter;intestinalis000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000300000000000000000000000000011110070170013650185093002440117146115726202904003597275384086815235
SP215Bacteria;Spirochaetes;Spirochaetia;Spirochaetales;Treponemataceae;Treponema;maltophilum0000000001450000000001020000000001010000000005100008100007500002300002140000200000000000620000124000015000079600001920000546000040000033500000000026000006700003290000440000350000017800001910000960000299000000000160000600004000000000000000000000000000000000000000000000000000000000000
SP216Bacteria;Saccharibacteria_(TM7);Saccharibacteria_(TM7)_[C-1];Saccharibacteria_(TM7)_[O-1];Saccharibacteria_(TM7)_[F-1];Saccharibacteria_(TM7)_[G-8];bacterium HMT9550490000001080008550000034300023500000000052000006000860000018400025900000920001770000000006800000000003470000052200079100000249000620000010000970000000001060000023200012800000265000353000005400095000000000220000000004200000500010300000000085000000000000000000000000000000000000000000000000
SP218Bacteria;Proteobacteria;Epsilonproteobacteria;Campylobacterales;Campylobacteraceae;Campylobacter;showae0936731497333162622971111979216285681364477163160275118580226282437004481140399171899261244701309232085272246411536255591255976111293281141101407065202595224581183031618131918216891636319301567332407276142664620750152973710107154814010724638611518850056417425026571055232121339012070532159971864756267879152400567595795199369530881927611004084776710860300866230867141768128784707896306962239304379916801931106465955766932501154516721150109150147461728798184013215740302016188000004621840364541831203116200324640901281100051350234116614350054126340000000000000000000000000000000000000000
SP219Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Tannerellaceae;Tannerella;serpentiformis00014685100255007100397153006364020008463600400011500406215001100000721566700000005275800032013630010083100041011700100474008500002469204060670024800235184106700706530026766700600122000147670056000001356027814927000002181059139320088500238039190001622007030291486000100044554500120070019968004690150018547166024001500186295387312000000000000000000000000000000000000000000
SP22Bacteria;Firmicutes;Negativicutes;Selenomonadales;Selenomonadaceae;Veillonellaceae_[G-1];bacterium HMT1350000104000087000011600008200001100000000019000013000021700280530000219000011400004500000000005190000240000036009604390000271003502630000770016203040000130030387000031300002080000318000016000005740025044000035200159024500003300009200001200000000000000000004000000000000000000000000000000000000000000000
SP225Bacteria;Firmicutes;Clostridia;Eubacteriales;Clostridiaceae;Sarcina;ventriculi00000000000000000000000000000000000000000000000000000000000000000000300000000000185000000000000000000000000000000000000000000000000000000000000000000000000000000001000002000000000000014000000294701209000000007654794374999611891375242785139274998317751163974361427128251618411154931642163545021507421529463988597164433362213125511170
SP23Bacteria;Firmicutes;Clostridia;Eubacteriales;Clostridiaceae;Clostridium;saudiense2000000000000000000000000000000000000000000300000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000005000300012033004114270006800000004958411145385110435053429616098018723118402312451133337020183295802892952589233139253341177
SP232Bacteria;Firmicutes;Bacilli;Bacillales;Gemellaceae;Gemella;haemolysans836335044865781229000820361763343935106751360821731210113304880067002365821697984365721802422115228096160712590611192657296560108811298163145770480113510576725731311659702450322244253027001305208100976060016062001010727527512352575200500017069150105611591897029250153116350790602584000824015922616120003134640174713696259112330430109958199081139811618511903387807535207010724443333551133435015034234419272068271537856691166230923573675181317459906878577874518400093140735308584624812222106540000000000000000000000000000000000000000
SP233Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Porphyromonadaceae;Porphyromonas;gingivalis0000000004210000000007640000000004000000000000006336000050500004218000097400006987000010000034850000671000020700006273000080030000299000001350000343800000000011730000108530000213500009456000014200000888000002810000917900001027000000000205000032000027000079000026000030000060000000000000000000000000000000000000000
SP237Bacteria;Saccharibacteria_(TM7);Saccharibacteria_(TM7)_[C-1];Saccharibacteria_(TM7)_[O-1];Saccharibacteria_(TM7)_[F-1];Saccharibacteria_(TM7)_[G-6];bacterium HMT87000000000000000000000000000000000000000000000000005013000000000165000170030014000120000400003600003000000080144000600001060008000016500000007170400000203920500000001914190000000001004275602700013835328130625340042105121951529018200190104320893179107211900460000000000000000000000000000000000000000
SP239Bacteria;Proteobacteria;Deltaproteobacteria;Desulfovibrionales;Desulfomicrobiaceae;Desulfomicrobium;orale0332001040066109790971003330011830100608250027600170015630147001490020060403541033380040907702570042000420870184806406550011200029210023001460001324783022610089301870116900915852003040338010690263380038140628024200105000184060474411671560183006620700191814513204003970437051605222222900242701380323225265191001694000430043008011805302190035000204060230130003671590000000000000000000000000000000000000000000000
SP240Bacteria;Proteobacteria;Gammaproteobacteria;Pasteurellales;Pasteurellaceae;Actinobacillus;lignieresii06140000080001840000009600538110000092000054900000239900460607007800250115000014001687049000062600074000144001340000104600360002963000070041312401100760041833010004600003801280133500903408501100012031013220071006512602704023800292448000001741004903412870160044290018836404660103416269572050664757701946010354600000000000000000000000000000000000000000
SP242Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;downii0381000000000207000000504129000000000260000001014010904860171179010001402052350380755280423100210199047006052402001103900410000001210017502900020000005011010025390000072201719000005180201000100035170013000190010150418327203000010378370114361419671028533518102061333029642028914201076216722171741119117222711940000000000000000000000000000000000000000
SP243Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;equinus0000000000000000000000000033000000000000000000000000000000000000022200000000020000000004000000000000000000000000000000000000000000000000000000000000020000000000003900012240161346004672562000090937912432201617027000180000303118514725777402017217929272486736892992992251464594823142190113218652661050509295168475027162271863274412801856621419015021390227075082424
SP247Bacteria;Firmicutes;Clostridia;Eubacteriales;Peptostreptococcaceae;Peptostreptococcus;stomatis019150033900000100701569003185000020208260012840000500001056000034012400274008606730162700148500138307100125050202450000401221070163001450160701100500350702423086700154006400468011701850500098802313069116003000130160301455023800660273901902306340070076401320669016100894031001374168014100548031920940050700006410840036006028037360061404470200363330310000120000000000000000000000000000000000000200
SP25Bacteria;Actinobacteria;Actinomycetia;Actinomycetales;Actinomycetaceae;Actinomyces;sp. HMT1711880377851857152229485750175814021210963102573206441923961685201110816636749227961556491363818012237414901505500341276357105621221154152628351322151331542324665527193125428212219402230162614387271460451602610125310116209272065317392691682221454000690109650349847714621222163263604071309836548434148554563212447000253913903869509550054396946709311643300511290263715205253143164674104520873806029201054913066951280113018313201129573184111044257591229296860670000000000000000000000000000000000000000
SP251Bacteria;Proteobacteria;Epsilonproteobacteria;Campylobacterales;Campylobacteraceae;Campylobacter;rectus08025112325200017794720407492235049892601198268500219318788017248011300051294134067003367300311010149530021983283035608531313367901256428841232134100084011081345828544090191286701003311795528720374171839143021893891182639520922193856055872831022110400079819208327958332490185933900481685303972066212761100118776932261098211672561430290793119239214480091314620531114672712296785021350021157956416830314521907260014709434160630613166000389001070334930011102212405151692300400340128488356500011700000000000000000000000000000000000000000
SP256Bacteria;Firmicutes;Bacilli;Lactobacillales;Aerococcaceae;Aerococcus;viridans017003200000018000000700000360000000402500210015370032000000000000000017000082000330119000390020860000006000000007900700000000000000000416000000004582000000001950070000003600000000052000170029505092460112908602000009434193412422716065858500917905400480000006000000000000000000036290020058000000000
SP259Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;periodonticum000000000730000000004500000000015500000000013000100801593736008550007880186558700730000004003680002040000033100011930177011800138000130101568460014800024000006000276000003700013720327392002100036440270170015000014000001070002480000043080804101801722016724433953400699011781029002470636120250000000000000000000000000000000000000000
SP26Bacteria;Synergistetes;Synergistia;Synergistales;Synergistaceae;Fretibacterium;sp. HMT36200000000019800000000037600000000068500000000037000000000156000000000110000080000000000000001710000000107010890000520000927000000043906060000000005400003400002650000390000234000055001680760000000440470000110000440000000000000000000000000000000000000000000000000000000000000000000000
SP260Bacteria;Actinobacteria;Actinomycetia;Actinomycetales;Actinomycetaceae;Actinomyces;sp. HMT169016300000000000000000000000000000523000000000834014700000001150397000000022000000000620000000000810130000000110000000000000000000100000000001701200150000551080000000000000000000000000003809000000057010300000006090130220000019901170000000000000000000000000000000000000000000000
SP263Bacteria;Fusobacteria;Fusobacteriia;Fusobacteriales;Leptotrichiaceae;Leptotrichia;sp. HMT2250000000001722000000000645600000000074200000000016803037910340000514013661780380000215041612110000041010106182400000464020087151150000840582381800000017601162501380000030058170334600005701379405173000001109734206722900000440213249412700000110011907446530000042000000000834049872039100006007016515468000020042812012845600000450000000000000000000000000000000000000000
SP278Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;mitis40811343162773831329112140018581744137180266395040822239162859183224481005647202914155977932338083514562801060102821281261592353286020288862434711315191981296262174577885582072711157151651881915601414299779172140711254859100611020080341361513120423200726240203039709029677715914679210910348403132554054225042903666966773166242196882201788586897024850555116131504800352135103140691372411061102938692525202929133232873101498285118491179148224831262293981559661246462741864150925691144078806494372171471960194017819781445012783128762774328419727091154262547705847224666645291137620000000000000000000000000000000000000000
SP279Bacteria;Actinobacteria;Actinomycetia;Micrococcales;Micrococcaceae;Kocuria;palustris140000000020001300000026000000800216000000201506009000301147100000301200000020019256000000015634006260000231900000270019000021000037000130016003056201600008068500000000518100000300000000004246160167046636215648804470761656494601143413980616617491196003100660000000000000000000000000000000000000002100
SP282Bacteria;Fusobacteria;Fusobacteriia;Fusobacteriales;Fusobacteriaceae;Fusobacterium;sp. HMT20402920000002930082800680003897501639003400000510126400152000012401075070000001160461100000012322304936002600000450733500880000170240530000009320115452390000041618050090034800000025680039500020051049000001880031567300000072000770200000060038030078000000328000000451780386190000080018810000000501038000000000000000000000000000000000000000000000000
SP285Bacteria;Absconditabacteria_(SR1);Absconditabacteria_(SR1)_[C-1];Absconditabacteria_(SR1)_[O-1];Absconditabacteria_(SR1)_[F-1];Absconditabacteria_(SR1)_[G-1];bacterium HMT345000146500053023200043395009044100036500000000014850000000170033572176000005740315115900004707190344330000003870458000002403230192541546042016601607011091928320002760004060000013907720547000001240752048998218000223000908101865000278010760489800009907150271084000219000337106500305100068470000000011405800000900591122471440000000000000000000000000000000000000000000
SP292Bacteria;Saccharibacteria_(TM7);Saccharibacteria_(TM7)_[C-1];Saccharibacteria_(TM7)_[O-1];Saccharibacteria_(TM7)_[F-1];Saccharibacteria_(TM7)_[G-1];bacterium HMT9570000000130000000003200000000000000000000000000219001913000202400151110000000000000000000000000110036200000700251535800000000000000000000002015003506900001000321116300007000052000016000046000000000000006000000000600000000001959000020000000000000000000000000000000000000000
SP294Bacteria;Absconditabacteria_(SR1);Absconditabacteria_(SR1)_[C-1];Absconditabacteria_(SR1)_[O-1];Absconditabacteria_(SR1)_[F-1];Absconditabacteria_(SR1)_[G-1];bacterium HMT874013423580000047050407552530000061022901086149000006274110288510000070410701561256222677775332050255303064941303095711000975906896232100429947045731402132212022245111150563255026213816837874300031015833821240112805008205114574591320039026044159213914265101890340226118434265604157358030702491044615073310929403050110835538388072613454515480109722279995135211283618607917110000052111503616000120674202942109119911538612552000704192262441113881240930000000000000000000000000000000000000000
SP295Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Catonella;sp. HMT1640019407950025510610018207890054860790069077900183502500800554001821013200007600043104070060105800409043232106701150002090014014708020017305420050014930012142895211101502256003962472780018015140061529511419900011130070781162342012801709003412667758027027460014572581214007901080029068317113201090586001395559599004010006501281500061009101490000000015144000400201000000000000000000000000000000000000000000
SP296Bacteria;Actinobacteria;Actinomycetia;Corynebacteriales;Corynebacteriaceae;Corynebacterium;matruchotii134906025530203648600041623334610124301326303600125639514090000420054687548303300000143212838690227302869255700846804024010000370158300491248927270000800137018161414043100000313082402422347715328007806498890511603939902006613500564090000001212764686013770000045426213012780625173708271035960429501041129001499271126975240000092225873710171000003086122015000002082527010702500008491075033900017013019132011000330000000000000000000000000000000000000000
SP30Bacteria;Fusobacteria;Fusobacteriia;Fusobacteriales;Leptotrichiaceae;Leptotrichia;sp. HMT39206105010422000003330910206367000001247025510304300000276012840598200000122064552512877237000210251604569619684150004951280216210841752500066042505731241611137000284925050520442626000016066407184241446486001110611700426413234942330001329360538562334739200223987012390243730200441298103013220165295007585730801552171603000167268890109680293779001681008121060037300000288013711942001675000279800770669330510140041740014487306395900489558800000000000000000000000000000000000000000
SP308Bacteria;Fusobacteria;Fusobacteriia;Fusobacteriales;Fusobacteriaceae;Fusobacterium;periodonticum00004089000258152600001636000329929000057790000286000092530005160225164475082800131814224170533716301917006108267351871119664502600036152508543372322402066003666221102921054500009351156355980185000351487541232741797706000005029138177320874528000129115110381466529658001545151157537206776762426070024765823955189142641092201295153142891040565804905365615821001311310960214100004501000680269322843456091300723262981382675352755450014212329187080041179192301274214200000000000000000000000000000000000000000
SP310Bacteria;Absconditabacteria_(SR1);Absconditabacteria_(SR1)_[C-1];Absconditabacteria_(SR1)_[O-1];Absconditabacteria_(SR1)_[F-1];Absconditabacteria_(SR1)_[G-1];bacterium HMT87500021755870000000012435900000000012440000000031339000000130396304002840002027856300672000481091043300770006551227023700128000007213600038700000444328004060000032133700780008292037080097300022020167763700903000276019937150035300014074084341005780001269358385750050200039106456010000590560162301366003630320618218516600420324403901115159440033401500000000000000000000000000000000000000000
SP322Bacteria;Actinobacteria;Actinomycetia;Corynebacteriales;Corynebacteriaceae;Corynebacterium;suicordis323200110000801514000000630000065000171300701605422700000000012000000000150000050411000000000000019004006018003014601445000056420018800000501440020000000000000000030000000250000200040040188270311331341181249915501223257351763148227274351375913115022872079423348290155944016227600006000003000000100050150168001094130000089100
SP323Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Johnsonella;ignava000012000016900000000076400000000095400000000036450000124700159004350000228009330260000974008490340000364003430630000133000128032153000044004472014400003860067002030000619001184077000060001818215037000013005777768102000016000144010657000020600201302950000260000204000017500710000001270000100000118008060000000000000000000000000000000000000000
SP346Bacteria;Proteobacteria;Deltaproteobacteria;Desulfobacterales;Desulfobulbaceae;Desulfobulbus;sp. HMT041000143344237044918100010567000182830008881100001122000214143000026820001983191132000034400018856060027444600088069500007000521257000046800099045864041511530001748669020313611053000126945000047000015015800006220002143915470434424000001247041083266500014217000005960004143400006400001352000076000000000000015100000000000000600000000000000000000000000000000000000000
SP358Bacteria;Firmicutes;Bacilli;Lactobacillales;Aerococcaceae;Abiotrophia;defectiva052901650015576000490455205280000910134702020600064100110011151003400312152403325250400012941235002370030001060444300720055907449000001163372000000600000000128473853700000754801628300000310915227000005104435300000092202400000015250200003901810005100000482550082000001559392819100000011500191000000000000000000000000000000000000000000
SP36Bacteria;Firmicutes;Bacilli;Lactobacillales;Lactobacillaceae;Ligilactobacillus;animalis00000000000000009000000000690000000009000000000000000000000000000003000000000000000000090000000000000000200900000000600000000000000000000000000000000000000000000003001610102300000523648048006091874921393232013000190000939715565414279574060142724957214737061176353544263248110169260888765213481352431170212901474604124228771447132916745596992965487360957885905849
SP360Bacteria;Proteobacteria;Gammaproteobacteria;Pseudomonadales;Moraxellaceae;Psychrobacter;sanguinis6150000000000000000000000009500000000018000000000002530000030004000002000210000005000000060400050000000000000005011000050007120300000000000000000000000000020000000000004393321516270373286237181980332766469526035218318816351873993112824262503144529515452219581590000005000000000005000001213000002300000000
SP361Bacteria;Spirochaetes;Spirochaetia;Spirochaetales;Treponemataceae;Treponema;denticola000071400001750000108700001260000742000090000579000049000075600170016100003580046904310000311900128000000109800700111800001590014080904000018440059908970000388003423077500001120080069400001320033683861000008400239851289000094900186059320400007550018321788250000506000062000038005000000042000060000110009420000000000000000000000000000000000000000
SP375Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Fusicatenibacter;saccharivorans000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000711900000000000315536351695311031162530135973498922020324021233123800145247302126980204143808118
SP392Bacteria;Proteobacteria;Betaproteobacteria;Neisseriales;Neisseriaceae;Neisseria;oralis184100003792897600658000066575450017500004858005266000079197000110570016203493900618100049258720230018111005003560022253910011021178004154005116112100500019250000592308800181430021508110810001620010604691270002780007015320002570000014680003700250052000407016180022000631001332166900260139074136382145328006800461046620754293881118043643447821026150480000000000000000000000000000000000000000
SP398Bacteria;Actinobacteria;Actinomycetia;Micrococcales;Micrococcaceae;Rothia;endophytica573300130000290210060160022000011012300140000083000207000184000000000500000040060000000000260000000021000000000014240001400009100900000000001500000000000000001500530130000017000000000002183161021115021861621765532228917074219216212924388453125153141121738207225792163462385952711815213625214403800007700000000000010006210010011350201900000000
SP40Bacteria;Actinobacteria;Actinomycetia;Propionibacteriales;Propionibacteriaceae;Arachnia;propionica16659269091174827589366011874331353629293522851956157139405153103831911718105516051645308920362431374216291881352762130823861384221391515129913707485558187516709562551238719591309159127578381921477254047557801890451822253710523349322819013192248534176783305019493099462433871059262434454785391611821901941039001091143832481847238717694803148730989231737282149740021019216430141643855712645228365640341282510514270248531009912916711746401834951500114274537181530311156908730157851526302388962315825825681556262790578279830020954513552277237502031648014069411697309533071492422184461852612187193723992882578927875528206081473971304020842784802104531603617641002003260558789114370116878110522015223559762751791289661481262531690003000000000000000000000000000000000000
SP415Bacteria;Firmicutes;Clostridia;Eubacteriales;Oscillospiraceae;Ruminococcus;champanellensis000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000030000000000100000000000001105305464455304617222900618110029514670000860292036468917001172016119
SP419Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Porphyromonadaceae;Porphyromonas;sp. HMT277001411537500032300000173280480512455366200190934166431215311369121000207268102436034300000198139806945230409100038565200277001435998000110592833900803711200013887107189200020400002200116000102810000884912600142813460007721411400297600002648879002290000021781900016714300036280107400251200051628150029918100014055360000304300717162502650461110391412245814701112160082026202101042301701535000384800000000000000000000000000000000000000000
SP42Archaea;Euryarchaeota;Methanobacteria;Methanobacteriales;Methanobacteriaceae;Methanobrevibacter;smithii000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000020650000000003000001910000000000000073063417901961020954768126848034340284325324230401106065365891218192141319726469443197183510001541194116428012652250
SP425Bacteria;Firmicutes;Bacilli;Lactobacillales;Lactobacillaceae;Lactobacillus;johnsonii4000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000300000000000000000000000000000000000000000000500104000000002203051035604000038000001100484050011421429240209431144609646215190695795105561472262241775156642234926921695731113604405084046259062991463014395
SP431Bacteria;Fusobacteria;Fusobacteriia;Fusobacteriales;Leptotrichiaceae;Pseudoleptotrichia;goodfellowii00003000000000070000029033302650001209076209500170015049600193000020020500474000094097000011004908902312000000201001000000061000000015012090090000507700000006700190000180220000000000000038000057050000012014000010200180004190000100001400000000000013000000000000000000000000000000000000000000
SP434Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;sp. HMT31701963508049870964119812683790459042581605169347244950210928117501591065813310013114528460172130116456801882152863928406712436104481352151039192154805280219571965151225222969571423093381114118014942167954942704452055633982080681762160061515870104224036520713102005016471110301163780212011239118818686101801575980172126169645333333114344044101956463102104552080213685192665504484174920134751436844609496423201705752201531401400007281004849908501566180073210022030000222000000000000000000000000000000000000000000
SP438Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;thoraltensis00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000002500000000000000000000000000000018100036821215209400005461441080500017207077050001088380849050000000000000000000000000000000000000000
SP441Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;sp. HMT423060000040222000031028015587000210011301745440001650023101783340000563055402810770000760212007350000650640442275000064010602635500000007900000015450140498000076021108513600012810503014022000003609800280000004050048000000288009100011900417006400041271005290825187401060886000104505133621500064375065011437002040121695897803796078174301370000000000000000000000000000000000000000
SP443Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;sinensis0000000000000000000000000000000000000003000017780000157000010860000211000015400002000050000073700000000010200001870000118000032000023000005600001677000017650000162000017290000400001248000030000301400006230000000000000093000010000014000011000082000030000000000000000000000000000000000000000
SP445Bacteria;Actinobacteria;Actinomycetia;Actinomycetales;Actinomycetaceae;Actinomyces;sp. HMT17017001020270025100098158865023821179600010001810040160005440400190000626119525302616936700097135217040118211133200580113804439500060891004397000045110826061095124941200116323585502426633714842705617631101264244700078101102022754030070274826505540255106262028713802110141899304274720318341902635723000650477178613200007400006000073020740000070006001490001111038170000410000000000000000000000000000000000000000
SP45Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Lachnoanaerobaculum;sp. HMT083000059500003611000093400005483000037100001513000058500005160000133001250123700006900807280000484000061000036400508360072015800100702147000096600170211000142703830019301273003230450028301926038325890443002930960200729050100233050802775771018210045204780438385106240052409470000105400009250000220000170013703600009023370129000000000000000000000000000000000000000000000
SP459Bacteria;Firmicutes;Clostridia;Eubacteriales;Peptostreptococcaceae;Terrisporobacter;mayombei000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000040000000000000952081142201141652271837210001080730006008961756848225850867005715312047331170
SP460Bacteria;Proteobacteria;Gammaproteobacteria;Cardiobacteriales;Cardiobacteriaceae;Cardiobacterium;valvarum00206000000440012000000043100810000006520024400000013300504400301000013001191590100000035097620430000196016252003600003401816802770002436008326025000083010658805700001620219160150000590541906800033600574510600145171350814500140003148027677018000093000000000360132107400000011558033000000420083000040000000000000000000000000000000000000000
SP47Bacteria;Fusobacteria;Fusobacteriia;Fusobacteriales;Fusobacteriaceae;Fusobacterium;nucleatum10562295976871247980879370143451413958851100042861281155735448882211093154151337971781215914407259801812953010665868169121751677244876174331027130051167813544868510797132067585388120211485923828190061236714257113166124888852025368975110251323898531463472906147725636878154187865001142984646119391871386911158045574764829447861377095262085225635197736730860958747281938114769116893080458796103899272160061037816810564214067372382064864164330462190784291206610843375538127477413417581832201651487712286709571694813112438576224116346442539816609428414869156911089451163127841131963283186894556131291800613684432547358274581260381333044681728120953753561458211667210039421914512350812146301553669795333863202521612599663297763474243423377848702779499124776242810893198020934259818223812203410875534545034226455429462985922129828805076286415411378990292005368913200000000000000000000090000000000000000000
SP475Bacteria;Actinobacteria;Actinomycetia;Actinomycetales;Actinomycetaceae;Actinomyces;viscosus0000000000000000000030000002206290050003503300000003000000000000600030000037223100210008060000000000000000000028200700070531200470001100000000000000000000007700110000014127000160000000000000006900000000010000000005600500000530000000000000000000000000000000000000000
SP490Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Porphyromonadaceae;Porphyromonas;sp. HMT930004000000000036000000000122860000000002382000000190000000000000000000000000000000310000000000000000000000000000014000000000000000000000000000000000000000240000000000000000000046380000005960136300000000041780000000100605000000000000000000000000000000000000000000000000
SP492Bacteria;Bacteroidetes;Bacteroidetes_[C-1];Bacteroidetes_[O-1];Bacteroidetes_[F-1];Bacteroidetes_[G-5];bacterium HMT511000000000889000000000963000000000466000000000265000012000006000009200001310000714000000000700000780000103000015700001745000011800003170000268000000000300000014700001150000199000024000003060000138000024400003110000000005800000000000000250000000000000000000000000000000000000000000000000000000
SP496Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;oralis000000000278000000000104000000000441000000000750003644055542577002953000274606157203800215000500000109600059100000108200045095580144300541000443905527114006560001000000054000876000001670004680011951108006700012783083852006030005600000422000847000001660000000005100034904240009000039603218008300012250555700630000000000000000000000000000000000000000
SP499Bacteria;Firmicutes;Clostridia;Eubacteriales;Ruminococcaceae;Ruminococcaceae_[G-1];bacterium HMT075000031600008950000914000076100003900004600008600007018554313741350050880210182460126009021016680797260000114052103973400001660985552294470013059559021565517865000028752087203701500038010132953300005250127891313908000352890129052642824120010616128801089577200260006984970162063406400049411300002764000021604516598900021010204372873131674004322063814627532060002721040000000000000000000000000000000000000000
SP515Bacteria;Synergistetes;Synergistia;Synergistales;Synergistaceae;Fretibacterium;sp. HMT36000464300000014200448900000027600536000000135700266700000034460066100000022200712900000041300514000000320024300000036900435202200008100092096000010170020801900006060060260000660005880143000035100723034000050200146019000002550053201220000544004800000020300190000007008109000011000000000120000000000000000000000000000000000000000
SP52Bacteria;Firmicutes;Bacilli;Lactobacillales;Lactobacillaceae;Ligilactobacillus;salivarius00000000000000000000000000650000000001000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000002400006206000002500003200017014362340702300000006592311202856200435225252118750192542391175630811292024411394269817257811413010816642166231879112515710920141446217
SP524Bacteria;Spirochaetes;Spirochaetia;Spirochaetales;Treponemataceae;Treponema;sp. HMT23100000000022100000000014600000000012600000000016700001210000416000000000406000000000000000000017400000000019300001180000150000000000131000000000110000000000490000000000443000000000201000000000350000000000430000000000000000000000000000000000000000000000000000000000000000000000
SP528Bacteria;Actinobacteria;Actinomycetia;Actinomycetales;Actinomycetaceae;Actinomyces;sp. HMT89600000000000000000000000000000000000000020800000000012900000000000000000000000000000253000000000115700000000000000000008800000000024900000000032000000000580011700000000014000000840000000000000000000000340000000002200000000000000000000000000000000000000000000000
SP53Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;sp. HMT0640000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000100000000000000000000000000000000000000000000000000000735000000300137602100001910012602900200610071729102500001910000000000000000000000000000000000000000
SP533Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;oralis_subsp._dentisani_clade_0581500003600000000040000400091475900000001310000000000000000000500000000000000000007000000000000000000001310000000070850300000086501300000000000000006000000040640000000006900002300279614693114149122340002402489023000065037618046000084039858012900000000000000000000000000000000000000000
SP534Bacteria;Firmicutes;Negativicutes;Selenomonadales;Selenomonadaceae;Selenomonas;sp. HMT479000030002610000008900387000000900071000000000207000001130104000000023014700001390019330000000000000000002300332000000026112310000224002328000003330010100000000019141700000000031620000128000120000054000000000000016300000000190000000001520110000000000010000000000000000000000000000000000000000000
SP57Bacteria;Synergistetes;Synergistia;Synergistales;Synergistaceae;Fretibacterium;sp. HMT3590000100001423000000000753000000000000000000078000007900698103100001400451656205000076300222662000001360067105199000010008151695496000048004332033733000039003282383600000270015106890000100004511724157000034004853676307000046700180011552570000296001759678311000000000000000004939000000000010000000001300000000000000000000000000000000000000000
SP571Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;falsenii00009994000313331360000715200028187000003827000099000016990000263045092038820033850140808744240560700100475490709065665042780028800270143230405900175067870912637303450015425201004804715902023180019665530790608510160936004670826703468590030400910868305848270303858002246784397430132597803532008104341512401092414130869700560311214240875121550432400837051964300002960000333971042160220039081015293540227008336106016061202400430220000000000000000000000000000000000000000
SP577Bacteria;Spirochaetes;Spirochaetia;Spirochaetales;Treponemataceae;Treponema;succinifaciens00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000947256350001450072697719925018500054063192324742202441973026106921209630
SP578Bacteria;Proteobacteria;Gammaproteobacteria;Pasteurellales;Pasteurellaceae;Haemophilus;parahaemolyticus17000003000100013070003700060490007000235300100241130001100041151000185033312200000027110000000190150001700000300017020161900070000133300220000000004700000440028000000007000030000031102055043206360017138474182545280202303001097381200371404866110845047505361041843804693380000000000000000000000000000000000000900
SP594Bacteria;Fusobacteria;Fusobacteriia;Fusobacteriales;Leptotrichiaceae;Pseudoleptotrichia;sp. HMT219002034000020000130000017100031100000002350271800000000037538200000000573000017000460000000000950000000011430000910001000000000025300000000051116000000001060000360000000007000280000000000740000000012713000070005160000100009214000000001390000000000000000000000000000000000000000000000
SP602Bacteria;Firmicutes;Clostridia;Eubacteriales;Peptostreptococcaceae;Peptostreptococcaceae_[G-9];[Eubacterium]_brachy00094239199008237000928228540565130001389810701520000108512520210193000013016840526731531400043134597232709731386000877166170279274139850007285847571147887750006898573062226162500055538935503792435200040171003456961500009214600282952270682046934559419321801110660102333652639109012102760345172277522170900677070027439883832564400022243870293500033091951950008202041149015902908008000000000000000000000000000000000000000000
SP606Bacteria;Proteobacteria;Gammaproteobacteria;Pseudomonadales;Moraxellaceae;Acinetobacter;tandoii0000000610000033000170000000103037000008021057300020832726312245311060009029245114656837000046055802251072835700085509410533324000352190000001842282307000006508618000000004536000000025028080001300001300000001200150000000000039000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
SP61Bacteria;Bacteroidetes;Flavobacteriia;Flavobacteriales;Weeksellaceae;Weeksellaceae_[G-1];sp. HMT9000000000006500003700001920000000003560000150000301020965900000010066247000000007700000002304370000000200149300000000714904000013058000000040620000000001477050000001336015000000120000000002700000001700002820000134027125029007000403700000000018060005000000000000000000000000000000000000000000
SP621Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;cristatus_clade_578038207019150024015407704776300840447047900920043040502800024700950122014605430000600019401100593570057602804780011003220120508204325008920159044014664000103017090245800891070013901818660011600390490832990010574476040021400032070411072300020502302829017000387013803302340049904101750161082001170383010504472800598016102180112990016302800149304372649007480730000000000000000000000000000000000000000
SP64Bacteria;Fusobacteria;Fusobacteriia;Fusobacteriales;Fusobacteriaceae;Fusobacterium;simiae170329187188004039850140517318711914741110208061414623382981128578192107106753146491702030743465468436394411634143309214217387776628292591762162281820161439044041022108368154281072912547144329590780331741807361799890505875241316531657584392371400946913329183881749699837975558419923100224044171038645820271023487343161593393051242026450958206592256416492700817355083372645318789697926891401925513404373315820913669648656252019116152208858503935155677713409974694461455875211890727113169814603855435757461490649440585136035088741215463149615681520017673730108419528149814429645911871361263212026466027079837141161624749591810446937351218614951286236371768567131213198216164196190000000000000000000000000000000000000000
SP640Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Tannerellaceae;Tannerella;forsythia0214010900825007370077102679012939207787501050341600100000110239701580060165106810256084007606730147701279110030800033102275032246705300110402729013737500004821017910814001924804175024730246013537900073402537000037005860307100000008473890001402087099800212000641014302640001703161048000673562009046725580190787392005501210100022504101004450270042801860490000015901000063800000000000000000000000000000000000000000
SP65Bacteria;Actinobacteria;Actinomycetia;Actinomycetales;Actinomycetaceae;Actinomyces;johnsonii283342748012831200222171193854107171001001871791530020073513882480000075158863352586839471582083281068473779107251340107262243140030000641770484011000100153019402313992454551744672944085068301955124313147218900000354633090103500739022523943330448742681771101420512189038542495640000003255657958600705577564431018508545232027054410318157156106283111516703447712431376843604001040000000000000000000000400000000000000000
SP66Bacteria;Firmicutes;Bacilli;Bacillales;Staphylococcaceae;Staphylococcus;saprophyticus303700900003501800000003200009000056000000240000000000004000006000200000000032000000110430128000110000404000040031000000601400000000240120000000000000000000000100000006000300000035173525950510534133363980067790557802572118225132242222937978447695629400039272000000000100000000006000000000000000000500
SP661Bacteria;Tenericutes;Mollicutes;Mycoplasmatales;Mycoplasmataceae;Mycoplasma;buccale001311135233216031300008527257312826410002716855312900990000456737180016740003519437283486514450175037999964516145600710032031743730719264700004956041332442661110390271116841161450398085030621522900013480290112235594600103941108451276178007909016612882214300178918429094346011100117211014302318355180088510860025413980252001597110299022232300050210200800420007922398000000361840000000000000000000000000000000000000000000000
SP67Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Anaerobutyricum;hallii000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000033000000000000100651006635106223682211518150429214145300114345981254721190011558652407419612782306234820280
SP672Bacteria;Fusobacteria;Fusobacteriia;Fusobacteriales;Leptotrichiaceae;Leptotrichia;sp. HMT21202000233470010000003811090048500000654200700000212100000040066000000163370000000015190023000705000000000056000000000330000006013880000023013500000102000112000025540317800000020539003005500150001585031201663482621431122728983941021542710011601118063834852314702280000000000000000000000000000000000000000
SP68Bacteria;Firmicutes;Bacilli;Lactobacillales;Lactobacillaceae;Weissella;hellenica0000000000000000000000000040000000000000000000000000000000000000000000000000000000001600000000000000000000000000000000000000000000000000000000000000000000000000000000000007000360000279634281612461436642556300000000000000000000000000000000000000000100600000
SP682Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Alloprevotella;sp. HMT473051338058014300000000032000163279507851000531296407066000000071750004300470400000086061110000040175085290000000000138250001800006520000440002786800000000931310000120000887252000210000375104000000036787720000000029714336400018264180711179586233511284802987124608288251911711540186143963812349067212831677804514285197061310290510159002890000000000000000000000000000000000000000
SP691Bacteria;Firmicutes;Tissierellia;Tissierellales;Peptoniphilaceae;Peptoniphilaceae_[G-1];bacterium HMT1130000390018700000035002270000000001400000060023500000000011300000000090000001680030000000000000000000000000000000000000110013220000000008000000000000000000000000000005750000000001500000020000000000000000000000340000000000000000000000000000000000000000000000000000
SP705Bacteria;Bacteroidetes;Flavobacteriia;Flavobacteriales;Flavobacteriaceae;Capnocytophaga;gingivalis008500000134000640000025670014000008710000000002500031200000750000108000003080006700000364000000000303400041000008300080000003400023900000188260017700000409000230000003600000000046004800000824169003200000443240081000002820001400000036600049000001720002900000188240000000000000000000000000000000000000000
SP71Bacteria;Actinobacteria;Actinomycetia;Micrococcales;Micrococcaceae;Kocuria;rhizophila000000000000000000019000000000000020000000000000007000000000100000000000000000000000000001000000000700000000000000000203504000000130004000004000026000000000000000000817600001565157263708000430231614436041188210183091128213180021015602925000000000000000000040000000060000000011100
SP719Bacteria;Deinococcus-Thermus;Deinococci;Deinococcales;Deinococcaceae;Deinococcus;wulumuqiensis1915103612121270903262008252702802225814003805501355402901002150100010800000009939823800000000570000000000220000000008004438502500000421300000000000042000019000500800006000000000500500110000000000000000990002068800001783120446003600667746154000423477825474499421150520099000000000000000000000000400000000000000000
SP744Bacteria;Bacteroidetes;Flavobacteriia;Flavobacteriales;Flavobacteriaceae;Capnocytophaga;sp. HMT878660001324504118535802153016424290099259532682959782017915600075734818816335393053127830011928361719103311930136400002301875042091285012780016315056267117106204500406789039209900081300254172645935620000027889139536551971060900531102988287363706950055641891846125902098003832723317152798098002871533823812717840320027210449182125121901624001716357791225513240117900187533431073771264011100342140434681080108230042346509700720055201021812520670031276729064300000000000000000000000000000000000000000
SP76Bacteria;Proteobacteria;Gammaproteobacteria;Pasteurellales;Pasteurellaceae;Aggregatibacter;sp. HMT51300170000000000000008100094000011610005100001150000120000000000000000000960000382000319000011480000000000000000000000040200000980002740000026100038000000000000000000016300000109000373000001590002210000047100290000026300020300000426001400000019400000000000000000000000000000000000000000
SP760Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Lachnospiraceae_[G-8];bacterium HMT5000000000005000000000090000000000190000000000000000000160000000000229000000000000000000019600000000041100000000058800000000034200000000023200000000027600000000021100000000075000000000228000000000270000000008000000000000000000000000000000000000000000000000000000000000
SP77Bacteria;Bacteroidetes;Flavobacteriia;Flavobacteriales;Weeksellaceae;Riemerella;sp. HMT32228701992218900928408402471719112020310902850180722218049163070280190160020517721058245013023022022334541072600291003760585624740016700850276453358000007100430242187000009340013331180911617000614641906000159000300158636015000483038419955704036093005474730300311900001450680040110004160000000450015700001001720569812301600312070331118004900244090441259001862003776048720080084500000000000000000000000000000000000000000
SP79Bacteria;Firmicutes;Bacilli;Lactobacillales;Aerococcaceae;Aerococcus;urinaeequi2502700000000046000000600000913001061200360000000003101600012000150012000000805700031000000000132100053700000000000230000000286070000000000000638411070751301604313380020325000247680008028000111300011107471203293591904122786521070008620226819311297600121262322123478686419111911810127130000000000000001401600000092103936000077581305001000
SP793Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Tannerellaceae;Tannerella;sp. HMT9160000000000000000000130000000000000000000000000000015000000000480000000000000000000000003660000153000074500004370000000004400001100008800005180000765000044800005090000955000023000025800002800000000000000000000000000000000000000000000000000000000000000000000000000000000
SP796Bacteria;Actinobacteria;Actinomycetia;Propionibacteriales;Propionibacteriaceae;Propionibacteriaceae_[G-2];bacterium HMT1920000000000000020000000000000000000000000000020200000000012700000000068000000000190000000000190000000000800000000011000000000213000000000140000000004000000000000000000014600000000060000000008230000000004930000000001368000000000000000000000000000000000000000000000
SP8Bacteria;Proteobacteria;Gammaproteobacteria;Pseudomonadales;Moraxellaceae;Moraxella;osloensis1202500050000014000000000000273003740000017170102516200133200266199235001700000400033500224226615070000051393008221000002200000540831000019430000000004238500452900011241803093100401900017110000146110004000800781020036495319958413879100249114939313177186104440787131030175112199217469112012877213203600005000000000000000004081300110019010000000
SP806Bacteria;Spirochaetes;Spirochaetia;Spirochaetales;Treponemataceae;Treponema;amylovorum00000000000000000000000000000000000000000000738000000000350000000000893000000000254000000000780000000002300000000008200000000011600000000013000000000020000000000012100000000010500000000000000000003600000000000000000000000000000000000000000000000000000000000000000
SP810Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Bacteroidaceae;Bacteroides;heparinolyticus0000000003400000000034000000000000000000000000000002230000000003220000000005000000000115300000000017620000000001687000000000358500000000099000000000086300000000041100000000000000000007730000000004700000000023000000000900000000000000000000000000000000000000000000000000
SP86Bacteria;Actinobacteria;Actinomycetia;Micrococcales;Micrococcaceae;Micrococcus;luteus1000000000005000000000000058000700000000001700000000000000000000000019000000000000096300000000000000006005000000000000000000050000000035000000000000000000165700000000004516438040622915179113622976045825055271311626927207126662874813712578215210320140691800000000000000000000000000000000000000000
SP90Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Lachnospiraceae_[G-3];bacterium HMT10008450050015300042800820016300068200110018000118002800000012000040000005240080000008040000000003700000000003258000000000359000000000075200500000011320000000001033000000000503000000000468000000000123000000000740012490000007001140000001240050000000400299000000000000000000000000000000000000000000000
SP93Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Alloprevotella;sp. HMT912000267207440732301460000247173323618302410000227925300000055631611039000003080000000302403030000080006000023709010000001075029500000131707900000260000000005200000000321299281549039900001779024544765471000081134648371050000111591196480480000304184109130190000001000700000018001600001463451602100000000000000000000000000000000000000000
SP95Bacteria;Fusobacteria;Fusobacteriia;Fusobacteriales;Fusobacteriaceae;Fusobacterium;sp. HMT2482318530405915758149200962001950741234113523553523211738293127171101029127981002875833327213929367237072183415210138458265024850190329154781482002582261319886547552714479151212938462461524141001894551802348641320400219595664811948217050161571811111219922977309558529219131651316036101101864190731510088139711915606193141513510114882144063189105715802089583821521341788747961812503776127791055094424186425009665552180416511332501220192083125262173856383526411358239726579452514281167472452791609607841052563502813580000000000000000000000000000000000000000
SP96Bacteria;Proteobacteria;Epsilonproteobacteria;Campylobacterales;Campylobacteraceae;Campylobacter;gracilis000000000190000000009700000000012300000000012120000000001580000000008673000000002817400000008700000000014800000000062200000000870000000009505000000091023000000020540000000021000000000020100000000016000000000001000000000012000000000000000000000000000000000000000000000000
SP97Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;lutetiensis13900400000000000500000000236000000070120030000000000800013000005000000000000000000150000000000000002000000000500000000004000000000000000000000000000000000000000000910921049115810111600700102393416042611201725137422520340271104180400254138545726718084394155625258017893947751911168984810443430594324119872979633025783128937125692507853387622302386351348462694522061313466616045484764631311345
SP99Bacteria;Proteobacteria;Gammaproteobacteria;Pseudomonadales;Moraxellaceae;Acinetobacter;lwoffii00171000000000278000000000000000000000000140001800000014000000000000000000000000000000041600001210000000000013800003200023300041000012000000158374270082000184444910044200010348810000248000067000800005000001707109001120800001200017371180002020000040000000000000000000012000003200000000000000
SPN1005Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;cristatus_clade_578_nov_95.344%2800241446118820000099230212108060112651404130387090013053964430290104717075025502723390080466001843000324576243584332435470010811414313187471936600001326294602202510986032199032154316215449119318104760182761140218686645114724266017719104250468115311011452492980269646027816177222449658913352631148776123904257229613439587842713025918741720372433062169376512142567191919722222492260430205184199725139922173805267997367882432042296774124239100000000000000000000000000000000000000000
SPN1016Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Alloprevotella;sp. HMT473 nov_97.331%0000185100000000012210000000007180000000001360000000000320400203100000056560013097000000567002243000000690002705000000840009730000000255600495400000044400011700000001315003200000028730031120000002255007340000004740013990000002704001190000000490000000008600379000000280006000007600265000000000000000000000000000000000000000000
SPN1038Bacteria;Proteobacteria;Gammaproteobacteria;Pasteurellales;Pasteurellaceae;Aggregatibacter;sp. HMT513 nov_97.331%509335300194002617711322464124005044105584312430713001017001708163788046550002418003651680789114112003370052298715442370037100741411201364220016200294663160528720067000133343601900025780001101145461900130096585551807000100000456159015609001935009629740568940021004619343317870033002291271010250006300259801961080001800052762007550092001637140759172102640098106176058001480065442613032001490026260460000000000000000000000000000000000000000
SPN1049Bacteria;Fusobacteria;Fusobacteriia;Fusobacteriales;Leptotrichiaceae;Pseudostreptobacillus;hongkongensis_nov_93.008%154500123759211560020600011292010004360005218225448350225001125194175290375101711149147820043318020090256102005613720241823875900851622702417751415100040011321030001800003000001370088313823900380001664404100118702062232015024580943414100019374142111262217330611139529684027158045113300947680354427218674490438540708552974721747259329032119423164415912819861345238203791162801974841173715971212549891959440000000000000000000000000000000000000000
SPN1053Bacteria;Firmicutes;Negativicutes;Selenomonadales;Selenomonadaceae;Selenomonas;flueggei_nov_96.421%000003432294000103000027953040002570000978000025600003400001600009149700000000540364000706000020514650007000018919970000000046000019000064100001060000502500275000010200000000051900000000020814600020400002012524700183000011194200010000010000000000382400012000013700000000070560000000000000000000000000000000000000000000
SPN1061Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Bacteroidales_[F-2];Bacteroidales_[G-2];bacterium HMT274 nov_97.723%097023256942148336732063163404210176312961216345550622632239017660147431540514922429590960280034265810190336003950085360194753697612006638165870566801547825697148267340659505397022046521234018923639333166029180985201205133005501409507032760029933776558032842441164507402043933331132301396208859809190201376100722418124600156802725463860555236124001399017362039286253398327088480937040312356731025903271118617583061015869258364702259414593209660119044263406536236199806006563015132036260037010671281955561208170701192080173218034804215330069100000000000000000000000000000000000000000
SPN1062Bacteria;Bacteroidetes;Flavobacteriia;Flavobacteriales;Flavobacteriaceae;Capnocytophaga;gingivalis_nov_97.689%36002528217005170278228001377248003070188108500112186001669031993200854080059402435470978115810944597158112390403849635441128081164316222005631035021363125631583677028785162178844512890370117619473210190210925135240982151226034410251013132970682731110288201751658126046137617002192103124952018311711427966613494337730166712309013118251459601061011044487923711041311249882294332662336247165674671131001967100069013855001504310232541000274614887199110701148969681146736019293274590000000000000000000000000000000000000000
SPN1073Bacteria;Proteobacteria;Betaproteobacteria;Rhodocyclales;Rhodocyclaceae;Propionivibrio;dicarboxylicus_nov_93.802%0167117445358004758341103976911194603103005478064451326223679547006750751532241010445300477089941221382104369600106032987136486295542220081363010215772992423822180062900662151619595329001990730139446489326006641900131419117881810572800135892713010192171915641870010060158159043716841221005040156024084690148001412133517010421296834150021207811564943574978757700160902606591564705549200130509100154912300008201704150021006151134142800000033289870091500000000000000000000000000000000000000000
SPN1076Bacteria;Proteobacteria;Gammaproteobacteria;Pseudomonadales;Moraxellaceae;Moraxella;oblonga_nov_94.387%74876371751294656565779422415717271368556252151479224067119206229610262088186867823108987002522521823972160518447110488173544893817347128910333484243276103449715237210720189894610457104149168823951923861975101024413421117818628764117621908717139152765665185813270259590310883670363719330258350108973952596396050527194796934513173179162353391082929611016618116453681440141531942246246941056474621258582213391512925482247711960715731881285121371391179112513732470585291124185988192954652256421262762329114793134580392542124650664386412783812362168393534120811437861815949591174557001079325192145437622271041532213626225170769516183986674787121210000000000000000000000000000000000000000
SPN1087Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Lachnospiraceae_[G-3];bacterium HMT100 nov_95.607%00013812000000000565100000000063500000000031320000000004181851737510000008922936694070000003050118920000006166023890000001586817670001500058042090400412000013060000000300000000020008264722900001406501709641000009001302079154800000134611816180279000214000000000195225130000008411000000000180010389510000000000000000000000000000000000000000
SPN1098Bacteria;Firmicutes;Negativicutes;Veillonellales;Veillonellaceae;Veillonella;parvula_nov_95.874%30300022106313177003001000182819211800146240004344782192338420168131052003186860211287106200002612571900028000185022213000293004516111566364029010276047855331001290000000193607012350000308000004467540130246110772004698281060057134425912402000008692000400000260142000746126063155553770014230185040919941213930480015702149578905368810031515400281610058122017330792600107515020000000000000000000000000000000000000000
SPN11Bacteria;Proteobacteria;Betaproteobacteria;Neisseriales;Neisseriaceae;Neisseria;cinerea_nov_97.541%28140000000000100080000051000180000003100000000000015000000700150000001200001313000000000000000000699000000003200000000181700000001028173300000000000050000000000000004000000000120000175125633501322601059129244330196013218777271302553901636218307135193166911840011456928503154523830312000000000000000000000000000000000000000000
SPN1109Bacteria;Bacteroidetes;Flavobacteriia;Flavobacteriales;Flavobacteriaceae;Capnocytophaga;cynodegmi_nov_90.208%7950000129298122510119800007871350717077000064280371056980587000074011178127705800003329562723151504800011290219888630000424022382471180100000102122468172000007201411320501000032146656013200020326525522083200000620291572662300125200069180485479167553900012014938379675488000101645115098257103940004119264313756193800006817748433000006189332491880990008272683526286000093328124120000000000000000000000000000000000000000
SPN111Bacteria;Firmicutes;Negativicutes;Selenomonadales;Selenomonadaceae;Selenomonas;sp. HMT919 nov_97.244%000000000269000000000207000000000280000000006200000000022400000000022500000000000070000004720023000000427000000000120800368000000115400340000009650080000000175000000000768005400000033200173000000715000000000149000000000000000000014001100000000000000000000000000000000000000000000000
SPN1121Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Porphyromonadaceae;Porphyromonas;sp. HMT278 nov_96.107%546000000061905640000000617034000000042650000000001327012200000001700000000016620820000000534000000000010450000000001551054940000000117702670000000239902257000000088103651000000012801823700000001463010940000000141001150000000102309000000072080000000640230000000470340000000000000000000000000000000000000000000000000
SPN1133Bacteria;Actinobacteria;Actinomycetia;Propionibacteriales;Propionibacteriaceae;Propionibacteriaceae_[G-1];bacterium HMT915 nov_95.879%0032600000023330029800000023130000000003915000000000102817196843600000010158626059000000149000000000110001248000000087701487169200000035105204137000000122000800000017800638000000024401882851000000553333816118700000016260441116000000296201860000000706000000000591033000000160112100000048008000000320000000000000000000000000000000000000000
SPN1145Bacteria;Proteobacteria;Betaproteobacteria;Neisseriales;Neisseriaceae;Neisseria;flava_nov_97.947%0182000012307150530002121001086800000250191901931220700021123490052430982743019866404871306807017976901322500804140562702835320070082428303490000013517380800380663501310400901029694812630600550018110322429732738918008901671019249016186430311931000050117517315018191900844141172119146020490140091629350133520801530692155650237108215001381903051109916227131390145062882418563971604033201200000000000000000000000000000000000000000
SPN1157Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;australis_nov_95.152%002000000340000000009002300000000000000000915000000390031192120000016301032500000017280178011635000003483088000000000639000000002160000000018002000014005088578197001810073535740014132400402712080018642060033151688399900104765000004300000691255751253290000585022136827911300005741021063903622071002040188350561750000000000000000000000000000000000000000
SPN1169Bacteria;Firmicutes;Negativicutes;Veillonellales;Veillonellaceae;Veillonella;parvula_nov_96.660%913006243719011930412421602901135002470517075760545243825811360367606102083292020748002790410572109140147001226045317450016209992755078613900402082170138026933641253608000252163450003930795700966126395105069552150720145111024023120012113218542386566056000372623990840120004175031174022608343154041513500239012023882021992051924920161722707031429012225010702645239200164071850242133696149880311308821230620732850011702760000000000000000000000000000000000000000
SPN1173Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Lachnospiraceae_[G-3];bacterium HMT100 nov_94.990%00005450000310000930000013900003110000000003450000000001760013403640000186001910590000021800002800002540000468000000039801185000010020015307990000299000010420000470000126400002610000155900003870032001273000045400466067800009900428070600009500000000000000000001900000000029000000000000000000000000000000000000000000000
SPN118Bacteria;Bacteroidetes;Flavobacteriia;Flavobacteriales;Flavobacteriaceae;Capnocytophaga;sp. HMT412 nov_95.597%1257005230126100211103802006004854888001091015161255100374452550069560596758000408041010013680208531004186783678438597190131440000934930497821653630883748002209609280083031027008830086925195896847670000002139569319334403633950198517001621138935383664972106107673001782210320065134129550458100201239829151544196458340231000570628915411429325131310419980049438413551179122311143048312001762241969254911007762716990020621291754477778431826213061282839400254050690840105203160137007700382871580391760167001761605762911122600000000000000000000000000000000000000000
SPN1181Bacteria;Proteobacteria;Gammaproteobacteria;Pasteurellales;Pasteurellaceae;Haemophilus;parainfluenzae_nov_94.082%1568589279264879822502020387201041192185216731655124725493947273266392246667244745109922742372721103220590192643192661691112123510253386022930234133112886270288274918663165192991816495231670394535332436158922503550253190399841948111951110090401575451604917110113510260182301319469245368919063381435139937333628328413970148203233701486361627340884252052093286447498446313456637931655264234978184061191861841182548204564117294714920056844919184317569989631093516782165413452806713341459289702479695619469025554656986402240201273534168246752721216960389148816214125965875451216149412800000000040000000000000000000000000000000
SPN1182Bacteria;Proteobacteria;Epsilonproteobacteria;Campylobacterales;Campylobacteraceae;Campylobacter;rectus_nov_96.957%3313089829048811435371460185891992150295761240622264681343158235323128753019958995312148028138390936156471103864759522239169503771370793016546135314458129979477927642492539947376121229292333913637828250355501920511040517444102841221371349152932745300795785615386503035200003145540356244298591420334218375492013341797163022041913010022717957939994515304390310119021117739125871467136085105162657327265484639891018196844876134500135528910425919312727152180034222652318003252448224536313637350043590000000000000000000000000000000000000000
SPN1194Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Lachnospiraceae_[G-2];bacterium HMT088 nov_94.958%002668000000100014930000000004400000000003030000000001778890000318700018513820000484800887109600001479251008734200000104547003565210000317200041110100001501170293264118500001472002415101620000151217046941735000001202392139819000280090704291800001698273023149840500006821500935000000940011851200000911130744761120000418620100206257230004281300000000000000000000000000000000000000000
SPN1207Bacteria;Firmicutes;Clostridia;Eubacteriales;Oscillospiraceae;Ruminococcus;champanellensis_nov_93.952%000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000100000002000030030481480000000150000584324456685944551231257817334491486386454531170378955151826579269821694323472420228814782778283357548821532106434925137735021202460330
SPN121Bacteria;Actinobacteria;Actinomycetia;Actinomycetales;Actinomycetaceae;Actinomyces;weissii_nov_94.130%00162091032010500876001560297511305500147007043810252960140100003083002403480019051011301034302001001230747411873200100700860827214855160201380016001060864280796053600358701687349238655141068900001487419037705801003250336333561293785030030089021461363573540122700336020463753185191362631100004338306056922414391009802735608705127502161001100086710056045230187100208012780000430055202818000400142601142788004300113908917000140000000000000000000000000000000000000000
SPN1219Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Porphyromonadaceae;Porphyromonas;sp. HMT930 nov_95.473%32830003800450044000071002200460000590022800678000125002190055000361217000330001160075000000580000001200059113000000009950000000013992900000041816060000009330816000000112000000000403000000000138200000000043195300095840002359009400368261002161667794301183003640016047991488513880025900219232938905961400000000000000000000000000000000000000000
SPN1230Bacteria;Bacteroidetes;Flavobacteriia;Flavobacteriales;Flavobacteriaceae;Capnocytophaga;sputigena_nov_95.754%0000000099019260000000075912140000000074632000000000175215303303566000014286000878000012730002726000000948640001917000077213200028730000281111000189700002521040022157100009116002520510000743040001622000017102100039740000181045000212800000181200026080000012860000000076389001786231000017731001169608000051400037533000001450000000000000000000000000000000000000000
SPN1242Bacteria;Bacteroidetes;Flavobacteriia;Flavobacteriales;Flavobacteriaceae;Capnocytophaga;sp. HMT332 nov_95.763%02147138300000038605691143000000354016381670000005350198511000000029202072163000000911047421230000001700104385900000095806403670000007880889111500000014402599170000002190997153400000039201127123200000028905051188000000298063161024000000275032797400000015900329395000000474016463500000059094190500000015022337100160006604553000000001420000000000000000000000000000000000000000
SPN1253Bacteria;Firmicutes;Clostridia;Eubacteriales;Oscillospiraceae;Mageeibacillus;indolicus_nov_87.238%0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000040041800000000000007440000592400002555000016440000462700000737400012049000092390
SPN1265Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Lachnospiraceae_[G-14];bacterium_MOT-185_nov_92.521%0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000006000040000027130000000000400134610162811532261439413035378347491157227219692300013601942332611086182119882509113353393219379908771586925475878396313672
SPN1276Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;sp. HMT317 nov_97.746%03800385001317127213650180025700978778170501000414001080771119014300267001454184227600001560066823142602600340022857717620000490007258541400110036000631664143300001360067079772600009004072425050000281001295799846000061600173157220200002000389134164018000002355116750000300050648415990000215001070518330340000011726010250090054382302360003000263701720014003724410000000000000000000000000000000000000000
SPN1288Bacteria;Firmicutes;Clostridia;Eubacteriales;Peptostreptococcaceae;Filifactor;villosus_nov_97.484%00008160000181000001034000019050000207000034400003000000285000051300332048800005100010580894000010680000140000321000084700005580028210137500003888008030777000094600358013170000264000018110000190000187016450000352400142016790000215300633020500001194001200632000058500003920000410000000003100000000019000000000000000000000000000000000000000000000
SPN129Bacteria;Actinobacteria;Actinomycetia;Corynebacteriales;Corynebacteriaceae;Corynebacterium;durum_nov_93.865%1400023080000000572475000010250720235502559800437058033614640000207000164015309392100016300164200053010626820005402300747700000000000059090000012206500040100444333200000122003497515000980000017033028000004316125000330004139601904703130054980005701740426066401004901130105896334190702450115591280220510464017137065900000000000000000000000000000000000000000
SPN1292Bacteria;Fusobacteria;Fusobacteriia;Fusobacteriales;Leptotrichiaceae;Leptotrichia;sp. HMT909 nov_96.739%142101314963902141312418144681703101824371952617694945266193540531620534104932020651237658177021095599145904400201111503490532179667034023970468806806345568380322249735537646052922306360107957953913400214747892854078912250177180883462050121147994579504293391145096014330242022068175377734746330381500185119671821138103569062880197732322952930520310290076619758002571270111060431640341531547001263106507479204041125118603711760363323316885288622713571350988224116543011967228217286377831616313745623900371994225143742790530820000000000000000000000000000000000000000
SPN13Bacteria;Firmicutes;Clostridia;Eubacteriales;Desulfallaceae;Desulfallas;thermosapovorans_nov_82.255%0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000110000000000001702595205739111165273583393599707310666802764903773962753991451114010511184624700334221644616
SPN1301Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Porphyromonadaceae;Porphyromonas;sp. HMT275 nov_97.125%0324400000029160027460000003488004595000000796004300000002916001250467846930979019320570300122814192603063261351029810052826331312202214519503161002230754322410326424003733001369111421430993400610700379132261994086890025950021634568415302290670030614338246001359005060013643063398206946259109345009629130499704938546025820014765619709004198142502584001879146843890273528330472700207000000417001978463314014076011380070320005910199101628900575109561806315370113300000000000000000000000000000000000000000
SPN1302Bacteria;Actinobacteria;Actinomycetia;Actinomycetales;Actinomycetaceae;Actinomyces;oris_nov_97.006%22400218073105001070026450241090112700088000904200013123700024910587818080401870171101094807880020900000827002006288170005400148013470043541025180760003753200470510038400050103000020001020306200037118004600325001831200960620005300023035138000204004603318000304500470413700216276300940190022332823023970100710020513328007904740000000000000000000000000000000000000000
SPN1304Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Alloprevotella;sp. HMT473 nov_96.311%00000000000000000000000000000000000000000140000000001090000000001100000000000000000004000000000051600000000068000000000430000000006027750000000012232180000000033000000000004756000000000000000000050000000001700000000010763400000000000000000000000000000000000000000000000
SPN1314Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;salivarius_nov_94.949%584800126109005120020140303300002273882700890015491132800005140130010290008020120560260030600937000061015902000039034034210000048006380000288300000312126013052009000000030700802020004000000000006011800069216303239121914864621353437317350470512178517371088117714752724988439392739244256049539623344133656366481141802162251984011544122680000007000000000000000000000000000000000
SPN1327Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Johnsonella;sp. HMT166 nov_94.154%00000000016170000000001923000000000993000000000827000077400006360000635000070100002867000041000017530000130200004440000210900003223000082600001734000013450000627000036180000106100001150000148700006240000232400001464000014350000100400000000049800004600000000020000024000023000000000000000000000000000000000000000000000
SPN1339Bacteria;Bacteroidetes;Flavobacteriia;Flavobacteriales;Flavobacteriaceae;Capnocytophaga;sputigena_nov_95.966%03870000002868132099200000022828206670000002195550850000005168904750010004461104690000003565001147000000285323091100000023466067600530007901708250031000800009740039000350076500300024990930400000807301390000004414704721400000011220508002000031505600000018825091001500047100224560210007290204140180000340000000000000000000000000000000000000000
SPN1350Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Alloprevotella;sp. HMT473 nov_97.951%000050000000002000010008600000000038000000804004000060202700001702841026200000008797200000000660000000280000005602766880000070947390000000003400000260400800000037012110000012731405314000090019600000013030138641448200000954017371414550000001968805822533820000014110000000000000000000000000000000000000000
SPN1362Bacteria;Bacteroidetes;Flavobacteriia;Flavobacteriales;Weeksellaceae;Weeksellaceae_[G-1];sp. HMT931 nov_94.561%89222300107040561900457000039402112004922601410294860615703209132800059773302227100480241824019271922035521168870030488933399432311026001530008820400077022001372005942035160227160002860020184600015401200060001767140119064001723512451228040142415549712912240011198967519134383131134264135691557122989122200818523932011917761183357544412341567107133425115954814596803728196342071782400000000000000000000000000000000000000000
SPN1374Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Porphyromonadaceae;Porphyromonas;endodontalis_nov_96.327%00001291000000000595000000000121600000000056900000000012040000000001020000000000848500000000011220000000002670000000003369000000000953000000000770000000000203100000000026320000000082147000000005604241300000000012700000000015900000000084000000002058000000000000000000000000000000000000000000000
SPN1385Bacteria;Proteobacteria;Gammaproteobacteria;Pasteurellales;Pasteurellaceae;Haemophilus;paraphrohaemolyticus_nov_96.122%742250202153200042803065330067300041211919313014200000278310903990008107006074140140410031809502007060830011219700015106060791420006057005150000467044230000212816040000098537136120080410005745001202500018801148130040091495962009256159911186241730121624017545056403032069364668675743451990615180928683391357544711576562171798208650266100324044151842420000000000000000000000000000000000000000
SPN1397Bacteria;Firmicutes;Clostridia;Eubacteriales;Peptococcaceae;Peptococcus;sp. HMT167 nov_97.250%000016960000000002050000000000172200000000060200000540009300082500140001034007550067000010810030080000123600000000051300262900129000165400606002180001285001010021700072000110068000946005630046200017840055500389100070500800351000720008600000056000000590009900000490001000028300199000524000000000000000000000000000000000000000000000
SPN141Bacteria;Firmicutes;Clostridia;Eubacteriales;Eubacteriales Family XIII. Incertae Sedis;Ihubacter;massiliensis_nov_92.917%0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000030000600000000000000000000000000000000029023414399109133159513206161556411068220652723021291821053934928818213923330335843311442284181154178236217362127
SPN1410Bacteria;Firmicutes;Clostridia;Eubacteriales;Eubacteriaceae;Eubacterium;ruminantium_nov_91.260%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000007000000000111711000000000000120000032459009188400001759342363601404201400264500624323960486152100234201546263200895
SPN1422Bacteria;Proteobacteria;Betaproteobacteria;Neisseriales;Neisseriaceae;Neisseria;cinerea_nov_97.951%295833653388642428737100189882532261011134028883943161160725357105918612376014611031196216167629322610491191881591603904556512445571124485296583571310319621857203105866141037242022104592105014500419409301016900143918929088689838503537800160615056444029581908492125411723280247025438221417230908821301187507030829115808502268141123417306019811721334210717625203117416867126232731807415119808925135584971316570475267130923586141684448794014152259868725106383267443315225387713761986497944434709646763293898649486228284051320304039875632737941580000000000000000000000000000000000000000
SPN1423Bacteria;Proteobacteria;Betaproteobacteria;Neisseriales;Neisseriaceae;Eikenella;sp. HMT011 nov_96.728%224167332521751532108117147858477541282701929122526316277474135743175118711099384491310251190146805920434314602525973123333123138854767812105381251928444148868572378480101222148215515513845941421492018712083142370185145008803931172297055746631624165617342997243315552481763519142904333319282565612826910102644635350497611356500276810270015491742734205154405375945529897167162468248013326237130111662271077190111002594024576193200501004183175353001711190000000000000000000000000000000000000000
SPN1425Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Alloprevotella;sp. HMT473 nov_96.715%00000000049000000000060000000000160000000001300174900000391001359000005500046912000001100567100000048400315750000001910024516100000167001288800000679007025000006830077738100000208800309800000894005361310000042600527160000024020000000001110066700000120022547000001200457400000490000000000000000000000000000000000000000
SPN1435Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;copri_nov_97.959%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000002400000000000600000600289001200000000147410167219856778971872168742728761238576298948376174213906102730366155880514981741088145095067532219934615290938612211051356
SPN1448Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Coprococcus;eutactus_nov_91.221%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000400000000000000000000004300000000000031644159443118318391203481609038504064099015680005026371179291733401030503210861717838771609154616
SPN1460Bacteria;Firmicutes;Clostridia;Eubacteriales;Peptococcaceae;Peptococcus;sp. HMT167 nov_95.695%000000001622570000000007333610000000054850000000038133000000000363250000000020232720000000045824300000000124738500000000130662200000000842420000000019194110000000018710570000000087115100000000194369600000000260017980000000014095490000000077451000000007445000000003634000000004400000000000000000000000000000000000000000
SPN1483Bacteria;Firmicutes;Negativicutes;Selenomonadales;Selenomonadaceae;Selenomonas;sp. HMT126 nov_97.239%0178157014002580850176510930048901160486340950098096079149017002290120282101580108000344040810336017400092714676471910220000006522369123000024704011795497310010090608501035710130002895379842116421000008044191068114803000073405311405562114900023101142310111170090751143124830531510001305526112511864488001904530429018500008706751680000003083843400002202705170000000000000000000000000000000000000000000000
SPN1494Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Catonella;sp. HMT164 nov_97.490%0045406180000660039501530000000990543000000025501940000266170901310081508920015078006060925260237097800388016003490143003550116500630117002284551187001200006054593825022017600918427195630008300931312774003050280054546816700680000195124810233401800000459118722000227000021881027104000210170000232240001500136015160000000027000000015800000000000000000000000000000000000000000
SPN1506Bacteria;Firmicutes;Clostridia;Eubacteriales;Ruminococcaceae;Ruminococcaceae_[G-1];bacterium HMT075 nov_92.308%221400015013003100001500002450001001800168000108019007000008001248000009120301600001118240338210004051124000000007000000000000045000019400000000977011050001900200000000001900000018702871500402439084790010226920048598108124215531213019030031736823038130159817718457516113862607141730135166198614855155344224840403530000000000000000000000000000000000000000
SPN1519Bacteria;Firmicutes;Clostridia;Eubacteriales;Clostridiaceae;Butyricicoccus;pullicaecorum_nov_87.810%2002000000000000000000000050020000004000000000000000000000000000000005000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000020000611000000576000120000089799717400002005007221223607645434206419855031933238618158068743021259678552200514042944348676035989317496172832327287935320861091273530891597
SPN1531Bacteria;Fusobacteria;Fusobacteriia;Fusobacteriales;Leptotrichiaceae;Pseudostreptobacillus;hongkongensis_nov_92.797%0068000000600110000001900845000000590019000000075001300000023300000000021400245000000244003620000006800008000070030000150319000000000810080000701340038912604162290360001352115322908002633130124004101380026420743650064021002267000000226100450000004217004101201001220290600643189712123560552017690000000000000000000000000000000000000000
SPN1542Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Porphyromonadaceae;Porphyromonas;sp. HMT930 nov_97.521%083361630179823013800410790102800909018371752380808434018590028412772240551421059210356131665018053652527646971160381064127455341205961248758606704204892185811222857609007447634952295202670060013825381670023211145140114330501111820021183151081818124406040313515002932981331769372251064635201140309481263232721401401994503897613642538597077221609966531942548359302469398077127744761937153088942642110640925710602028381679207219879710371132579768391035245283425640147711416946836652706211368151014032709436027739450664048961049011360000000000000000000000000000000000000000
SPN1543Bacteria;Firmicutes;Negativicutes;Veillonellales;Veillonellaceae;Veillonella;sp. HMT780 nov_94.521%12000000000000000000000000000000000000000000000000025000000000000000000000000000000000000000000000000180000000000000000000000000000000000000000000000000000000000001063144171347015691200117857234156010472310002593377620631600163335002657400633274001016720272027630000000000000000000000000000000000000000
SPN1545Bacteria;Bacteroidetes;Flavobacteriia;Flavobacteriales;Weeksellaceae;Riemerella;sp. HMT322 nov_97.119%00000000047000000000330000000000000000000210000228900005310000139600003020000610000371800003020000538000017570000118000015200003260000710000033200007260000172001930450000420030017600008700689095000060800629010700009700000000010300001980000370000139000010800860720000960000000000000000000000000000000000000000
SPN1555Bacteria;Gracilibacteria_(GN02);Gracilibacteria_(GN02)_[C-1];Gracilibacteria_(GN02)_[O-1];Gracilibacteria_(GN02)_[F-1];Gracilibacteria_(GN02)_[G-1];bacterium HMT872 nov_93.450%88119090001704341500280000069176807008300243819000045000000805020101128000078516893100140072821622220036001245723100000015110076100000200577183700402280070500065000004631160296004614040164511420691471718102919930324107551301697180435013691508100914232600620018414396707097534583582410827323222722135320924013902710281072007484284873906923753741990000000000000000000000000000000000000000
SPN1568Bacteria;Bacteroidetes;Bacteroidetes_[C-1];Bacteroidetes_[O-1];Bacteroidetes_[F-1];Bacteroidetes_[G-3];bacterium HMT899 nov_87.860%000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000400000000001268000000000000330201643105771370118040120292465851162486852599317710573007158334113244677177188510764019732173921019322181711272
SPN1580Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Lachnoanaerobaculum;umeaense_nov_97.689%000144111100090000053016700031300005600001600001463900063000074301736175006100068502175115022300009290012010800005770051082700034150796000350002077010200005500057000000000091400000000063901048295004900018301209135840710000010250007200054203913100000001119000500001300110191400007406107000002800189100350000000000000000000000000000000000000000
SPN1592Bacteria;Proteobacteria;Betaproteobacteria;Rhodocyclales;Rhodocyclaceae;Propionivibrio;dicarboxylicus_nov_93.802%13186170280007935132739495011700046791582131450710003353426242490073000284378329296706080003031120230121006200038613359316106722800050112726010106100025088011191197057900015218614615513970340002101571221136218570007261311723039206100014213801655920250006512080406080000216414359841814561000101416601321123051000218440015401060004041062010550001240030118019000856023490220006900000000000000000000000000000000000000000
SPN1603Bacteria;Actinobacteria;Actinomycetia;Corynebacteriales;Corynebacteriaceae;Corynebacterium;matruchotii_nov_96.728%0000481004170152030093200436050502370000017019800240007500144037500009450023603220000406001460150046005100860451051002110039701150000111900460303000012000568025100008680026403806001480046540142000010100265011700007500227052700191030600324028300003300569079018006360025061060066100121000101001042001620302796001020002150130000000000000000000000000000000000000000
SPN161Bacteria;Firmicutes;Clostridia;Eubacteriales;Oscillospiraceae;Phocea;massiliensis_nov_90.343%000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000080000000000000000000310000000000000125709633994894951925850136394046194202114108601114944015025367793812413388221402626193891733234215
SPN1614Bacteria;Bacteroidetes;Bacteroidetes_[C-1];Bacteroidetes_[O-1];Bacteroidetes_[F-1];Bacteroidetes_[G-7];bacterium HMT911 nov_90.144%0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000001400000000000305300000000000040213362815221955842833722451364893100049362282825093993122912117452837914410881514226514125997194672725560996020873165173
SPN1621Bacteria;Firmicutes;Clostridia;Eubacteriales;Peptostreptococcaceae;Mogibacterium;neglectum_nov_89.855%00000000000000000000000000800000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000090000400006354825000000000000397574418536353299750105525142315819740534942619428383829924928820893915242452362894941085807712105314928806166514401216408175
SPN1627Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;maculosa_nov_90.061%000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000005990000000003004502801120737207681807219904223154429912145255384566638677516724256261128319407204441729910307923816737379002931714151602
SPN1638Bacteria;Firmicutes;Clostridia;Eubacteriales;Peptostreptococcaceae;Peptostreptococcaceae_[G-7];bacterium HMT081 nov_94.105%000989700003400002114370000258000425500001700081730000730910147752048701820660930652061701840531035118062500040015104006902801230922733070221001150135041126200252085040302058300100210305017824000227701215102663111004440151808093870300122841053088932637000025242001133133212000964637300002800000201095023214069000132045191200012073514358160000210000000000000000000000000000000000000000
SPN1643Bacteria;Spirochaetes;Spirochaetia;Spirochaetales;Treponemataceae;Treponema;pectinovorum_nov_87.755%00000000000000000000000000500000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000002000000000000000000005200000000000372520216532622132287476100791102449344664309383222172693332122410148686233318435164529542284511307540383276821542449750
SPN1650Bacteria;Proteobacteria;Gammaproteobacteria;Pasteurellales;Pasteurellaceae;Aggregatibacter;actinomycetemcomitans_nov_95.910%0154060330055653372084820101280205194177654158620780092403431950500560062704303859237805403760115119163060302000210047554415221352002968737179405533212800310149196120000260003225902886734590000001356377010700045003510088900014700132031026000930174447000112000074034002172400116021032267000331342930560000000001100001360171200160090000000000000000000000000000000000000000000000000000000
SPN1671Bacteria;Bacteroidetes;Flavobacteriia;Flavobacteriales;Flavobacteriaceae;Capnocytophaga;gingivalis_nov_95.992%000000000000000000000000000000000000000000255601274000002082023330000000010417500000080173167000000006682300000000682136000000260132027480000001905280564000040001516700001301901198000000250354230000009021741500000150000000220183516064652200004923370348294200014017600592180000000000000000000000000000000000000000000
SPN1682Bacteria;Firmicutes;Negativicutes;Selenomonadales;Selenomonadaceae;Selenomonas;noxia_nov_96.654%000309400000000033530000000001429000000000193000000000016700000000012000029000047300000000027000000000015930000200000119400001600005160000247000987000000000359000000000000000000019400000000044400000000013100000000043000000000770000000017210000000000000000000000000000000000000000000000
SPN169Bacteria;Bacteroidetes;Flavobacteriia;Flavobacteriales;Flavobacteriaceae;Capnocytophaga;sp. HMT332 nov_94.480%0000000012670000000007190000000009330000000005380000000005500000000010780000000001433000000000123100000000000000000000000000000000000000000000000000000000000000000000000000000000000000000189000000000440000000000000000000000000000000000000000000000000000000000000
SPN1692Bacteria;Bacteroidetes;Bacteroidetes_[C-1];Bacteroidetes_[O-1];Bacteroidetes_[F-1];Bacteroidetes_[G-5];bacterium HMT511 nov_97.083%00000000066300000000042700000000023900000000093200000000087800000000014700000000000000000000107900000000010280000000002910000000004390000000003746000000000595000000000738000000000153000000000013820000000001560000000000000000000000000000040000000000000000000000000000000000000000
SPN1703Bacteria;Firmicutes;Clostridia;Eubacteriales;Oscillospiraceae;Sporobacter;termitidis_nov_87.794%0000000000000000000000000011000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000180000000000000234294212396233375318891352604941011622327274326641155178525211142938312264072735558403964759293410853545438597182394
SPN1713Bacteria;Bacteroidetes;Flavobacteriia;Flavobacteriales;Flavobacteriaceae;Capnocytophaga;sp. HMT878 nov_97.028%17598665123786128931361104364040575717723544726701498935689012245053642366976021181061534918911666902751855274648323522645151300135190450959102810759501135953939340366173185907472553149914836630445749431350100324425642439281103672477251139696577161171518340312041445770132881008165132584355138397371153527326378422418011151787394578173811563301942127451676398406513101680910112011560273105317216490849671013627411562512588241341184052114057835333791144803135406405151179125738054163582373114113250517110493096302365119126644243895416133712431103081031774012718910229258001761121281433530600146281902386291121292618101533710000000000000000000000000000000000000000
SPN1715Bacteria;Firmicutes;Clostridia;Eubacteriales;Oscillospiraceae;Phocea;massiliensis_nov_93.103%0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000110000000000002205951021695480017829812903641180490001531890937285611210922553390353403957544407700133
SPN1727Bacteria;Firmicutes;Clostridia;Eubacteriales;Oscillospiraceae;Oscillospiraceae_[G-6];bacterium_MOT-153_nov_92.391%0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000009000000000000081175531016789421699629701891002638011801401363273157310551154730305676272761445125307900
SPN1739Bacteria;Firmicutes;Bacilli;Bacillales;Gemellaceae;Gemella;haemolysans_nov_97.787%3799197315517333852298571320047331641361128493625115256329358611252112427072286554569136913382221528445781362363022130215763454380133138033810056425573015881253531110159151175281323377138131568252845491694714131185110292341268230343536051021340171921410708501412710518179653185111541620257423929643793225673481667260399070616258132421501302314423222463766737642421710202684782112551533220093315352679832078344631973893490613556490386823874174254665181121210724892343348213551421125075840631761752242137861951900857147258876561684939833442105353633413648282851490941990000000000000000000000000000000000000000
SPN1740Bacteria;Bacteroidetes;Bacteroidetes_[C-1];Bacteroidetes_[O-1];Bacteroidetes_[F-1];Bacteroidetes_[G-3];bacterium HMT899 nov_87.959%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000400000000900000000000000190067205107640197236116301382021759591492472486351739060010227112131471054283789880144848066121932275
SPN1751Bacteria;Firmicutes;Clostridia;Eubacteriales;Oscillospiraceae;Hydrogenoanaerobacterium;saccharovorans_nov_87.397%0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000007000000000050000000000000095019028919625092135793712373538367018794312967273483141455015515283314613931808406841026351322202363641419400
SPN1763Bacteria;Firmicutes;Negativicutes;Selenomonadales;Selenomonadaceae;Selenomonas;sp. HMT478 nov_96.640%00010047500012580000733640002433101470010900001120000167000255939320047512017081032290170378049352992575842097004941030604486116014063521290097029210015736730000378130025754638877509700098102540222343600034616717013017335000101300091011800005073626795480000041157070997000059213017017000003160770140001536003744000005914191501800000160000000000000000000000000000000000000000
SPN177Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Lachnospiraceae_[G-5];bacterium_MOT-170_nov_92.453%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000002000000000000000000000000111928415217416113063292108588911289341881132611425712061207164207346158144166256316264229170395300165272209238
SPN1775Bacteria;Spirochaetes;Spirochaetia;Spirochaetales;Treponemataceae;Treponema;sp. HMT264 nov_83.534%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000487400000000000005030056000920326050015035800286021600008766550005461788001291102007546
SPN1778Bacteria;Proteobacteria;Gammaproteobacteria;Pasteurellales;Pasteurellaceae;Haemophilus;sp. HMT036 nov_95.501%0328000703500002700043130002500008354000000001370000000000121900000070031720000000008000000005035140000000025000290009000000000028000000000015000000500137000000000130000000002700000000032000057018201071214167640615630110439915021925455834122256664136628424371859953092880575151350618300206157160389426130000020000000000000000500000000000000000
SPN1797Bacteria;Firmicutes;Clostridia;Eubacteriales;Peptostreptococcaceae;Peptostreptococcaceae_[G-5];bacterium HMT493 nov_95.417%000087000000000880000000008200000000000000000002170096300000036100686000000127900930000006470022500000015002185000000169005670000003200211000000024006700000055900630000005190010500000029200650000000289002262000000900000000071008000000000000000006007000000000000000000000000000000000000000000
SPN1808Bacteria;Firmicutes;Clostridia;Eubacteriales;Eubacteriales_[F-1];Eubacteriales_[G-3];bacterium_MOT-163_nov_86.680%0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000029539561143316640134014128753598291820124479387718682001833839648887546112421187989003163714381199822228
SPN1819Bacteria;Firmicutes;Negativicutes;Selenomonadales;Selenomonadaceae;Selenomonas;sp. HMT892 nov_97.446%0007116000000005243000000000800000000004800000000497000134000001272000150000001190001800000246001270000044339001741310000502390010249700001631200951300007900015300000149725003751054120007242800451136218000950004811085600000002858591300001610000000021000660000015000421100008418004320700000000000000000000000000000000000000000
SPN1821Bacteria;Firmicutes;Clostridia;Eubacteriales;Oscillospiraceae;Oscillospiraceae_[G-2];bacterium_MOT-149_nov_92.292%000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000020000300005200018000400050000000138296185230552119596328420488186144149117110512251118125882524280290206516833888470279680150493271129810708041822061564494620
SPN1833Bacteria;Firmicutes;Bacilli;Bacillales;Gemellaceae;Gemella;haemolysans_nov_97.984%0000000002630000000008300000000000000000001300000000002160000450000135000000000033000000000112400000000025100000000024900000000021030000000001193005040000002005005005300007720064800000023500773000000181300000000018200000000039000000340014092100000070000000000000000000000000000000000000000
SPN1844Bacteria;Firmicutes;Clostridia;Eubacteriales;Oscillospiraceae;Ruminococcus;champanellensis_nov_90.086%000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000150000000000000394264580007190603786341464205001690071510186730000000143813261531489003107000
SPN1853Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Porphyromonadaceae;Porphyromonas;pasteri_nov_96.939%13137854907307184395738337884019359041963748145730338583122816540046954507317811926554912305702535536120601759470403573251901424271946314206116400128213453625015470910381061135817880351358286097651386804000223950036221493600013913200499277380021020630015230226100162810643900132021560072222339000244273508005738086000543552743051282748008910285304613561168800133504834287937846554490277730549041944116521050116375362603811352528203858503412536429280010850545701112049193374400204383210000000000000000000000000000000000000000
SPN1854Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;sp. HMT304 nov_95.492%0000000000000000000000000000000000000000000091100000000039800000000020740000000005300000000002600000000070500000000022100000000000000000001532000000000747000000000374900000000030880000000000000000000140000000002500000000034000000000000000000000000000000000000000000000
SPN1865Bacteria;Firmicutes;Erysipelotrichia;Erysipelotrichales;Coprobacillaceae;Catenibacterium;mitsuokai_nov_97.934%0000000000000000000000000000000000020000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000600000000020180000000000000441482773272282492661181122020459640197447209953215415148149209072922129444990175223671226775104941723210958316289
SPN1876Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Anaerocolumna;cellulosilytica_nov_87.657%000000000000000000000000002000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000139040000000000152162152177136131344552371064161751123344001433734442468627821132642754362056436712553631682873295343926244981210293104
SPN1885Bacteria;Actinobacteria;Actinomycetia;Actinomycetales;Actinomycetaceae;Peptidiphaga;gingivicola_nov_95.297%1280008970160026500376371341770077000840090022700037200900334002943713323600157002766825276700488000850000253170007400200000071924564100088300950357764460052800016700000110000820894002230000452240007900600363026002400079001000750008500110016000224000081600106440300060002737906001100025000000000000000000000000000000000000000000000
SPN189Bacteria;Proteobacteria;Gammaproteobacteria;Pasteurellales;Pasteurellaceae;Actinobacillus;lignieresii_nov_97.342%0000000090032707000120006220510001460001210420003810014000000210000250000000914100000113000133003400190000007009000400000000002000000900000000090000110210500000001400000000020700190004004900560039600272001229300484704403005990096214263040003650013731711004270655570000000000000000000000000000000000000000
SPN1895Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;brevis_nov_89.184%00000000000000000000000000300000000000000000000000000000000000000000000000000000000000000000000000000000000000000000004000000000000000000000000000000000000000000000001400000001200000000008039550000000000007951819185218567913151862235526998533121454731123820817029026322111949962653306242241072391816279337153526882117223551677223
SPN1897Bacteria;Proteobacteria;Betaproteobacteria;Burkholderiales;Comamonadaceae;Comamonas;faecalis_nov_91.803%00024100000000028900000870003760000044000302000001600066353500082328000880180003995800020499000485400021791000714200018552000068637000395180004334000015575000370520002818300047710021335270006160005851936000865350012429760000649330014723860000104320005000000000012000140000000000000008000700000000000000000000000000000000000000000
SPN1908Bacteria;Proteobacteria;Gammaproteobacteria;Pasteurellales;Pasteurellaceae;Haemophilus;haemolyticus_nov_94.908%017160000059000190000033100077000001227005700000056700122680000095000175800000258006690000043550200840000052000500000110060000000400101070000090090017500000346110017000001183504600000087130110000005019001090000041140218248000002125291091160000001020280508530000026323106965000004532950000000000000000000000000000000000000000
SPN1920Bacteria;Bacteroidetes;Bacteroidetes_[C-1];Bacteroidetes_[O-1];Bacteroidetes_[F-1];Bacteroidetes_[G-7];bacterium HMT911 nov_89.528%0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000040000000005050000000001700000000000002081093646811678138441581540454378931616016459834624497049221161830119761269358159499322674453096631116121
SPN1932Bacteria;Proteobacteria;Gammaproteobacteria;Pseudomonadales;Moraxellaceae;Acinetobacter;tandoii_nov_97.308%0000000120000010000000000002109000000010015500094114783299290003416845333271170001291420663501380002840152974700010188000000010074600000210023400000000000000000020110000000020460000000000000000000009000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
SPN1943Bacteria;Actinobacteria;Actinomycetia;Actinomycetales;Actinomycetaceae;Actinomyces;gerencseriae_nov_94.061%0031419600061000934404100200919117796027004900001470017001080490656610108307300195267693978076046064066280310001430018101141531940036012010107900000949050000106007423316409900160004200177287001160220182311600051000106000000000115331002070017700300831137366001040900000000000000000000000000000000000000000000000000000000000000000000000000000000
SPN1953Bacteria;Firmicutes;Erysipelotrichia;Erysipelotrichales;Erysipelotrichaceae;Solobacterium;moorei_nov_89.441%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000900000151510000000000000114206237252100355302114378115214238211165254152175196391142691984205413273243194013411291205433522741370476462599416220
SPN196Bacteria;Firmicutes;Negativicutes;Veillonellales;Veillonellaceae;Dialister;succinatiphilus_nov_93.738%0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000030000000000000000000000150000000000000442142592078576705624763782402795251719360874153328836240959902154345989212815828611022022
SPN1965Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Catonella;morbi_nov_97.699%090120460467700014989090733982103167000305393207175887318800046118801759890214300083615802086108966151563028601867990194477871285826024792711104102011103718637424316748259900507371729442703122431046160190912936213753584065683968803017963583005694330316043541185020826818871101002335330912570183828218247440030717341188056137722639616646325772814146612370805956234816565076785019911286162301532770410712612301442169060904862675201730118411412466247524370152172249300001417771031918888258064913213184122244322284067229250265343288015481121125210000000000000000000000000000000000000000
SPN1966Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Butyrivibrio;fibrisolvens_nov_91.546%0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000011017000050000000921069753678279613101251351372611161907401873616312024813917269519698011455474143037929683041868056249956261293132
SPN1977Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;saccharolytica_nov_93.661%00013140000000001152000000000442000000000977000000000200975181000000151083255000000118130813000000307641325100000017014327500000007010850800000006118000000002860000000000743598300000003661410390000000205456000000132231123700000010240000000001191002300000032013000000004411000000000000000000000000000000000000000000000
SPN1988Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;anginosus_nov_94.433%00000000079700000000016000000000000000000005400000000018600000000013010000000001600000000010960000000007740000000001010000000000350700000000016380000000006560000000004400000000000000000001175000000000373000000000190000000003900000000000000000000000000000000000000000000000000
SPN1999Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Lachnospiraceae_[G-9];bacterium_MOT-174_nov_91.494%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000260264507061124234417018051357211483466595708220801160436183145707143903730148118618361776981011800
SPN2006Bacteria;Firmicutes;Clostridia;Eubacteriales;Oscillospiraceae;Oscillospiraceae_[G-6];bacterium_MOT-153_nov_91.126%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000001800000000000000788003200001446306132910089000166341540900059500046803151521650177000120
SPN2015Bacteria;Proteobacteria;Gammaproteobacteria;Pasteurellales;Pasteurellaceae;Haemophilus;haemolyticus_nov_95.910%2800257000000170141300000600268000000002710000000230000000110000000320150119293110040016029119800300600000000001050004000001816380144881277013299000170281280105899526701450014957993782043000113603028020842709532170017013013137020346318059127723801562119000000128313813200828068012251342192968002727814603785061941046825372124582610000000000000000000000000000000000000000
SPN2018Bacteria;Proteobacteria;Deltaproteobacteria;Desulfovibrionales;Desulfovibrionaceae;Desulfovibrio;legallii_nov_94.343%00000000000000000000000000000000000000000000172000017700003700002190000297000000000610000950000540000147600004010000292600005000011200000000007140000525000069700005320000300000010990000380000620000064900000000000000000000000000000500000000000000000000000000000000000000000000000000
SPN2028Bacteria;Proteobacteria;Deltaproteobacteria;Desulfuromonadales;Syntrophotaleaceae;Syntrophotalea;carbinolica_nov_83.497%000000000000000000000000004000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000006015000000000000287003395286646178701771929394907911503452171260108927720016117232363175474241518139620814229438325041194
SPN204Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Kineothrix;alysoides_nov_91.770%000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000001201300000000000014677268702625778372387164217462258612513326968405921633860558469405161455318656211228497236284479
SPN2041Bacteria;Proteobacteria;Gammaproteobacteria;Pseudomonadales;Moraxellaceae;Acinetobacter;indicus_nov_97.938%0000000310000012000000000402709000000070181000589484261214340001812831731281380001201170712671230002700292967900093450000007119635000002000183000000001013000000013090000000000430000000000000000000008000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
SPN2051Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Alloprevotella;sp. HMT913 nov_97.751%00018815429531640000001571112385122000000336400000000464600000000090000000000020000000000000000001900000000004302900000085000000000230000000002600000000246301213372025800001207563484185540000071116433287000000012664432037000020714378000000000000000000000000000805748904000000000000000000000000000000000000000000
SPN2062Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;sp._MOT-128_nov_86.992%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000200000000000000000151400000000000001651351221156276690709167366731216973488126664399407406884613618430859169242069194336512156861814022466486608
SPN2073Bacteria;Proteobacteria;Gammaproteobacteria;Pasteurellales;Pasteurellaceae;Aggregatibacter;actinomycetemcomitans_nov_97.541%4920091216002376229131777388055712202941722319871113527838149006739582071335107226521200130379999811836015358784033513790244026916471111551102251318020351015118522250033912641962077811447032812841659985235461904065862670699041262050137056828442797311414326690765939285809722966425620400847139221768119158825200070861227284351938115514003546189124896210000168752720712866215609738817422534085131346265092159375292125053506145314311947101154363247235131192220451311522321011610483015282370171679251212020422280000000000000000000000000000000000000000
SPN2074Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Blautia;luti_nov_97.059%0000000000000000000000000012000000020000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000030000000000000000174500000000000017053234927220255586114325043929683124252515278301917123109654604468277383114267326425243223512159815225340786
SPN2085Bacteria;Spirochaetes;Spirochaetia;Spirochaetales;Treponemataceae;Treponema;sp. HMT236 nov_97.363%00000000010200000000010700000000037000000000138000013510000331000059700003700002258000000000789000034000016300009000011820000350000419000025000019900006700006820000700006480000410000114500008500008630000310000000001600003700000000090000000009000000000000000000000000000000000000000000000
SPN2095Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Eisenbergiella;massiliensis_nov_92.798%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000040000000000585163373887523777753318706362282766691702970341662208635814551219822433326377711504451541374450658437
SPN2107Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Coprococcus;eutactus_nov_93.133%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000104130003000000006041600107012010611170225706603530077392625946900103342104910411412922135810382500179
SPN2118Bacteria;Firmicutes;Clostridia;Eubacteriales;Clostridiaceae;Clostridium;sardiniense_nov_97.619%000000000000000020000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000600000000600000500029022000000000011003966031149349148326592115042466538223463395838093044520346861322017206435111392346277117901111281401512
SPN2130Bacteria;Firmicutes;Clostridia;Eubacteriales;Oscillospiraceae;Sporobacter;termitidis_nov_86.882%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000003000000000000000000000000000000000000000000000000000000000900000000000000400000000000000000000192229762442362481600883350949422258315297541289355100225269780129114240430743172611111762494825802640
SPN2136Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;sp. HMT066 nov_96.964%0502003200260504446340050270411980920040630584666600004829155028548167140296154246133164487220386087309322303800301230260624906240901057819334318770374131513251444455102941767111119373200046019393360344063216124318271411109721515910687817050721333022155090252805110511421080803322927280022026442226457824722825945014861171622477155181647502084512911554781738372867404800000000000000000000000000000000000000000
SPN214Bacteria;Actinobacteria;Actinomycetia;Propionibacteriales;Propionibacteriaceae;Arachnia;propionica_nov_93.148%0324307627006101263122176991800280753010296017001202018088000540000158077219801381718001098058421924315812914300018880110233915432903270040103971313695007800501860256154282911187004704173026625902624090017910844224422254910131009806531479703004300054015613941519154160015401153209291602216006050722798894550320032802021735815021800100195051114800005704722600111001004050384001560045021211199142137120000280000000000000000000000000000000000000000
SPN2141Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Catonella;sp. HMT451 nov_95.597%00000000016600000000038700000000019700000000092047004800001490136002200001300179001450000001520046000013401016607010000037401107001080000513016613042000020701440055000042104703905300005401552507500001880990260730000750923790790000215000000000125049000000000155000000050707000000000000000000000000000000000000000000000000
SPN2153Bacteria;Fusobacteria;Fusobacteriia;Fusobacteriales;Fusobacteriaceae;Fusobacterium;hwasookii_nov_97.783%093000000972000000000935126089000000000000000001610640000000600000000012280082064000000000000000000000000260000000000016950000000000009000000000000000001283000000000673000000010104770000000000000000000540005100000138000000000250000015073016200000000000000000000000000000000000000000
SPN2163Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Blautia;luti_nov_96.218%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000003000000060030000000027190000000000002543462031948965754920325822814047141218491277178982161821192694041332029427130923552309155314276236435301466298120
SPN2172Bacteria;Proteobacteria;Gammaproteobacteria;Pseudomonadales;Moraxellaceae;Acinetobacter;variabilis_nov_97.113%00000007000009000500000001704000000000168000441167524141240000261179411432103000121125054275880002110271375400087080000000129621000008001620000000007000000013090000000000360000000000000000000077000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
SPN2182Bacteria;Proteobacteria;Gammaproteobacteria;Pasteurellales;Pasteurellaceae;Aggregatibacter;sp. HMT512 nov_95.297%239773009120532234003312300056190811171963225280844545414847914496082000631648246701548804201443811930045600366513615340043438053311115198911559887386267501768420519380001216345598624133043150001035465960228781193844111332312471310170360751822220023111611780134493190108130922114045113159750066741623666815500361622471273116811417545351914592496140781665367559151625156842445461422220617014274780554717115763586355659593450830219229703254534873510822375232962375783181298418712433438991400000000000000000000200000000000000000
SPN2201Bacteria;Proteobacteria;Gammaproteobacteria;Pasteurellales;Pasteurellaceae;Haemophilus;massiliensis_nov_95.918%0001200000000007000000001411000000001118000000000000000000580000000051143300000001390535001000000516000000010000000000077250821000084359054118000060560000000041210000095541261410000520300233515790000131117900004500771957940110107000068698606920144470002481243553836724743300000000000000000000000000000000000000000
SPN2210Bacteria;Proteobacteria;Gammaproteobacteria;Pasteurellales;Pasteurellaceae;Actinobacillus;indolicus_nov_97.741%0000000000000000000000000000000000000000000000002860000000007530000000002188000000000160600000000018700000000053600000000096300000000021500000000002200000000092000000000220000000000175000000000000000000052300000000025540000000027500000000000000000000000000000000000000000
SPN2221Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Oribacterium;sinus_nov_91.213%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000142800700000000002803672382232513952092162964002271319916744840023238319196891076693443104151291441825530827919624628652416761272200
SPN223Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;stercorea_nov_95.885%000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000003073000000000000027683761922411278246138462491580148137240441553512315179487881022435146302331661202741081796710512225123
SPN2233Bacteria;Actinobacteria;Actinomycetia;Corynebacteriales;Corynebacteriaceae;Corynebacterium;auriscanis_nov_97.436%3620000022000001200019001212000003680000000006400011000000000210000240000000003900000000000000000000190009100000285006000002317028013001300044000000000000000000000000000000000000000000451360008012144661751356702001971811711739327133170012311339287928410330053788530230106100000000000000000914000120231400600046012016204200
SPN2241Bacteria;Firmicutes;Clostridia;Eubacteriales;Peptostreptococcaceae;Mogibacterium;timidum_nov_94.990%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000062700000030000039079975943928322882398315758712861781790804484464734327078126931504791091955456184495936313623814082
SPN2250Bacteria;Firmicutes;Negativicutes;Acidaminococcales;Acidaminococcaceae;Phascolarctobacterium;faecium_nov_89.505%00000000000000000000000000500000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000003000000300000000000836310000000000003182117504613970492934101268811685364746744310715949186474941103322438341257217633830350634410483863125422831737011281595
SPN2254Bacteria;Actinobacteria;Actinomycetia;Micrococcales;Dermabacteraceae;Brachybacterium;nesterenkovii_nov_97.419%61220000600080000090011000016097001400300019020012000400000000331000000000900000000016003022000000000220000400002200300071000500000235000000000007060000000000000020000000000002131663801191095654113335267931401259610022039713681787416110114313702265192204305491424114600145012200000000000000000000026000009509400091800
SPN2265Bacteria;Firmicutes;Clostridia;Eubacteriales;Christensenellaceae;Christensenella;massiliensis_nov_85.010%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000003000000000000000000000030000000000130000433500039805490011123001172103013190050013004441052838000020863
SPN2275Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Blautia;luti_nov_97.059%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000005000000000000000000000000000000962392012702514204007515613516503326244610912150472194120646794272472931681222867425156229617501260111203346102
SPN2286Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;nanceiensis_nov_90.361%510811917872120168015041136520294201254329827229531016740036643150159317132348000165412192615200430362115413663652254958150726321040302117636038887263291739590879157539529810052471363649801522016132319731550193926200222064104321590958276874145601731463749952027341297362210511803185040232431449006011962245600183149740264039372120730181619364730623675580169123454228401411295817053781237076519233262503103611692171704768037920994929648913822003382120013426730337701001536269616601434149320563259396302041601602769240150147287726300000000000000000000000000000000000000000
SPN2287Bacteria;Actinobacteria;Actinomycetia;Corynebacteriales;Corynebacteriaceae;Corynebacterium;doosanense_nov_97.629%551400501700000000010040000090239009210000059000500000000041000013000000000165070000000000010800000270001950000270012170000161002200001800043110000000000000000010000000000001900000000000461281802555642774136142781027811681711853230272193584062100813072671299135261345812333007871400008040920000059000842512127808100121210014196190
SPN2298Bacteria;Firmicutes;Negativicutes;Selenomonadales;Selenomonadaceae;Veillonellaceae_[G-1];bacterium HMT155 nov_82.903%000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000003000000000020400000000922500012002000020000000000005000000000000000000000000000000000
SPN230Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Catonella;morbi_nov_97.292%0000290300000000080200000000000000000001170000000001520083000000147000000000000000000040000000000147001050000000200000000000334000000000121000000000113003730000001470012600000021000000000410000000006700000000000000000001500000000032000000000000000000000000000000000000000000000
SPN2308Bacteria;Firmicutes;Clostridia;Eubacteriales;Christensenellaceae;Christensenella;massiliensis_nov_85.185%000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000021000000000000103031195240141090029217320659285473513051156147153822498065461877071992236772748470033338
SPN2318Bacteria;Firmicutes;Erysipelotrichia;Erysipelotrichales;Erysipelotrichaceae;Holdemanella;biformis_nov_96.774%000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000002000000000000000000000000000000000000000000000000000000300040000000000005000000170000000000000801731191394518038946246501955975345116181151261316360318454994937309229269782188529265813232253197820617
SPN2324Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Muribaculaceae;Muribaculaceae_[G-2];bacterium_MOT-104_nov_88.508%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000004000000000000000000000500000000000317074165013924053537726021315528503981312120036413610919216128238411101922833332401443023138322059356
SPN233Bacteria;Proteobacteria;Betaproteobacteria;Burkholderiales;Comamonadaceae;Brachymonas;chironomi_nov_93.621%000023500001420000600000011800000000047500000000042200993072700744014400725057300433011100144012500593050025202070013102900113601036005710133005860292002260116008540670032501950013102200790016004220289001074029001070286008180270049081006861041330013049003542796000000000023001402000000040036000000000200213800000000000000000000000000000000000000000
SPN2333Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Blautia;obeum_nov_96.753%00000000000000000000000000800000000000000000000000000000000000000000000000000000000000000000000000000000020000000000000000000000000000000000000000000000000000000400000000000000000000300002300000000000090388209143204739255208116209166180179107318160244605341555079509243322221134362143524350137174178218119195265319119
SPN2342Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Coprococcus;catus_nov_97.098%000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000600000000000009412441385137338330276184591743801191131369519818294242181213333214291237222205258249528306280402231557173342140162
SPN2351Bacteria;Bacteroidetes;Bacteroidetes_[C-1];Bacteroidetes_[O-1];Bacteroidetes_[F-1];Bacteroidetes_[G-3];bacterium HMT280 nov_89.876%0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000400000001100000000000600000000000001319113048335562680131371631731336118335777957013915110327106901787566367165520939196581155548235
SPN2357Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Rikenellaceae;Rikenella;microfusus_nov_84.091%000000000000000000000000000000000000000000000000000000300000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000003000000242112000000000002410150724702293015435252562659123343830484922438161585641584117889181275110441374133332241189218243598236632253
SPN2363Bacteria;Firmicutes;Clostridia;Eubacteriales;Peptostreptococcaceae;Peptostreptococcaceae_[G-7];bacterium HMT081 nov_96.414%000000005840000000003514400000000000000000062170000680000390000060002454600000220004390000057000314288000052000130300000048000106342000037000374980000310004915600001680090161000018300002130000547005653645000028400124305190000000002600000000610000000007000000000000000000000000000000000000000000000000000
SPN2373Bacteria;Proteobacteria;Gammaproteobacteria;Pasteurellales;Pasteurellaceae;Haemophilus;paraphrohaemolyticus_nov_96.945%3190000152200064000024650001790009107120037075000013628013000000000001400058100700000011380000000019160000000018070000000001200000600282300000001800280070002200000460003410410000000680000000003420000030006701236002269092230646194381236828053524121723751716152588016906165219985015120230000000000000000000000000000000000000000
SPN2384Bacteria;Actinobacteria;Actinomycetia;Corynebacteriales;Corynebacteriaceae;Corynebacterium;durum_nov_92.727%30000250136000110000231190007805103010415213900000029122350410017100018601130540490032110000000038003947061072700000175514600000062087000000000104083021021990969193393100032007983282500780000040000290000019014700022400021900090890002700028006210413523074001625503727470003201421576602632520791914100382100000000000000000000000000000000000000000
SPN239Bacteria;Bacteroidetes;Bacteroidetes_[C-1];Bacteroidetes_[O-1];Bacteroidetes_[F-1];Bacteroidetes_[G-3];bacterium HMT365 nov_87.860%000000000000000000000000001100000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000009000000040000000000016120000000000009930491173201212542538896069641645854677923020145313985167176287681281728423081341747253898157
SPN2395Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;stercorea_nov_90.650%300000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000014000000000028105000000000006045643972954049333849085973506116525426649410012995141909391817215944771825489480711811391313801999341
SPN24Bacteria;Fusobacteria;Fusobacteriia;Fusobacteriales;Leptotrichiaceae;Leptotrichia;sp. HMT212 nov_93.043%1500364232973751585636147965727253133989306154033348442952708963614280259227862517432728433423340257925374281264221941053008480358330281662351522833077444700685487011162860530429202599119423579521201460316641319604019231907570861621936431128372186457045137252710146482989346314442375252232353004124343357703057287065254213621137921565707822434157838961190120574997141688151112342591456413547803441845432229552711745171202121662921333366643622777336605752314302957643234933256230619641615036378401415196205040151528291327697993222431273781321998344929360266290871981254835834561537333313820666373391146114041452643102851646136249720649222736683123355101084913695761930610691211416131524333631790798326723111201396234883090000000000000000000000000000000000000000
SPN2405Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Lachnospiraceae_[G-3];bacterium HMT100 nov_95.407%0000186000000000548000000000447000000000210000000000408000000000149000000000257000000000467000000000344000000000650000000003640000000001270000008490095000000515006600000011560075000000186500770000000006600000000062000000000280000005300069000000000000000000000000000000000000000000000
SPN2414Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Anaerobutyricum;hallii_nov_97.053%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000002300000000072500000000000015121519212247372274585231802221215587259175145138861071242075612844946411237616421445852939409225280206264185302
SPN2426Bacteria;Firmicutes;Clostridia;Eubacteriales;Clostridiaceae;Clostridium;saudiense_nov_96.970%000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000022000000000010000012000000002527248831219247723630134310670488901282125126243033470916361429138644890231484028730368
SPN2437Bacteria;Firmicutes;Clostridia;Eubacteriales;Oscillospiraceae;Ruminococcus;flavefaciens_nov_93.723%0000000000000000000000000020000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000900000000000000000001413000000000000001240001200000663204000101360011730600748048303021432422119510055793631760
SPN2446Bacteria;Actinobacteria;Actinomycetia;Corynebacteriales;Corynebacteriaceae;Corynebacterium;durum_nov_93.522%2771971200023000049439000541069000532209000281130002914150051146100063266030260460009500210000000290005256001686766000000060000000000340000017800250023240594000000000531550006730000024008355150000022076000190002300028000001100012156000103900602736052222014000104210027100270026484616371800012350000000000000000000000000000000000000000
SPN2458Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;copri_nov_93.483%0000000000000000000000000060000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000023000000000000004379535116920429454016586472822165912976385731202024485066410169425513477931109038161027013060651
SPN246Bacteria;Firmicutes;Erysipelotrichia;Erysipelotrichales;Erysipelotrichaceae;Solobacterium;moorei_nov_90.928%000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000050000000000780000000000008016547757119719790129688049432171021547322020890342454826218519325183170285281257201590136258190248162127
SPN25Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;sanguinis_nov_95.758%66900002508002700068106007300034350160031000080090000001340000000081303100000030640000000014210300000029010000000001101400000002460000000051001800000000000000000008000000073080000000450430016100275832879391000000764540189219000000136881957710000028060001154000000000000000000000000000000000000000000
SPN256Bacteria;Spirochaetes;Spirochaetia;Spirochaetales;Treponemataceae;Treponema;succinifaciens_nov_86.992%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000050000000000000645328600140763499153702464676350111679180350999741106251021207198401178281818600784253
SPN262Bacteria;Firmicutes;Clostridia;Eubacteriales;Oscillospiraceae;Sporobacter;termitidis_nov_85.837%0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000070000000019423000000000000100262190281214833151701412404609530834314137111845857001589517930511031813195030131454064237
SPN271Bacteria;Firmicutes;Negativicutes;Veillonellales;Veillonellaceae;Veillonella;sp. HMT780 nov_96.634%0500000000000000000005000000000300000000060700340002300670070000201136000019003800020040000000000000000000000002502200000001702100400008070000000000000001706803300000100189221000002830000004258961654052801530272215207211292064615130951512900490031402089300000000000000000000000000000000000000000
SPN280Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Dorea;formicigenerans_nov_97.694%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000006000000003000000000000000511795722914429017777119181611488010621891468010896456058032414916313173524221894471323212741861076414855
SPN285Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;stercorea_nov_96.091%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000017170000000000009466653316642133136771071691961401860191363064078174731212012765559782500255158771191124825161115547155
SPN296Bacteria;Bacteroidetes;Flavobacteriia;Flavobacteriales;Flavobacteriaceae;Capnocytophaga;endodontalis_nov_95.560%0353619130000024479501124105600000964890034025130014005276130494878800000821339013307959228861000116722401216297888251700048230352413131341800014913730305470614935300031045402499986113030002352021224427593200002060208076944489260011944803047352616936000122106011554875408000018304522525615337003002220494421640200087730300793348013400003533075249159926074927054457102712099775434368043420060150972421413019075073037643690001592120000000000000000000000000000000000000000
SPN297Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;micans_nov_92.008%0000660000932000038400009850000230000106000000000810000194000013000050000090000520000000001100000900003800003900001170000230000140000900000000068000094000049000013000004610000130000043800002800001300001241000036200008000000000700000000017000000000000000000000000000000000000000000000
SPN3Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;sp. HMT942 nov_95.492%0000000000000000000000000000000700000000090000000005026000000033665440000002522880000000000000000000000000170015321000000004251100014000090000000000000000036057150000501295650000002660000000420018804212771800200310305671553990030445580774170247240000950000000000000000000000000000000000000000
SPN306Bacteria;Firmicutes;Clostridia;Eubacteriales;Oscillospiraceae;Oscillospiraceae_[G-2];bacterium_MOT-149_nov_91.667%0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000016000000000000001652721348251113882463793143319116521311534772687305393127114128143228013414326465179120171145743136086
SPN317Bacteria;Firmicutes;Clostridia;Eubacteriales;Clostridiales_[F-1];Clostridiales_[F-1][G-1];bacterium HMT093 nov_92.457%0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000030000000069010911170011260045182140822813648970946476011117214871109267339678121940459999444
SPN328Bacteria;Bacteroidetes;Flavobacteriia;Flavobacteriales;Flavobacteriaceae;Capnocytophaga;gingivalis_nov_97.059%00990000074000068000001098900900000558300000000350000379000005135001630000010640097000003352400190000017569004200000300079000000140030900000880020200000206008000000210025000000190077000000210001100000031001330000093600186000005250028000003160025000000190000000000000000000000000000000000000000
SPN337Bacteria;Firmicutes;Clostridia;Eubacteriales;Oscillospiraceae;Oscillospiraceae_[G-2];bacterium_MOT-149_nov_93.347%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000300000000000000000000145310000000000001861804391802211281622216361862401561822022066112751210661992041001762311134788112531116690170131332098169
SPN346Bacteria;Firmicutes;Clostridia;Eubacteriales;Oscillospiraceae;Oscillospiraceae_[G-2];bacterium_MOT-149_nov_87.344%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000040002900000000000095941805349140630551626520151186106932321899217615901963827714350128189261138032662919673122118304161
SPN35Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;maculosa_nov_88.776%000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000160000000000000635064614865390212722541212021744997645705018214311233110018623445518372105308407293113225247380123336861
SPN351Bacteria;Firmicutes;Clostridia;Eubacteriales;Christensenellaceae;Christensenella;hongkongensis_nov_82.004%091000005980193053006006570379067000009205890000000525016404100310012205803490035006609303950074000000234003700301110177000400059602770560600221001170239032300180029901230249000004501450870007900190540661009900250131016170099002190169039800820082089090000000380800160000001040029010000026003000000000000000000000000000000000000000000000
SPN355Bacteria;Firmicutes;Clostridia;Eubacteriales;Oscillospiraceae;Ethanoligenens;harbinense_nov_87.582%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000500000000000000000000000022185059646110127252885312299302187960673388249584103618151956108262214105373161910407216
SPN363Bacteria;Actinobacteria;Actinomycetia;Actinomycetales;Actinomycetaceae;Peptidiphaga;gingivicola_nov_95.122%07700440018400021001705202000390047000001383001740070004800213006500202390032035460070181004900002779929400360014000498007100380005000000350089101007200000511530052009700000000066000800000150007100460000022700039001100000010600003203974035000000100008000031089100120000110000000000000000000000000000000000000000
SPN370Bacteria;Firmicutes;Clostridia;Eubacteriales;Oscillospiraceae;Acetivibrio;straminisolvens_nov_83.721%000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000400000000000000047200111061920173141454704754813048120511218600551452301711599703466530181620279353
SPN380Bacteria;Firmicutes;Erysipelotrichia;Erysipelotrichales;Erysipelotrichaceae;Dielma;fastidiosa_nov_81.162%0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000011000000000000000041712000000835892270060127560203711000041711305204000016189990
SPN391Bacteria;Proteobacteria;Gammaproteobacteria;Pasteurellales;Pasteurellaceae;Actinobacillus;minor_nov_93.865%28141401224936103161019803301241922826913050648055025410012699186312620191560045310153666107053306916026149913820109132311060107931566117176302483725702100510033206710494325961436094017602311969610000164322289000009000104800742821611632442137802513490251302141532231121901973530699301519846123209141371519164402553421043370812211131568099110830520170501391549285259049082822390210361718478920753525628918119129617525729561144152605815412920811072757923002191478111655462532093064965243100000000000000000000000000000000000000000
SPN392Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Lachnospiraceae_[G-8];bacterium HMT500 nov_97.684%0000000008400000000080000000000000000000082000043000041000000000294000047300000000094000024700007000006840000303000041300009600002600000440000647000023400007200002630000109000038100004800002360000245000000000520000000000000000000700000000000000000000000000000000000000000000000000
SPN399Bacteria;Lentisphaerae;Oligosphaeria;Oligosphaerales;Oligosphaeraceae;Oligosphaera;ethanolica_nov_83.592%000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000041000200241900000430029553915506032339066680224910514361541887166430126286229392924927254284
SPN408Bacteria;Firmicutes;Clostridia;Eubacteriales;Oscillospiraceae;Oscillospiraceae_[G-2];bacterium_MOT-149_nov_87.321%0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000005000000000000000000000060014351478324026537052181535251334428970121512002086437951911233349123208248255888099028
SPN416Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Bacteroidaceae;Bacteroides;heparinolyticus_nov_95.885%000000000630000000001820000000002100000000015600009500007300007300003170000000000000010800002470000000003170000530000379000000000181000000000292000010400020679000030300021106100001100007000004600004590000000001100000000000000000000000000000000000000000000000000000000000000000000000
SPN423Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;timonensis_nov_88.415%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000111040000000000000119128228263223159120695361807419818911041612604334795892122137921331654016279831439477104681610183
SPN432Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Tannerellaceae;Parabacteroides;merdae_nov_86.613%0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000001010000600000000002890000000315613525301721950101522871930107004001310144037620825003527404601164
SPN44Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Lacrimispora;saccharolytica_nov_94.375%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000050000000090500000000000112111160188373310230432237381926316929367173125374101206233016531588151399137496152446212423105275302206250117144
SPN441Bacteria;Bacteroidetes;Bacteroidetes_[C-1];Bacteroidetes_[O-1];Bacteroidetes_[F-1];Bacteroidetes_[G-3];bacterium HMT899 nov_87.680%0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000059244142041226311552000424170186574000254101050171603027518024052320554814701524
SPN448Bacteria;Firmicutes;Clostridia;Eubacteriales;Clostridiales_[F-1];Clostridiales_[F-1][G-1];bacterium HMT093 nov_84.829%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000002600000000000022032160017422001343295173081402380427626002200517595129203866474303418028694
SPN458Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;oris_nov_89.069%0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000034124442573122820221302218546311323244397009285773016712921632334182068456091561381978022985
SPN467Bacteria;Spirochaetes;Spirochaetia;Spirochaetales;Treponemataceae;Treponema;succinifaciens_nov_85.455%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000013000000000000018325013515329129832542774113266839862385155820957406218911970780108914194929729933125356641
SPN470Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Tannerellaceae;Tannerella;forsythia_nov_97.516%0000314006920123100004760015250139000002780017808160000559001337070900001060010401160000640054017900006060057020000820040177000025400279046900003820013604210000138007880334000016005304980028102300000206003180219001803010026046100640035800003360017902790000131000010800007000000000000220000000000000000000000000000000000000000000000000000
SPN475Bacteria;Bacteroidetes;Flavobacteriia;Flavobacteriales;Flavobacteriaceae;Capnocytophaga;sputigena_nov_95.117%04556157900000017203818956000000116046642210000002090340311000000490157213822000000420520837500000020068392335000000359030411600000000537078582140000002405516466200000028031271551000000640276130270000001670401823130000003601129655400000066015422170000000116202658751000000345017545000000285011443100000019047715000000014076535000000270000000000000000000000000000000000000000
SPN476Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Roseburia;faecis_nov_96.868%0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000006000000000030000000009170000000000001635032839219161103541862234105815410824948832634700124732343614310204802149262647501390
SPN484Bacteria;Firmicutes;Clostridia;Eubacteriales;Oscillospiraceae;Oscillospiraceae_[G-2];bacterium_MOT-149_nov_91.892%000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000002000000000000000000034000000000000252010541374525136161894123396109495925822304910616525124201835413089106426239461144224524969235112
SPN494Bacteria;Firmicutes;Clostridia;Eubacteriales;Oscillospiraceae;Phocea;massiliensis_nov_90.323%0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000044024448562003250021141074192640100304742025302923409205130029809729566036034
SPN50Bacteria;Firmicutes;Clostridia;Eubacteriales;Oscillospiraceae;Paludicola;psychrotolerans_nov_87.578%000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000050000000001016130000000000006045661904001572313015964313536187147348492311210164264364512331128107932273465961265490748123818760
SPN503Bacteria;Actinobacteria;Actinomycetia;Corynebacteriales;Corynebacteriaceae;Corynebacterium;durum_nov_92.757%18202700050004180000858410001470000242200017300000204000493300002100028000007000000001011400047000000000000000000032000003650001506100193190000000097630004980500000001750000000000046000700017000000000592100013000046120001200070691001120800009224290151900000000000000000000000000000000000000000000
SPN513Bacteria;Actinobacteria;Actinomycetia;Corynebacteriales;Corynebacteriaceae;Corynebacterium;durum_nov_93.293%0000002650140115000001700038802300016787130183160000422900680008501700101200080000011000005496701500180000685000000081097000000000330503900002072442400000004836258000010080468000000000020000000205360000000039130000000028210000013401430000742031162227651712355026000000000000000000000000000000000000000000000
SPN521Bacteria;Bacteroidetes;Bacteroidetes_[C-1];Bacteroidetes_[O-1];Bacteroidetes_[F-1];Bacteroidetes_[G-3];bacterium HMT280 nov_86.542%000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000023000000000000236664230012251403451152010131653234298734132433831175401922336845425013932359376920180196
SPN530Bacteria;Bacteroidetes;Bacteroidetes_[C-1];Bacteroidetes_[O-1];Bacteroidetes_[F-1];Bacteroidetes_[G-3];bacterium HMT280 nov_89.278%000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000003000000000000000000002604730842044515020821925119674002008697082711163605300723030206933940414633246119142
SPN534Bacteria;Firmicutes;Negativicutes;Selenomonadales;Selenomonadaceae;Selenomonas;sp. HMT892 nov_96.832%0001036263260396000001287034307440000007600349000007133003220000020139238020100000001842095258001000083612670000000252131110000000323101204800000033914630000000045500000000025700500000021415881371001000031520308170000000809441750000000726582031204000012790018000001737006000000211200130000006173300000000000000000000000000000000000000000000
SPN541Bacteria;Firmicutes;Clostridia;Eubacteriales;Oscillospiraceae;Fournierella;massiliensis_nov_90.208%0000000000000000000000000040000000003000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000008070000000014005000000000005423482149181158224786037928010195895353502428908248958534311273345391271095512914224064255744
SPN552Bacteria;Firmicutes;Clostridia;Eubacteriales;Christensenellaceae;Christensenella;timonensis_nov_85.391%0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000842421320802008645521077049433055485197193122021414226966012061815190349811860163243
SPN568Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;sp. HMT317 nov_97.947%507733100018894571466241193017525181271926891108145631561620451750146658298421657420070486762389048154910164310246390928780993249452096001401211245794232121565703835895575311626155136699009736177223366622164145160000131111741016144835607900676175621294357283302340202019132954154416460023307930042863152464392170297901558672113715163403090157002501289111267335348371780132095200450415035221029304316388454791641691101740128140348199657312300141273511793234007300363363604814617604000841685014327331079000000000000000000000000000000000000000000
SPN569Bacteria;Firmicutes;Clostridia;Eubacteriales;Oscillospiraceae;Phocea;massiliensis_nov_89.241%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000127290000000000003612222530261089224601849896132101921072460259769113204191213204157148065240120287130632258520125157
SPN577Bacteria;Actinobacteria;Actinomycetia;Actinomycetales;Actinomycetaceae;Actinomyces;sp. HMT169 nov_97.189%790600015630006600001698460141700001600084100005470001150056063085690573090290012000000005009391900000000082000003700700700000026000006000000000080003680000012020181070001000300000001013900000000010000085170200000400191940001100021393153807014240233180000000000000000000000000000000000000000
SPN584Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Blautia;faecicola_nov_97.071%0000000000000000000000000000003000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000010000000000130000000000000588520854781591457813428115698271146571872501337505125858313514215517124613086132471621842372231510
SPN585Bacteria;Fusobacteria;Fusobacteriia;Fusobacteriales;Leptotrichiaceae;Pseudoleptotrichia;sp. HMT221 nov_91.164%3341500400130009005007002611679013701140637126350120012405026000000000210770009004546570520038004202029705607100000050000000000000016051926261370919408223761970528011000141326700122006151912700704146011926316150283282082536315835201216100631781651470091643331141453231205644402351016049406333864227296875978773537696731149736631401394450000000000000000000000000000000000000000
SPN597Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Muribaculaceae;Muribaculaceae_[G-1];bacterium_MOT-129_nov_81.136%000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000050000000000000000930036516000002632611213241601650001031611471442992551990004324925211391120
SPN606Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;copri_nov_95.732%0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000150000000000000000000140000000000000014216553220180176060333550351144244603771252379349322223255332035492166002904342185925
SPN61Bacteria;Firmicutes;Clostridia;Eubacteriales;Oscillospiraceae;Oscillospiraceae_[G-7];bacterium_MOT-154_nov_91.863%000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000392207000004580890595172862028791401011310015000012102223129461837933508727064760
SPN617Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Anaerobutyricum;hallii_nov_97.689%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000115000000000000020215136919513531986708753116019355938202106102133668613412032103532382457594148591242657237127
SPN629Bacteria;Firmicutes;Clostridia;Eubacteriales;Oscillospiraceae;Flavonifractor;plautii_nov_93.789%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000008000000000000241521341731041632371101352018814516154421641631262187113978518378102123568349128173229981295212410394102
SPN638Bacteria;Firmicutes;Clostridia;Eubacteriales;Oscillospiraceae;Sporobacter;termitidis_nov_86.052%0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000700000000000000000000000000000000016490143361241361442325699375216113348815223748139981841061410114623258433250237907934655116797227
SPN646Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Lachnospiraceae_[G-4];bacterium_MOT-169_nov_95.597%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000141372663154189139208220232181371071199713689010066103132481729531727187135863362327621719413617567
SPN656Bacteria;Actinobacteria;Actinomycetia;Kineosporiales;Kineosporiaceae;Kineosporia;aurantiaca_nov_79.060%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000007000009016591000000006011050000000071547008300000123
SPN664Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;sanguinis_nov_96.371%333500021146129800174000233520290033800079269399400270004136904700011402519120200601007026470577010023004230078701204900231350258000000992434240508705521114101030000101105633650407241340327000006023016050008000121040000037809732600000202082012677191236482531378302167203502692136778734042891122030735266501177364626075475210762086102456010432521230937649380459069023057770000000000000000000000000000000000000000
SPN665Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Blautia;glucerasea_nov_95.378%0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000002000000000000000000000000000000000000000000000000300000000000000000006000000000000012013013592641863051967713043069751567318231416668143321881191295537192231515910214012418984236107101
SPN675Bacteria;Fusobacteria;Fusobacteriia;Fusobacteriales;Leptotrichiaceae;Leptotrichia;sp. HMT225 nov_96.957%0000000000000000000000000000000000000000000034000015000000000410000266000000000701000000000260000329000045000041700009500003520000000005410000669000000000344000044000037700002600002800005800000000000000000000000071000013000061000000000000000000000000000000000000000000000
SPN68Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Anaerocolumna;cellulosilytica_nov_90.476%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000001200000000000016216659911844148544841827434716517713454010019213718046053310621104019273904238380724920259519
SPN685Bacteria;Firmicutes;Clostridia;Eubacteriales;Oscillospiraceae;Oscillospiraceae_[G-2];bacterium_MOT-149_nov_86.858%0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000260000000000003303115462461521356980938579628518115926481651101052162601838233359774951011125816111333356205
SPN692Bacteria;Actinobacteria;Actinomycetia;Corynebacteriales;Corynebacteriaceae;Corynebacterium;matruchotii_nov_97.358%05500000000003980000000000000000000000000000095000000000629000000000309000000000680000000002800000000001180000000002230000000008000000000041000000000939000000000348000000000560000000000000000000000000000015300000000080000000000000000000000000000000000000000000000000
SPN700Bacteria;Firmicutes;Clostridia;Eubacteriales;Oscillospiraceae;Oscillibacter;valericigenes_nov_93.333%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000004000000000000000000000000000000000000000000000000000000000000000000000000000000011000050000000063712299491685920033056101951906548742961300134771325937183016716046514723016868254316145218149
SPN704Bacteria;Proteobacteria;Gammaproteobacteria;Pasteurellales;Pasteurellaceae;Haemophilus;sp. HMT036 nov_94.286%113410000007421501420200090100072000061641122600000024070460810512590005570010029101301500000180240000064046000000390060008000000040000012300030000642016001300020000013700035000008000211105071000017560001111480023214360191546421017001301298322131972085070934705094828552256974010770508435910626503194000000000000000000000000000000000000000000
SPN710Bacteria;Firmicutes;Clostridia;Eubacteriales;Oscillospiraceae;Oscillospiraceae_[G-2];bacterium_MOT-149_nov_88.565%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000595413264261411934811070029356302643913710701124913847722083366161512444369470167770118115105
SPN721Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Paludibacteraceae;Paludibacter;propionicigenes_nov_85.685%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000002860360000005400020520000056001111147000004301440
SPN731Bacteria;Firmicutes;Clostridia;Eubacteriales;Oscillospiraceae;Oscillospiraceae_[G-4];bacterium_MOT-151_nov_85.010%0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000002200000000000001900280115039070100530233291293008400100079111133063700110064453000
SPN739Bacteria;Firmicutes;Clostridia;Eubacteriales;Eubacteriales_[F-1];Eubacteriales_[G-1];bacterium_MOT-144_nov_86.299%0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000001821040000000000244279292001618774982361419261340605024353791799915562165199668594622021041146913314136191719
SPN749Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Blautia;faecicola_nov_90.664%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000003410000800001200000800000185400022000018410
SPN757Bacteria;Bacteroidetes;Flavobacteriia;Flavobacteriales;Weeksellaceae;Riemerella;sp. HMT322 nov_97.942%990054776433035200000061363563468132400500017869857534066169200370013562741103274537001680090013221795125001740028101267701254009600158026595173498007000145095832114390000000981383837007460000143023809100720002100700141054000355009905631317039700858009901688533610014450016301666564590032000188017681540450024100121010379283600420010662114158840000038072151640070019053499380032003903429236000000000000000000000000000000000000000000
SPN759Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Kineothrix;alysoides_nov_91.770%0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000800000000000013193308136428701901211604032253123202958056027125602184586068337491361313006333641410153
SPN768Bacteria;Bacteroidetes;Bacteroidetes_[C-1];Bacteroidetes_[O-1];Bacteroidetes_[F-1];Bacteroidetes_[G-3];bacterium HMT899 nov_88.912%0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000067031039201320900155522201085663900015739002124282012404029332023229350240
SPN78Bacteria;Firmicutes;Clostridia;Eubacteriales;Oscillospiraceae;Oscillibacter;valericigenes_nov_88.501%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000013000000000000069016025992710540136237203038833759612701982110172712707510420445685134386532194961378040514019266236364
SPN781Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Lachnospiraceae_[G-14];bacterium_MOT-185_nov_93.590%000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000009617520212934170251615562410436013597174047786131492263851158872461777106132129242468159
SPN787Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Alloprevotella;sp. HMT308 nov_95.483%0003000063969000620000147290000000000000120000500007300013000000002367200040000940000000001030000000061981800000002960940000000000000220000110000023005963000000004214012000000000000000000000009815000637400116132900165000006000151560000530000430000000000000000000000000000000000000000
SPN795Bacteria;Spirochaetes;Spirochaetia;Spirochaetales;Treponemataceae;Treponema;sp. HMT270 nov_89.837%0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000160000000000000004601511036403600042403216023459039009003301218600800084230
SPN807Archaea;Euryarchaeota;Methanobacteria;Methanobacteriales;Methanobacteriaceae;Methanosphaera;cuniculi_nov_95.652%000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000100000600000040000000000000131842690440505343824721335392102002640111519301201061675800111181199610991320011834
SPN809Bacteria;Firmicutes;Clostridia;Eubacteriales;Oscillospiraceae;Dysosmobacter;welbionis_nov_86.307%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000066892879644550182582879506214153930348733582733510718246545683288824335111646737360
SPN810Bacteria;Firmicutes;Clostridia;Eubacteriales;Oscillospiraceae;Sporobacter;termitidis_nov_86.296%0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000002140000000000000013072900000340041806507421001202562501900036001496000012210
SPN811Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Alloprevotella;sp. HMT473 nov_97.546%1640000000000000000000900000000004100000001600000000000000000000000100000000002100000000000000000000000002000000000000000000000000000000148000000000120001400000000011000008901447000000533825002904600421300089015500139000827097007522000000000000000000000000000000000000000000
SPN813Bacteria;Spirochaetes;Spirochaetia;Spirochaetales;Treponemataceae;Treponema;sp. HMT490 nov_87.247%0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000800000022000000005077611000000000000214407186115480476294042275552748416026741826037429027561741556593478729110451441163639108201954394817646239430816255197549771110256
SPN814Bacteria;Firmicutes;Clostridia;Eubacteriales;Oscillospiraceae;Oscillospiraceae_[G-2];bacterium_MOT-149_nov_91.875%0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000020000000000000005393191110144378506418122268361181386038127379113413362448913711681411082862410969250102120
SPN815Bacteria;Bacteroidetes;Bacteroidetes_[C-1];Bacteroidetes_[O-1];Bacteroidetes_[F-1];Bacteroidetes_[G-3];bacterium HMT436 nov_84.350%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000001600000000000038422333198170177170178177401811325653193069239206889328879250036664591352770281449192
SPN816Bacteria;Firmicutes;Clostridia;Eubacteriales;Oscillospiraceae;Oscillospiraceae_[G-2];bacterium_MOT-149_nov_91.250%0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000200000000000000000000039232811798361823714942179447862102558427778204582945121132151128402057562818645431633354136
SPN817Bacteria;Actinobacteria;Actinomycetia;Corynebacteriales;Corynebacteriaceae;Corynebacterium;durum_nov_91.968%000000000000000000000390011008700000000022000530010400020900000560001226000000015010180000000270000004600010100000000018454000002004200000010014000000000125004500000044700000000180170000000000000046001742410130010200311160006014200039640024082002989500000000000000000000000000000000000000000
SPN818Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;sp. HMT293 nov_89.024%0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000005180000000000009106855416256581393111415610368242103465627028732595088121281962074431281084699210885213457
SPN819Bacteria;Bacteroidetes;Bacteroidetes_[C-1];Bacteroidetes_[O-1];Bacteroidetes_[F-1];Bacteroidetes_[G-3];bacterium HMT365 nov_87.373%0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000050000000000012360116143221302590109206104101235063525152072211156831115215141803970144122318511151227628
SPN820Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Lachnospiraceae_[G-2];bacterium_MOT-167_nov_91.232%000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000001200189230439959123116019076108024501515091807212459001261196011466206010000
SPN821Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Coprococcus;comes_nov_97.275%0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000001436299535514956153637100119256787977334001022034754127751432668172452317417014712013214
SPN822Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Enterocloster;bolteae_nov_91.649%0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000027143101572511132479974265341663315993561204922432661436260121935610355608212864208135175310
SPN823Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;copri_nov_97.755%0000000000000000200000000011000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000011000000000150000000005353000070039000010471182126134812464765923334466867850249858248109720814436826761107271037793134902409156511310221734899109121041136554
SPN824Bacteria;Firmicutes;Clostridia;Eubacteriales;Clostridiaceae;Clostridium;cellulovorans_nov_82.505%000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000140000068172900310000047106141927150000418000
SPN825Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Alloprevotella;sp. HMT473 nov_95.918%609900080154261501313058245356090006001246366530189297103450003891345121665970575538000338127769120411177716022834728695308498345179188700592667236502877511511142808802362852414053514502142711219031037594863513872211626190015193387295944602716681095001338755937137099068113314074333187311040623375311035090694919012328235922162039662109121183853109482215508117195300151533162434438134771202655179219302738596364646305140665019841710265217041036429529911828081233921980002047859212650895032650430347185416187007606739301098136793420340645882611017435940972574804832024122380000000000000000000000000000000000000000
SPN826Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;suis_nov_93.387%7224000974482400016047157194194293620330317525124154124431360153163993123652632931090891117045850619010018232110010321514610723119551214128214561063276878240574360531913200528791030020240069699660176793413831499801315156090404810346240004030600189118015012843101041321510761757730963124011314401231021306143840836102256367799471153975060131579525804285526124211195727544192316257812973083741395350230038281324129133614416131012231382107610000000000000000007000000000000000000000
SPN827Bacteria;Firmicutes;Clostridia;Eubacteriales;Oscillospiraceae;Oscillospiraceae_[G-2];bacterium_MOT-149_nov_88.797%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000667401612200070817718829142945138089462747475951823774426651671791812417307718
SPN828Bacteria;Firmicutes;Clostridia;Eubacteriales;Oscillospiraceae;Oscillibacter;valericigenes_nov_96.050%0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000001100000000000005713318894221513410579694364221012757113150167857944858921195231971098895975712982202911642
SPN829Bacteria;Bacteroidetes;Flavobacteriia;Flavobacteriales;Flavobacteriaceae;Capnocytophaga;ochracea_nov_94.792%0000000035600000000066700000000000000000008300000500001360000020000163000000000186000000000183000001590000000002090000000002680000000008300000000010400700000001090012000000970090000001240011500000000003800000000040000001400000000018006000000000000000000000000000000000000000000
SPN830Bacteria;Firmicutes;Clostridia;Eubacteriales;Peptostreptococcaceae;Peptostreptococcaceae_[G-3];bacterium HMT950 nov_88.727%000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000300000000000000000000000001400013141516710172556014340452110754070106134148129065802241041061527514412267623
SPN831Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;oulorum_nov_89.837%00000000000000000000000000500000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000001300000000040000000048722700000000000041000506900047000720992046035723000015103519011080131802500
SPN832Bacteria;Firmicutes;Clostridia;Eubacteriales;Oscillospiraceae;Oscillospiraceae_[G-2];bacterium_MOT-149_nov_88.706%0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000300000000000000000000000000000010002304250503270034406010533805483000194035512003255001304180
SPN833Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;copri_nov_96.741%0000000000000000000000000000000000004000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000500000000000000000039270000000000000402953260138715905152172436155129132283938332422035252784811391516611652113301358124738
SPN835Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;copri_nov_93.469%0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000039270000000000000102351110423144496049272016238110308400541534103063002500062411615102130400
SPN836Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;saccharolytica_nov_95.092%0000000002100000000003550000000001170000000001230000116000068000040000081000062000050000720000710000200000211000079000014500002600001350000000001940000290010604400007001440188000012500260124000050004607500000000010100000000000000000004000021000000000000000000000000000000000000000000000
SPN837Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Coprococcus;eutactus_nov_95.085%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000008030000000000411340587034718829353310403263921834000018732654849293434103085030225854370
SPN838Bacteria;Fusobacteria;Fusobacteriia;Fusobacteriales;Fusobacteriaceae;Fusobacterium;sp. HMT370 nov_97.629%0000000008890000000002850000000000000000000122000019300000771000050340000451400003695000014000021100004175000029000035530000540500002691000024400001216700004000005208000086550020407231000084020010407420000106600500401720000691003426090460000000001060000058000089000012000036000000000200000000000000000000000000000000000000000
SPN839Bacteria;Proteobacteria;Gammaproteobacteria;Pasteurellales;Pasteurellaceae;Haemophilus;haemolyticus_nov_94.251%4900000005301300000004500100000182290032000003414700000000009220000000000320000000002002002200000000000000000000000000100000000000000000000000000000200000000019000000000140000010047903241820383322411813803137292012908877018374342702025012329000221106000000000000000000000000000000000000000
SPN840Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Tannerellaceae;Tannerella;forsythia_nov_88.613%0000000008000000000020000000000028100000000017200009400001260000300000167000041000000000160000740000102000041900005700002280000220000870000100000139000081000016200005100002030000920000109000032000066000000000160000000000000000000000000000000000000000000000000000000000000000000000
SPN841Bacteria;Firmicutes;Clostridia;Eubacteriales;Oscillospiraceae;Oscillibacter;valericigenes_nov_88.017%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000005300000000000018161874417200184479373101141361566842033102044538167341069238231671092782120523828
SPN842Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;peroris_nov_94.949%037710039870000550001411040000221600010060601000000000396900436001909300002560040070004700298012000010000261000222500000100026000450523500980000099110028000002160150590000209141081040050310020240000711005000007250000007015484006009017030000100000322718000000000000000000000000000000000000000000000
SPN843Bacteria;Firmicutes;Clostridia;Eubacteriales;Oscillospiraceae;Fournierella;massiliensis_nov_91.340%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000356610110352466793255055115331283118622375458148170286252913553222119471223217624509589143
SPN844Bacteria;Bacteroidetes;Bacteroidetes_[C-1];Bacteroidetes_[O-1];Bacteroidetes_[F-1];Bacteroidetes_[G-3];bacterium HMT899 nov_86.939%0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000088771106682150189052003967547047197270538324853771822155019973769631152072012624
SPN846Bacteria;Firmicutes;Clostridia;Eubacteriales;Oscillospiraceae;Sporobacter;termitidis_nov_86.325%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000400000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000052071980186929084336841102256142036282731567021173348725836103389920098080141
SPN847Bacteria;Actinobacteria;Actinomycetia;Corynebacteriales;Corynebacteriaceae;Corynebacterium;massiliense_nov_95.319%00000000000000000000000000000000000000000000000000000000000000000000120000000000000000000000000000005390000000000000000111000000000000000000000000000000000000000000000000007029000008001409214000034847062275739500000000000000000000000000000000000000000000000000
SPN848Bacteria;Firmicutes;Clostridia;Eubacteriales;Clostridiales_[F-1];Clostridiales_[F-1][G-2];bacterium HMT402 nov_84.289%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000901135084154611818012523021431970242204417168320361812370212381031219259516110
SPN849Bacteria;Firmicutes;Clostridia;Eubacteriales;Eubacteriales_[F-1];Eubacteriales_[G-1];bacterium_MOT-144_nov_87.397%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000002400065340315111158210773111481017221513277913381314602852132964371133219555546084312
SPN850Bacteria;Fusobacteria;Fusobacteriia;Fusobacteriales;Leptotrichiaceae;Pseudoleptotrichia;goodfellowii_nov_92.241%622611270139191011014230134141181017670813013199474122855132104431267354336628131735120705742607254697344162158850013830189990830038621900071709321144531022341110202114332412574193627101479691024147403736088300228022610019508555960533519373001001986123507142830225903623193001601441808255062896100426290900515084219008729680070163213210116353870827033650875742724401581901930668031472601012352618977333413041191411232361123201000229291468136730900132330000000000000000000000000000000000000000
SPN851Bacteria;Proteobacteria;Gammaproteobacteria;Pasteurellales;Pasteurellaceae;Aggregatibacter;aphrophilus_nov_96.115%0000000005000007100004650000000001050000000000028000000050064000003800098000000015003800000000000010000000000000000500000023000210060028200024000004000310000001000000000370440000000650000001003101420000420036000106001690220004100138002330310000000000000000000000000000000000000000
SPN852Bacteria;Tenericutes;Mollicutes;Mollicutes_[O-2];Mollicutes_[F-2];Mollicutes_[G-2];bacterium HMT906 nov_82.922%000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000060000111600671836084300130019126161597095200200022
SPN853Bacteria;Actinobacteria;Coriobacteriia;Eggerthellales;Eggerthellaceae;Slackia;equolifaciens_nov_88.362%000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000003000000000000000000000000000352770518342299541153242471013220108900014611441011198781102974448106106218112362918648
SPN854Bacteria;Firmicutes;Clostridia;Eubacteriales;Eubacteriales_[F-1];Eubacteriales_[G-1];bacterium_MOT-144_nov_89.278%000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000400000000000000000000000007032271562861940140194813012216733074924120421171976419910665319179112240933824
SPN857Bacteria;Synergistetes;Synergistia;Synergistales;Synergistaceae;Fretibacterium;sp. HMT362 nov_97.817%0000000006100000000012300000000057000000000150000000004600000000013100004600000000000000720000000710429000034000055000000002660210000000000117000000011011700003600001540000300010500000000034088000000000470000000000000000000000000000000000000000000000000000000000000000000000
SPN858Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;oulorum_nov_89.837%0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000003000000000011261100000000000095226558672601031315451142591501156813818308301103153237324660586218122283933127012237
SPN859Bacteria;Actinobacteria;Actinomycetia;Actinomycetales;Actinomycetaceae;Schaalia;sp. HMT178 nov_94.908%000000017200000000027100000000011600000000014700000000024100000000059000000003280000000005000000000025500000000012200000000035400000000080000000001720000000001390000000004310000000002520000000000000000000000000000000000000000000000000000000000000000000000000000000000
SPN860Bacteria;Firmicutes;Clostridia;Eubacteriales;Oscillospiraceae;Oscillibacter;valericigenes_nov_86.392%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000060000000312070591274301773171064103101203953294029702738876311555348161611645459120482682103104
SPN861Bacteria;Firmicutes;Clostridia;Eubacteriales;Oscillospiraceae;Oscillospiraceae_[G-2];bacterium_MOT-149_nov_90.269%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000776491398469196248213207373717553651522938358052100487646245935432192231585714114130121
SPN862Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;gordonii_nov_94.036%26002824128290000810211532846159623436017800520152785341503682007650226306867160003923601757286101185008944734170115532114021228269073172903472355122720263900447115028713593722153694001032146016402476242816000466046221893985651395603581840587289991000012552864262933473944500952545612791741189329170068041668792233000581204683493397944000015174024448981173900002252957144655409421690023661632212253315206179102166860180221667992710000310000000000000000000000000000000000000000
SPN863Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Anaerobutyricum;hallii_nov_96.842%00000000000000000000000000000000000040000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000190070000000000043117500072851194316982012395911502215513122722455987151028035271700773065355422
SPN864Bacteria;Bacteroidetes;Bacteroidetes_[C-1];Bacteroidetes_[O-1];Bacteroidetes_[F-1];Bacteroidetes_[G-3];bacterium HMT280 nov_89.485%0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000006000000000001100000000000001320001900113030209327838203192301572088403265117907746013210471214108861023
SPN865Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Oribacterium;sinus_nov_96.429%00000000000000000000000000000000000000000000000000110000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000008203470504850020618057309301920024521018705702180029000000000000000000000000000000000000000000
SPN866Bacteria;Firmicutes;Negativicutes;Veillonellales;Veillonellaceae;Megasphaera;elsdenii_nov_93.898%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000315539368432501279303178275243265365674434593077292180001173920951855162001360
SPN868Bacteria;Firmicutes;Clostridia;Eubacteriales;Eubacteriaceae;Eubacterium;xylanophilum_nov_90.707%0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000006000000000000000000000000001432396905392291060102513860118286040210729727291332604107150022971844301603719
SPN869Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Prevotellaceae;Prevotella;copri_nov_95.927%000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000013000000000000282511936245266571482929755090432343118595515020844332721560281211522316691521018117
SPN870Bacteria;Firmicutes;Erysipelotrichia;Erysipelotrichales;Erysipelotrichaceae;Solobacterium;moorei_nov_91.546%000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000200000000000000000000000000000000000000000000000000000000000200000000000000001300000000000000553037338772512578412657235647351610210725172276143706858518217855431741288767361049727
SPN871Bacteria;Actinobacteria;Coriobacteriia;Coriobacteriales;Atopobiaceae;Olsenella;uli_nov_94.872%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000010000000000000009133412313325203294227333118171826386161741288066421312720072306164144133140611523925
SPN872Bacteria;Firmicutes;Clostridia;Eubacteriales;Eubacteriales_[F-1];Eubacteriales_[G-1];bacterium_MOT-144_nov_86.680%000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000004531271910134281204467949013518518916226014815421431909432114411872010251016081622114
SPN873Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Muribaculaceae;Muribaculaceae_[G-1];bacterium_MOT-129_nov_80.894%000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000010000000000000000056001731900000141139611750076000447458791182859600021109122582920
SPN874Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;oralis_subsp._tigurinus_clade_071_nov_96.162%654446029691012163501066127690411811361062304662654032011176146260022125056331201318030281042022039063507427301757407726112808501166057211320944015350620239017743020600001006287027900033205981061053001270020634652039800618017979070300502113952384113543771970385228181292038882124681020112309439858017321421273730607327459790144218984348434058511558194157915908801451374459527785529066011070681821557280203030012128110912550928841120470000000000000000000000000000000000000000
SPN875Bacteria;Firmicutes;Clostridia;Eubacteriales;Christensenellaceae;Christensenella;timonensis_nov_82.992%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000111602335009510018037140431545180471970800101582711930183005364562742
SPN876Bacteria;Firmicutes;Clostridia;Eubacteriales;Peptostreptococcaceae;Terrisporobacter;mayombei_nov_97.778%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000248000000000000043135314820910971177018130008703300037085734688212117006463046169925135600
SPN877Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Muribaculaceae;Muribaculaceae_[G-2];bacterium_MOT-104_nov_89.157%000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000900000000000004210570161625019018120180619920200930380534793783094001422011192020521104
SPN879Bacteria;Firmicutes;Clostridia;Eubacteriales;Oscillospiraceae;Agathobaculum;desmolans_nov_86.947%0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000300000000000000000020000000000000003298831712114577353737482669536828372565496912392105134532151067272451210125449411048
SPN88Bacteria;Bacteroidetes;Bacteroidetes_[C-1];Bacteroidetes_[O-1];Bacteroidetes_[F-1];Bacteroidetes_[G-3];bacterium HMT365 nov_87.935%000000000000000000000000000000000000000000000000000000000000000000000000000300000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000003201500000000000086831942011403514723532291467114511973145152205155032851183547497310025115292003122796381141161468661
SPN880Bacteria;Firmicutes;Clostridia;Eubacteriales;Oscillospiraceae;Caproiciproducens;galactitolivorans_nov_87.110%0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000002100000000000000061000107075000100000860550000000189496445500150314005400150
SPN881Bacteria;Actinobacteria;Actinomycetia;Micrococcales;Micrococcaceae;Rothia;dentocariosa_nov_96.603%0000000000400000000000000000000000007000480000000004600000000000000060000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000007131902700210350118003509740280360000021401710590000002370900000000000000000000000000000000000000000
SPN882Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Coprococcus;eutactus_nov_95.923%0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000001500000000000007313302530602343700101528618513701002935114514668680225321122721701512210
SPN883Bacteria;Proteobacteria;Alphaproteobacteria;Hyphomicrobiales;Rhizobiaceae;Agrobacterium;fabacearum_nov_97.902%000000000000000000000000070000000000070200100405000000000060000000000000350000000023230000000000000000003100000000200000000000000000000000000000000000000000002000000000000000000000000000000000000000000000000000000000000000000000000000000000000000
SPN884Bacteria;Fusobacteria;Fusobacteriia;Fusobacteriales;Leptotrichiaceae;Pseudoleptotrichia;sp. HMT219 nov_94.600%025009000134005700000043811800000000012800002100006250000260009002100000010770000150000702500550000110200012700099000001300054000001200000000096000000000900030000000000700000000000000001300006000000000340000900000000000000000000000000000000000000000000000000000000000000000
SPN885Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Bacteroidales_[F-2];Bacteroidales_[G-2];bacterium HMT274 nov_82.591%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000230320105000002104374423161278081108802119533447141703538631672333174899
SPN886Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Lachnospiraceae_[G-3];bacterium HMT100 nov_96.868%010165150202001334628113701034589035600178050282702426120258001481034408991820386002974811216552791310170019421684084610322544010010191323382120301681261972058142825886104965348612900170126493093211005872165801089888834086442123148121704175161192981256211051140043421364234577087661382410278335155145317751766171289937315262728412454335362138801211673331901179220766249993706781271220105427160603771455533162300151157011950046753921361573001201530025851925523144490852160351981916825155108140775511131490000000000000000000000000000000000000000
SPN887Bacteria;Actinobacteria;Coriobacteriia;Coriobacteriales;Atopobiaceae;Lancefieldella;sp. HMT199 nov_97.639%000400000000000000000001541500000000430000000007000000000000000000000000000002255300000000375000000000000000000020000000001036000000000000000000000000000000000000000397000000400329000004000000000600900000000030000000000000000000000000000000000000000000000
SPN888Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;periodonticum_nov_97.967%0300000000000000000000001100000000000000000000050000000000000000000120000000006000000000600000000000000000000000000000000000000000000000000000000000000000000000003313700277802920297974042671911961440236736116251045801501219006470682181410180000000000000000000000000000000000000000
SPN890Bacteria;Firmicutes;Erysipelotrichia;Erysipelotrichales;Erysipelotrichaceae;Solobacterium;moorei_nov_88.367%0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000001200000000000003441455686547046413140495441856028113983533358310694634987369452609638686376405329
SPN891Bacteria;Firmicutes;Clostridia;Eubacteriales;Oscillospiraceae;Oscillospiraceae_[G-2];bacterium_MOT-149_nov_93.125%0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000170000000000001514190502130384116588515630204961151189261233151041043930427204103884250340
SPN892Bacteria;Tenericutes;Mollicutes;Mollicutes_[O-2];Mollicutes_[F-2];Mollicutes_[G-2];bacterium_MOT-188_nov_83.939%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000093712103455030607390107561982140244288752432007343117315595402982019777
SPN893Bacteria;Proteobacteria;Deltaproteobacteria;Desulfovibrionales;Desulfovibrionaceae;Desulfovibrio;intestinalis_nov_96.552%0000000000000000000000050100000000051000000000000000000000000000000003000000012000028200000000000000000000000000000000000013400000000000000000000000000000000000000052000000000700000000040000000000600000000000000000000000000000000000000000000000000000000
SPN894Bacteria;Bacteroidetes;Bacteroidetes_[C-1];Bacteroidetes_[O-1];Bacteroidetes_[F-1];Bacteroidetes_[G-3];bacterium HMT365 nov_87.526%000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000008005343741305728334447935196161871725056191510322351301212719276230091159
SPN895Bacteria;Firmicutes;Negativicutes;Veillonellales;Veillonellaceae;Dialister;succinatiphilus_nov_93.177%0000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000006000000000000046266227318313380237172901291912465454437160235944030649075271968241846223424
SPN898Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Lachnospiraceae_[G-2];bacterium HMT088 nov_93.933%8095406854317019033142061711236308037533030318881358177370523701441944589361539174411027190165389595230391302257111010453241051276132721804281242290317550000801003900006075005702415143356311201002341013194712678001583246237015724582602591100957531754001201279312615811203502253922021157114892201314822613462522118681228511619345361407198227109916378692321527792321441199937084014369093471383319958261260757408584785134220231821412560000000000000000000000000000000000000000
SPN910Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;gordonii_nov_93.638%14800320735470014800120185621700107001406521139580015900712417197500535430204598119814107246018665226924601314217062309713727168063014190345184604318265198852121327046698204200172072851975371601336250677882289702955001922316118893313951156702278126604211892399887436101612870171441542016719054225420729339301524802673121456239422733961062896996462066841793071240156800332309293203607211432331123436303231630831072977933202212872855804363113772851192728580202104900000000000000000000000000000000000000000
SPN923Bacteria;Proteobacteria;Gammaproteobacteria;Pasteurellales;Pasteurellaceae;Aggregatibacter;sp. HMT898 nov_94.694%10429532414238213573300150941895283730619113522300020812682404589026916800331934664779374306212038810618706534161526016160013201943392658188089472500457087176600101652410013107726014600503000006291093353568425103842075611251817213878126002304525691305242411500378267949738263920070017084255673672740005302091926140605201540000159352204009680008001312245878552000111411613311741271020162508199170885237037701161671303592110000128642774800015130000000000000000000000000000000000000000
SPN935Bacteria;Proteobacteria;Gammaproteobacteria;Pasteurellales;Pasteurellaceae;Aggregatibacter;aphrophilus_nov_95.697%001541182062225041373400529430913180248799005832840000018090029580500000108501554768176019139083012460230274025907141901363074012273523370799130024830268112586004960004130836278821443600013185301004283824200006320570904519292023000013240108120169900000377066835492410117431443101416019982502122811117930310202900497394186226494550201701011241561057262812205056700384900000912201083520000074001000101000011500689820001126580000000000000000000000000000000000000000
SPN944Bacteria;Actinobacteria;Actinomycetia;Actinomycetales;Actinomycetaceae;Actinomyces;viscosus_nov_97.546%0007027000012700015370000068500019320000235100012470000246600013452021001142071019034000511133000180093000000142905600585000124343000097502502844001501237000383090014313100429902080013320330476300410182057712700016017600042000005260001600000190000202000021000121000000000300000000921000000000000000000000000000000000000000000000
SPN946Bacteria;Firmicutes;Bacilli;Lactobacillales;Aerococcaceae;Abiotrophia;defectiva_nov_95.609%22625725981258111540139945512153723771820723285677716270239186210181850680127351067898163829176930751502268106291342486443321617375284477208313917011481626588316147664441065622436585171689864103661117233723623984965333333389144845242144289706602911430347920998000172438136648046301230763332698392134644597147264619895966737721088771656324564516686410218989236837178598058113158848718830926307142272330297176562129260917159996555918478964673621342012314153004242464774239374197618475853865297602598143133891147167238204983812792810492644285183255154571932127916691966115495881042277479784395157414051516336257557277314230000000000000000000000000000000000000000
SPN947Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;gordonii_nov_91.849%297800083142742692305660001311724746331018510024670131853126157541701715001261650320633154112590869000106013751260582000116271412700147400681371932397916821350260500147881360295182439100000444521842490012000015015020001880011035261733224185316048900663805067216766812340140001542231481700580002130215796012300277119970222919315505590063929251738264550510006539150535203800583109784358000060432237570967001441082363618400000000000000000000000000000000000000000
SPN957Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Porphyromonadaceae;Porphyromonas;pasteri_nov_97.947%004929154003533005020053223200287700844002292201186003605026293550062900111000668326001940319800159232001430148218040000600050730251685600178000005214960000022002915300286003957023587100249008470002261600032940016004760006903820081673001290029630100008500127710126300016403270131030331461280113119094119500322016456060513313400462630602523791139127571600000000000000000000000000000000000000000
SPN969Bacteria;Proteobacteria;Gammaproteobacteria;Pasteurellales;Pasteurellaceae;Haemophilus;parahaemolyticus_nov_97.342%290280706022589010160207469093271012053222669344702104424546013216582047183034239180100321786017143226039523148741341021912159120917618107700610938616919025130005712175311011274018002827300248132701231451918243113503001073625541250584600029170423394617908538026112038109465073014285965942591900465461861061101372360296439210093761863735213132254931701283274668141192009153064711013120101530327892771764187610212619504718011732100924348717150000000000000000000000000000000000000000
SPN98Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Tannerellaceae;Parabacteroides;goldsteinii_nov_83.333%000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000002000003121047826491302087048612001465724032146913434401014923901051779243700506210371015
SPN981Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;sinensis_nov_91.683%277900069023721024346500013215739857640158400387622277229441250729615740019014702824287730336710796000127011057117068000995201251412251293001079916563483156223954623890018716133526487367072000003004415213512400000191125600591460016435256962835173581174160012036345722071602196712118001414249711724480033183318284946860048696820208216932746522009798766158824710884700314849159497524340014531044641105531101029349001295076002071311115357131980000000000000000000000000000000000000000
SPN993Bacteria;Firmicutes;Bacilli;Lactobacillales;Carnobacteriaceae;Granulicatella;elegans_nov_96.970%27134220918013328110122125798401210019216215410412304611182080112174626792687112831517161700161058372211172041232318386100256942875867496723401437442410255033129680000528132320713950260017356012140075171619683777905131012912235162240061011400261803693410015181718800003205473217925351488452133536139415889671266476311477522862041159597993311871399285207289720452022271262220103498143312189952406103712001046248014822797651248516541710288010211397161213750000000000000000000000000000000000000000
SPP11Bacteria;Firmicutes;Bacilli;Bacillales;Staphylococcaceae;Staphylococcus;multispecies_spp11_2020000000000000000000000000000000000000000000000000000000000000000008400000000200000000000030000000100000020011000000000000000000000000000000000000000200000000000211500065512608160001718120720510008351236611327258019200000300000000000000000000000000000000000000000
SPP18Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;multispecies_spp18_21100056514131933600532003550711127106004820001142715395710022700021143171928300700011881505198003990041570170148004009369122600340001800351000001619420000000060260022000234901450000001795062500017200804022110002780072116164500000061908691790003770034684938500086005642734486571685013720200255213213551850043004156107109100121177001822014361490000000000000000000000000000000000000000000
SPP20Bacteria;Firmicutes;Bacilli;Lactobacillales;Streptococcaceae;Streptococcus;multispecies_spp20_20700000000000000000000000000000800000000181000000000132160000000000000008000170040000000002600000000000000000300000000000000000000000000060000130000000000000000000700000043400000000491675503760023200202205512024100483003019447050700206000000000000000000000000000000000000000000
SPP21Bacteria;Synergistetes;Synergistia;Synergistales;Synergistaceae;Fretibacterium;multispecies_spp21_20000000032300000000017100000000000000000000000003500388000000000309500000433001220000005700450000000004708100000270024815000000000189700000013008500000014002039300000000259168000005600906000000400099700000000000000009002000000000000000000000000000000000000000000000000000000000000000
SPP32Bacteria;Firmicutes;Bacilli;Bacillales;Staphylococcaceae;Staphylococcus;multispecies_spp32_20300000000000000001600000001150300014000000151700000000260100000000000130021000000504000001227100000800000000000001900000000060014000018000000000000000000000000000002600000000000411204802964560603435308000533536012992180702101421764191538907700257680000000000000000000000000000000080000000000
SPP50Bacteria;Bacteroidetes;Flavobacteriia;Flavobacteriales;Flavobacteriaceae;Capnocytophaga;multispecies_spp50_2000000014700000020000000000210019000000840032000000000165000000000000000054600600000000524000000000000000000000000000003160025000000394003600000000000000000000000000104002100000019300340000001500000000015000000000300000000000000000000000000000000000000000000000000000000
SPP6Bacteria;Fusobacteria;Fusobacteriia;Fusobacteriales;Fusobacteriaceae;Fusobacterium;multispecies_spp6_21161111426179403163589986752119280919722408329523130484512029634172956104409031318852179681210370767820487614453749041512307184336322743096198328076328161507930582877305135150581443107466927323941521227311893276722522499786565422117016230841457287240944307824830197946782751182633124032591170436277725943082277064012247114310231652758358047591269522277262922213191249146865447426094184668249632876584416411267276515924733684225119763425016081147603578240739108801009594353036503511621336183446172421801967291823950045159008178403038995163471792150896033722710617661398013001007397544252335001170835350000000000000000000000000000000000000000
SPPN1Bacteria;Firmicutes;Clostridia;Eubacteriales;Peptostreptococcaceae;multigenus;multispecies_sppn1_2_nov_96.800%010090889070290802055001906028900235418290201100155202664020543830174001320030710306127034001189116052160171547701346001955214626010990409013700017719881647093647810261001907668223406362791010100255113819160164300446200218623652172021510020800013141186197101141401140020907482305015487055001053139623190101641903881007514292194808503570151400108816455335082785005350058015562155095585304100790148011112043001343384603846013500177258103058403900156481610204706600000000000000000000000000000000000000000
SPPN11Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Tannerellaceae;Tannerella;multispecies_sppn11_2_nov_97.934%00000125900025100000367170065000022500000262000460200081110000101000840000019700063000940800000004680023001830000000003430000000002400001260000026100043300000430000000000000000000013500000000017400027800000175180000614001452900007270003400000000000000000000000000000000000000000000000000000000000
SPPN23Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Porphyromonadaceae;Porphyromonas;multispecies_sppn23_2_nov_96.920%000000000137000000000271000000000278000000000115000000000254000000000810000000003000000000122000000000317000000000116000000000490000000004090000000005590000000001302000000000353000000000322000000000400000000003800000000018000000000340000000000000000000000000000000000000000
SPPN31Bacteria;Firmicutes;Negativicutes;Veillonellales;Veillonellaceae;Veillonella;multispecies_sppn31_4_nov_95.088%00000000000000000000200000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000001290011301760169234000018480000001226161310281700380000181000012520000000000000000000000000000000000000
SPPN32Bacteria;Bacteroidetes;Bacteroidetes_[C-1];Bacteroidetes_[O-1];Bacteroidetes_[F-1];Bacteroidetes_[G-3];multispecies_sppn32_2_nov_86.939%00000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000001900000000000007510638642525514712476851632527011728201220433691802613123145120181166504013512239262340
SPPN34Bacteria;Bacteroidetes;Bacteroidia;Bacteroidales;Porphyromonadaceae;Porphyromonas;multispecies_sppn34_2_nov_96.115%936046375654630297161724371214319289814099346314531058190957630725468125606864910160534357276147618561637134999446723347102227780384800369246250565162717424310101373473861300994440270508110142391612499988491187348933101230340057307521839218553341049605910658344298254435043907776970200116199313039213440715191045152409114406903540673145393199027006854671435207118702901621701276139078002401556581054192361791186037403718027205470132103192631026789014743614222024102181000811812703415100240740000000000000000000000000000000000000000
SPPN42Bacteria;Firmicutes;Negativicutes;Selenomonadales;Selenomonadaceae;Selenomonas;multispecies_sppn42_2_nov_97.030%019420100000166920232610000027514306712900000147607514200000122003901451562000080460114040600057319085291757980000641007452647075900021912049519929618500041375010515122653000332860869132242880007840011351245095000405905851624405560002982001461089574000384740153932133016630019851416401419199514557004882755302700000006507123010000220033927290000000334102600002400000000000000000000000000000000000000000
SPPN48Bacteria;Firmicutes;Clostridia;Eubacteriales;Peptostreptococcaceae;multigenus;multispecies_sppn48_2_nov_92.083%0000000000000000000000000040000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000006000003050000000001380000110000130025679313353185254493316611401041811522613535553615794195581917743884606685931090528864331801100055578654956608346
SPPN5Bacteria;Spirochaetes;Spirochaetia;Spirochaetales;Treponemataceae;Treponema;multispecies_sppn5_3_nov_97.566%00000000056000000000000000000000000000001010000000004710000000008320000000000000000000150400000000092100000000085300000000074600000000089900000000041400000000028700000000025600000000071900000000000000000007000000000000000000000000000000000000000000000000000000000000
SPPN57Bacteria;Bacteroidetes;Flavobacteriia;Flavobacteriales;Flavobacteriaceae;Capnocytophaga;multispecies_sppn57_2_nov_96.855%00000000031900000000074000000000018380000000002022000000000289000000000407000000000254200000000033300000000011300000000043500000000061500000000025600000000034000000000164000000000306000000000134000000000254000000000200000000009000000000250000000000000000000000000000000000000000
SPPN59Bacteria;Firmicutes;Clostridia;Eubacteriales;Lachnospiraceae;Faecalicatena;multispecies_sppn59_2_nov_96.868%000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000001110000000000000014418769164210295178159166114167234532092551261301043761141042415935675164501541726744632585318750765328526420318774
SPPN69Bacteria;Proteobacteria;Gammaproteobacteria;Pasteurellales;Pasteurellaceae;Aggregatibacter;multispecies_sppn69_2_nov_97.536%6471475401178233512990532742760014461996767801871523727965006933439713314371681257944310838739695113355912014221074165588397232712410435743215448126733014362181086910563476559497893862236162290261166394245319814358400406839088218180210091314914502159601384753935913830986512403676647218752268119129438157280259281139417013547250126837691611003278792475109564149742199110242554336352012423098041335163391570369787141332554023266015835816432333732216429449313255316474418230714391803041526429119504861181442501700000000000000000000000000000000000000000
SPPN8Bacteria;Firmicutes;Erysipelotrichia;Erysipelotrichales;Erysipelotrichaceae;Erysipelothrix;multispecies_sppn8_2_nov_84.739%000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000000730000900030000001000000000000600701122541492212541389146246831172086921210584501313749944212330193108173160367249120140138136343180107473257103
 
 
Download OTU Tables at Different Taxonomy Levels
PhylumCount*: Relative**: CLR***:
ClassCount*: Relative**: CLR***:
OrderCount*: Relative**: CLR***:
FamilyCount*: Relative**: CLR***:
GenusCount*: Relative**: CLR***:
SpeciesCount*: Relative**: CLR***:
* Read count
** Relative abundance (count/total sample count)
*** Centered log ratio transformed abundance
;
 
The species listed in the table has full taxonomy and a dynamically assigned species ID specific to this report. When some reads match with the reference sequences of more than one species equally (i.e., same percent identiy and alignmnet coverage), they can't be assigned to a particular species. Instead, they are assigned to multiple species with the species notaton "s__multispecies_spp2_2". In this notation, spp2 is the dynamic ID assigned to these reads that hit multiple sequences and the "_2" at the end of the notation means there are two species in the spp2.

You can look up which species are included in the multi-species assignment, in this table below:
 
 
 
 
Another type of notation is "s__multispecies_sppn2_2", in which the "n" in the sppn2 means it's a potential novel species because all the reads in this species have < 98% idenity to any of the reference sequences. They were grouped together based on de novo OTU clustering at 98% identity cutoff. And then a representative sequence was chosed to BLASTN search against the reference database to find the closest match (but will still be < 98%). This representative sequence also matched equally to more than one species, hence the "spp" was given in the label.
 
 

Taxonomy Bar Plots for All Samples

 
 

Taxonomy Bar Plots for Individual Comparison Groups

 
 
Comparison No.Comparison NameFamiliesGeneraSpecies
Comparison 1Saliva_D0_Gp1 vs Saliva_D0_Gp2PDFSVGPDFSVGPDFSVG
Comparison 2Saliva_D56_Gp1 vs Saliva_D56_Gp2PDFSVGPDFSVGPDFSVG
Comparison 3Saliva_D70_Gp1 vs Saliva_D70_Gp2PDFSVGPDFSVGPDFSVG
Comparison 4Saliva_D91_Gp1 vs Saliva_D91_Gp2PDFSVGPDFSVGPDFSVG
Comparison 5Saliva_D0_Gp1 vs Saliva_D56_Gp1 vs Saliva_D70_Gp1 vs Saliva_D91_Gp1PDFSVGPDFSVGPDFSVG
Comparison 6Saliva_D0_Gp2 vs Saliva_D56_Gp2 vs Saliva_D70_Gp2 vs Saliva_D91_Gp2PDFSVGPDFSVGPDFSVG
Comparison 7Feces_D0_Gp1 vs Feces_D0_Gp2PDFSVGPDFSVGPDFSVG
Comparison 8Feces_D56_Gp1 vs Feces_D56_Gp2PDFSVGPDFSVGPDFSVG
Comparison 9Feces_D70_Gp1 vs Feces_D70_Gp2PDFSVGPDFSVGPDFSVG
Comparison 10Feces_D91_Gp1 vs Feces_D91_Gp2PDFSVGPDFSVGPDFSVG
Comparison 11Feces_D0_Gp1 vs Feces_D56_Gp1 vs Feces_D70_Gp1 vs Feces_D91_Gp1PDFSVGPDFSVGPDFSVG
Comparison 12Feces_D0_Gp2 vs Feces_D56_Gp2 vs Feces_D70_Gp2 vs Feces_D91_Gp2PDFSVGPDFSVGPDFSVG
Comparison 13Plaque_D0_Gp1_Site12 vs Plaque_D0_Gp2_Site12PDFSVGPDFSVGPDFSVG
Comparison 14Plaque_D56_Gp1_Site12 vs Plaque_D56_Gp2_Site12PDFSVGPDFSVGPDFSVG
Comparison 15Plaque_D70_Gp1_Site12 vs Plaque_D70_Gp2_Site12PDFSVGPDFSVGPDFSVG
Comparison 16Plaque_D91_Gp1_Site12 vs Plaque_D91_Gp2_Site12PDFSVGPDFSVGPDFSVG
Comparison 17Plaque_D0_Gp1_Site12 vs Plaque_D56_Gp1_Site12 vs Plaque_D70_Gp1_Site12 vs Plaque_D91_Gp1_Site12PDFSVGPDFSVGPDFSVG
Comparison 18Plaque_D0_Gp2_Site12 vs Plaque_D56_Gp2_Site12 vs Plaque_D70_Gp2_Site12 vs Plaque_D91_Gp2_Site12PDFSVGPDFSVGPDFSVG
Comparison 19Plaque_D0_Gp1_Site34 vs Plaque_D0_Gp2_Site34PDFSVGPDFSVGPDFSVG
Comparison 20Plaque_D56_Gp1_Site34 vs Plaque_D56_Gp2_Site34PDFSVGPDFSVGPDFSVG
Comparison 21Plaque_D70_Gp1_Site34 vs Plaque_D70_Gp2_Site34PDFSVGPDFSVGPDFSVG
Comparison 22Plaque_D91_Gp1_Site34 vs Plaque_D91_Gp2_Site34PDFSVGPDFSVGPDFSVG
Comparison 23Plaque_D0_Gp1_Site34 vs Plaque_D56_Gp1_Site34 vs Plaque_D70_Gp1_Site34 vs Plaque_D91_Gp1_Site34PDFSVGPDFSVGPDFSVG
Comparison 24Plaque_D0_Gp2_Site34 vs Plaque_D56_Gp2_Site34 vs Plaque_D70_Gp2_Site34 vs Plaque_D91_Gp2_Site34PDFSVGPDFSVGPDFSVG
 
 

VIII. Analysis - Alpha Diversity

 

In ecology, alpha diversity (α-diversity) is the mean species diversity in sites or habitats at a local scale. The term was introduced by R. H. Whittaker[5][6] together with the terms beta diversity (β-diversity) and gamma diversity (γ-diversity). Whittaker's idea was that the total species diversity in a landscape (gamma diversity) is determined by two different things, the mean species diversity in sites or habitats at a more local scale (alpha diversity) and the differentiation among those habitats (beta diversity).

 

References:

  1. Whittaker, R. H. (1960) Vegetation of the Siskiyou Mountains, Oregon and California. Ecological Monographs, 30, 279–338. doi:10.2307/1943563
  2. Whittaker, R. H. (1972). Evolution and Measurement of Species Diversity. Taxon, 21, 213-251. doi:10.2307/1218190

 

Alpha Diversity Analysis by Rarefaction

Diversity measures are affected by the sampling depth. Rarefaction is a technique to assess species richness from the results of sampling. Rarefaction allows the calculation of species richness for a given number of individual samples, based on the construction of so-called rarefaction curves. This curve is a plot of the number of species as a function of the number of samples. Rarefaction curves generally grow rapidly at first, as the most common species are found, but the curves plateau as only the rarest species remain to be sampled [7].


References:

  1. Willis AD. Rarefaction, Alpha Diversity, and Statistics. Front Microbiol. 2019 Oct 23;10:2407. doi: 10.3389/fmicb.2019.02407. PMID: 31708888; PMCID: PMC6819366.

 
 
 

Boxplot of Alpha-diversity Indices

The two main factors taken into account when measuring diversity are richness and evenness. Richness is a measure of the number of different kinds of organisms present in a particular area. Evenness compares the similarity of the population size of each of the species present. There are many different ways to measure the richness and evenness. These measurements are called "estimators" or "indices". Below is a diversity of 3 commonly used indices showing the values for all the samples (dots) and in groups (boxes).

Printed on each graph is the statistical significance p values of the difference between the groups. The significance is calculated using either Kruskal-Wallis test or the Wilcoxon rank sum test, both are non-parametric methods (since microbiome read count data are considered non-normally distributed) for testing whether samples originate from the same distribution (i.e., no difference between groups). The Kruskal-Wallis test is used to compare three or more independent groups to determine if there are statistically significant differences between their medians. The Wilcoxon Rank Sum test, also known as the Mann-Whitney U test, is used to compare two independent groups to determine if there is a significant difference between their distributions.
The p-value is shown on the top of each graph. A p-value < 0.05 is considered statistically significant between/among the test groups.

 
Alpha Diversity Box Plots for All Groups
 
 
 
 
 
 
 
Alpha Diversity Box Plots for Individual Comparisons at Species level
 
Comparison 1Saliva_D0_Gp1 vs Saliva_D0_Gp2View in PDFView in SVG
Comparison 2Saliva_D56_Gp1 vs Saliva_D56_Gp2View in PDFView in SVG
Comparison 3Saliva_D70_Gp1 vs Saliva_D70_Gp2View in PDFView in SVG
Comparison 4Saliva_D91_Gp1 vs Saliva_D91_Gp2View in PDFView in SVG
Comparison 5Saliva_D0_Gp1 vs Saliva_D56_Gp1 vs Saliva_D70_Gp1 vs Saliva_D91_Gp1View in PDFView in SVG
Comparison 6Saliva_D0_Gp2 vs Saliva_D56_Gp2 vs Saliva_D70_Gp2 vs Saliva_D91_Gp2View in PDFView in SVG
Comparison 7Feces_D0_Gp1 vs Feces_D0_Gp2View in PDFView in SVG
Comparison 8Feces_D56_Gp1 vs Feces_D56_Gp2View in PDFView in SVG
Comparison 9Feces_D70_Gp1 vs Feces_D70_Gp2View in PDFView in SVG
Comparison 10Feces_D91_Gp1 vs Feces_D91_Gp2View in PDFView in SVG
Comparison 11Feces_D0_Gp1 vs Feces_D56_Gp1 vs Feces_D70_Gp1 vs Feces_D91_Gp1View in PDFView in SVG
Comparison 12Feces_D0_Gp2 vs Feces_D56_Gp2 vs Feces_D70_Gp2 vs Feces_D91_Gp2View in PDFView in SVG
Comparison 13Plaque_D0_Gp1_Site12 vs Plaque_D0_Gp2_Site12View in PDFView in SVG
Comparison 14Plaque_D56_Gp1_Site12 vs Plaque_D56_Gp2_Site12View in PDFView in SVG
Comparison 15Plaque_D70_Gp1_Site12 vs Plaque_D70_Gp2_Site12View in PDFView in SVG
Comparison 16Plaque_D91_Gp1_Site12 vs Plaque_D91_Gp2_Site12View in PDFView in SVG
Comparison 17Plaque_D0_Gp1_Site12 vs Plaque_D56_Gp1_Site12 vs Plaque_D70_Gp1_Site12 vs Plaque_D91_Gp1_Site12View in PDFView in SVG
Comparison 18Plaque_D0_Gp2_Site12 vs Plaque_D56_Gp2_Site12 vs Plaque_D70_Gp2_Site12 vs Plaque_D91_Gp2_Site12View in PDFView in SVG
Comparison 19Plaque_D0_Gp1_Site34 vs Plaque_D0_Gp2_Site34View in PDFView in SVG
Comparison 20Plaque_D56_Gp1_Site34 vs Plaque_D56_Gp2_Site34View in PDFView in SVG
Comparison 21Plaque_D70_Gp1_Site34 vs Plaque_D70_Gp2_Site34View in PDFView in SVG
Comparison 22Plaque_D91_Gp1_Site34 vs Plaque_D91_Gp2_Site34View in PDFView in SVG
Comparison 23Plaque_D0_Gp1_Site34 vs Plaque_D56_Gp1_Site34 vs Plaque_D70_Gp1_Site34 vs Plaque_D91_Gp1_Site34View in PDFView in SVG
Comparison 24Plaque_D0_Gp2_Site34 vs Plaque_D56_Gp2_Site34 vs Plaque_D70_Gp2_Site34 vs Plaque_D91_Gp2_Site34View in PDFView in SVG
 
The above comparisons are at the species-level. Comparisons of other taxonomy levels, from phylum to genus, are also available:
 
 
 
 

Group Significance Evaluation of Alpha-diversity Indices with QIIME2

The above comparisons and significance tests were done under the R environment. For compasison (also because this was included in the pipeline early on) we also use the Kruskal Wallis H test provided the "alpha-group-significance" fucntion in the QIIME 2 "diversity" package. As mentioned above, Kruskal Wallis test is the non-parametric alternative to the One Way ANOVA. Non-parametric means that the test doesn’t assume your data comes from a particular distribution. The H test is used when the assumptions for ANOVA aren’t met (assumption of normality). It is sometimes called the one-way ANOVA on ranks, as the ranks of the data values are used in the test rather than the actual data points. The H test determines whether the medians of two or more groups are different.

Below are the Kruskal Wallis H test results for each comparison based on three different alpha diversity measures: 1) Observed species (features), 2) Shannon index, and 3) Simpson index.

 
 
Comparison 1.Saliva_D0_Gp1 vs Saliva_D0_Gp2Observed FeaturesShannon IndexSimpson Index
Comparison 2.Saliva_D56_Gp1 vs Saliva_D56_Gp2Observed FeaturesShannon IndexSimpson Index
Comparison 3.Saliva_D70_Gp1 vs Saliva_D70_Gp2Observed FeaturesShannon IndexSimpson Index
Comparison 4.Saliva_D91_Gp1 vs Saliva_D91_Gp2Observed FeaturesShannon IndexSimpson Index
Comparison 5.Saliva_D0_Gp1 vs Saliva_D56_Gp1 vs Saliva_D70_Gp1 vs Saliva_D91_Gp1Observed FeaturesShannon IndexSimpson Index
Comparison 6.Saliva_D0_Gp2 vs Saliva_D56_Gp2 vs Saliva_D70_Gp2 vs Saliva_D91_Gp2Observed FeaturesShannon IndexSimpson Index
Comparison 7.Feces_D0_Gp1 vs Feces_D0_Gp2Observed FeaturesShannon IndexSimpson Index
Comparison 8.Feces_D56_Gp1 vs Feces_D56_Gp2Observed FeaturesShannon IndexSimpson Index
Comparison 9.Feces_D70_Gp1 vs Feces_D70_Gp2Observed FeaturesShannon IndexSimpson Index
Comparison 10.Feces_D91_Gp1 vs Feces_D91_Gp2Observed FeaturesShannon IndexSimpson Index
Comparison 11.Feces_D0_Gp1 vs Feces_D56_Gp1 vs Feces_D70_Gp1 vs Feces_D91_Gp1Observed FeaturesShannon IndexSimpson Index
Comparison 12.Feces_D0_Gp2 vs Feces_D56_Gp2 vs Feces_D70_Gp2 vs Feces_D91_Gp2Observed FeaturesShannon IndexSimpson Index
Comparison 13.Plaque_D0_Gp1_Site12 vs Plaque_D0_Gp2_Site12Observed FeaturesShannon IndexSimpson Index
Comparison 14.Plaque_D56_Gp1_Site12 vs Plaque_D56_Gp2_Site12Observed FeaturesShannon IndexSimpson Index
Comparison 15.Plaque_D70_Gp1_Site12 vs Plaque_D70_Gp2_Site12Observed FeaturesShannon IndexSimpson Index
Comparison 16.Plaque_D91_Gp1_Site12 vs Plaque_D91_Gp2_Site12Observed FeaturesShannon IndexSimpson Index
Comparison 17.Plaque_D0_Gp1_Site12 vs Plaque_D56_Gp1_Site12 vs Plaque_D70_Gp1_Site12 vs Plaque_D91_Gp1_Site12Observed FeaturesShannon IndexSimpson Index
Comparison 18.Plaque_D0_Gp2_Site12 vs Plaque_D56_Gp2_Site12 vs Plaque_D70_Gp2_Site12 vs Plaque_D91_Gp2_Site12Observed FeaturesShannon IndexSimpson Index
Comparison 19.Plaque_D0_Gp1_Site34 vs Plaque_D0_Gp2_Site34Observed FeaturesShannon IndexSimpson Index
Comparison 20.Plaque_D56_Gp1_Site34 vs Plaque_D56_Gp2_Site34Observed FeaturesShannon IndexSimpson Index
Comparison 21.Plaque_D70_Gp1_Site34 vs Plaque_D70_Gp2_Site34Observed FeaturesShannon IndexSimpson Index
Comparison 22.Plaque_D91_Gp1_Site34 vs Plaque_D91_Gp2_Site34Observed FeaturesShannon IndexSimpson Index
Comparison 23.Plaque_D0_Gp1_Site34 vs Plaque_D56_Gp1_Site34 vs Plaque_D70_Gp1_Site34 vs Plaque_D91_Gp1_Site34Observed FeaturesShannon IndexSimpson Index
Comparison 24.Plaque_D0_Gp2_Site34 vs Plaque_D56_Gp2_Site34 vs Plaque_D70_Gp2_Site34 vs Plaque_D91_Gp2_Site34Observed FeaturesShannon IndexSimpson Index
 
 

IX. Analysis - Beta Diversity

 

NMDS and PCoA Plots

Beta diversity compares the similarity (or dissimilarity) of microbial profiles between different groups of samples. There are many different similarity/dissimilarity metrics [8]. In general, they can be quantitative (using sequence abundance, e.g., Bray-Curtis or weighted UniFrac) or binary (considering only presence-absence of sequences, e.g., binary Jaccard or unweighted UniFrac). They can be even based on phylogeny (e.g., UniFrac metrics) or not (non-UniFrac metrics, such as Bray-Curtis, etc.).

For microbiome studies, species profiles of samples can be compared with the Bray-Curtis dissimilarity, which is based on the count data type. The pair-wise Bray-Curtis dissimilarity matrix of all samples can then be subject to either multi-dimensional scaling (MDS, also known as PCoA) or non-metric MDS (NMDS).

MDS/PCoA is a scaling or ordination method that starts with a matrix of similarities or dissimilarities between a set of samples and aims to produce a low-dimensional graphical plot of the data in such a way that distances between points in the plot are close to original dissimilarities.

NMDS is similar to MDS, however it does not use the dissimilarities data, instead it converts them into the ranks and use these ranks in the calculation.

In our beta diversity analysis, Bray-Curtis dissimilarity matrix was first calculated and then plotted by the PCoA and NMDS separately. Below are beta diveristy results for all groups together:

References:

  1. Plantinga, AM, Wu, MC (2021). Beta Diversity and Distance-Based Analysis of Microbiome Data. In: Datta, S., Guha, S. (eds) Statistical Analysis of Microbiome Data. Frontiers in Probability and the Statistical Sciences. Springer, Cham. https://doi.org/10.1007/978-3-030-73351-3_5

 
 
NMDS and PCoA Plots for All Groups
 
 
 
 
 

The above PCoA and NMDS plots are based on count data. The count data can also be transformed into centered log ratio (CLR) for each species. The CLR data is no longer count data and cannot be used in Bray-Curtis dissimilarity calculation. Instead CLR can be compared with Euclidean distances. When CLR data are compared by Euclidean distance, the distance is also called Aitchison distance.

Below are the NMDS and PCoA plots of the Aitchison distances of the samples:

 
 
 
 
 
 
 
NMDS and PCoA Plots for Individual Comparisons at Species level
 
 
Comparison No.Comparison NameNMDAPCoA
Bray-CurtisCLR EuclideanBray-CurtisCLR Euclidean
Comparison 1Saliva_D0_Gp1 vs Saliva_D0_Gp2PDFSVGPDFSVGPDFSVGPDFSVG
Comparison 2Saliva_D56_Gp1 vs Saliva_D56_Gp2PDFSVGPDFSVGPDFSVGPDFSVG
Comparison 3Saliva_D70_Gp1 vs Saliva_D70_Gp2PDFSVGPDFSVGPDFSVGPDFSVG
Comparison 4Saliva_D91_Gp1 vs Saliva_D91_Gp2PDFSVGPDFSVGPDFSVGPDFSVG
Comparison 5Saliva_D0_Gp1 vs Saliva_D56_Gp1 vs Saliva_D70_Gp1 vs Saliva_D91_Gp1PDFSVGPDFSVGPDFSVGPDFSVG
Comparison 6Saliva_D0_Gp2 vs Saliva_D56_Gp2 vs Saliva_D70_Gp2 vs Saliva_D91_Gp2PDFSVGPDFSVGPDFSVGPDFSVG
Comparison 7Feces_D0_Gp1 vs Feces_D0_Gp2PDFSVGPDFSVGPDFSVGPDFSVG
Comparison 8Feces_D56_Gp1 vs Feces_D56_Gp2PDFSVGPDFSVGPDFSVGPDFSVG
Comparison 9Feces_D70_Gp1 vs Feces_D70_Gp2PDFSVGPDFSVGPDFSVGPDFSVG
Comparison 10Feces_D91_Gp1 vs Feces_D91_Gp2PDFSVGPDFSVGPDFSVGPDFSVG
Comparison 11Feces_D0_Gp1 vs Feces_D56_Gp1 vs Feces_D70_Gp1 vs Feces_D91_Gp1PDFSVGPDFSVGPDFSVGPDFSVG
Comparison 12Feces_D0_Gp2 vs Feces_D56_Gp2 vs Feces_D70_Gp2 vs Feces_D91_Gp2PDFSVGPDFSVGPDFSVGPDFSVG
Comparison 13Plaque_D0_Gp1_Site12 vs Plaque_D0_Gp2_Site12PDFSVGPDFSVGPDFSVGPDFSVG
Comparison 14Plaque_D56_Gp1_Site12 vs Plaque_D56_Gp2_Site12PDFSVGPDFSVGPDFSVGPDFSVG
Comparison 15Plaque_D70_Gp1_Site12 vs Plaque_D70_Gp2_Site12PDFSVGPDFSVGPDFSVGPDFSVG
Comparison 16Plaque_D91_Gp1_Site12 vs Plaque_D91_Gp2_Site12PDFSVGPDFSVGPDFSVGPDFSVG
Comparison 17Plaque_D0_Gp1_Site12 vs Plaque_D56_Gp1_Site12 vs Plaque_D70_Gp1_Site12 vs Plaque_D91_Gp1_Site12PDFSVGPDFSVGPDFSVGPDFSVG
Comparison 18Plaque_D0_Gp2_Site12 vs Plaque_D56_Gp2_Site12 vs Plaque_D70_Gp2_Site12 vs Plaque_D91_Gp2_Site12PDFSVGPDFSVGPDFSVGPDFSVG
Comparison 19Plaque_D0_Gp1_Site34 vs Plaque_D0_Gp2_Site34PDFSVGPDFSVGPDFSVGPDFSVG
Comparison 20Plaque_D56_Gp1_Site34 vs Plaque_D56_Gp2_Site34PDFSVGPDFSVGPDFSVGPDFSVG
Comparison 21Plaque_D70_Gp1_Site34 vs Plaque_D70_Gp2_Site34PDFSVGPDFSVGPDFSVGPDFSVG
Comparison 22Plaque_D91_Gp1_Site34 vs Plaque_D91_Gp2_Site34PDFSVGPDFSVGPDFSVGPDFSVG
Comparison 23Plaque_D0_Gp1_Site34 vs Plaque_D56_Gp1_Site34 vs Plaque_D70_Gp1_Site34 vs Plaque_D91_Gp1_Site34PDFSVGPDFSVGPDFSVGPDFSVG
Comparison 24Plaque_D0_Gp2_Site34 vs Plaque_D56_Gp2_Site34 vs Plaque_D70_Gp2_Site34 vs Plaque_D91_Gp2_Site34PDFSVGPDFSVGPDFSVGPDFSVG
 
 
 
 
 
 

Interactive 3D PCoA Plots - Bray-Curtis Dissimilarity

 
 
 

Interactive 3D PCoA Plots - Euclidean Distance

 
 
 

Interactive 3D PCoA Plots - Correlation Coefficients

 
 
 

Group Significance of Beta-diversity Indices

To test whether the between-group dissimilarities are significantly greater than the within-group dissimilarities, the "beta-group-significance" function provided in the QIIME 2 "diversity" package was used with PERMANOVA (permutational multivariate analysis of variance) as the group significant testing method.

Three beta diversity matrics were used: 1) Bray–Curtis dissimilarity 2) Correlation coefficient matrix , and 3) Aitchison distance (Euclidean distance between clr-transformed compositions).

 
 
Comparison 1.Saliva_D0_Gp1 vs Saliva_D0_Gp2Bray–CurtisCorrelationAitchison
Comparison 2.Saliva_D56_Gp1 vs Saliva_D56_Gp2Bray–CurtisCorrelationAitchison
Comparison 3.Saliva_D70_Gp1 vs Saliva_D70_Gp2Bray–CurtisCorrelationAitchison
Comparison 4.Saliva_D91_Gp1 vs Saliva_D91_Gp2Bray–CurtisCorrelationAitchison
Comparison 5.Saliva_D0_Gp1 vs Saliva_D56_Gp1 vs Saliva_D70_Gp1 vs Saliva_D91_Gp1Bray–CurtisCorrelationAitchison
Comparison 6.Saliva_D0_Gp2 vs Saliva_D56_Gp2 vs Saliva_D70_Gp2 vs Saliva_D91_Gp2Bray–CurtisCorrelationAitchison
Comparison 7.Feces_D0_Gp1 vs Feces_D0_Gp2Bray–CurtisCorrelationAitchison
Comparison 8.Feces_D56_Gp1 vs Feces_D56_Gp2Bray–CurtisCorrelationAitchison
Comparison 9.Feces_D70_Gp1 vs Feces_D70_Gp2Bray–CurtisCorrelationAitchison
Comparison 10.Feces_D91_Gp1 vs Feces_D91_Gp2Bray–CurtisCorrelationAitchison
Comparison 11.Feces_D0_Gp1 vs Feces_D56_Gp1 vs Feces_D70_Gp1 vs Feces_D91_Gp1Bray–CurtisCorrelationAitchison
Comparison 12.Feces_D0_Gp2 vs Feces_D56_Gp2 vs Feces_D70_Gp2 vs Feces_D91_Gp2Bray–CurtisCorrelationAitchison
Comparison 13.Plaque_D0_Gp1_Site12 vs Plaque_D0_Gp2_Site12Bray–CurtisCorrelationAitchison
Comparison 14.Plaque_D56_Gp1_Site12 vs Plaque_D56_Gp2_Site12Bray–CurtisCorrelationAitchison
Comparison 15.Plaque_D70_Gp1_Site12 vs Plaque_D70_Gp2_Site12Bray–CurtisCorrelationAitchison
Comparison 16.Plaque_D91_Gp1_Site12 vs Plaque_D91_Gp2_Site12Bray–CurtisCorrelationAitchison
Comparison 17.Plaque_D0_Gp1_Site12 vs Plaque_D56_Gp1_Site12 vs Plaque_D70_Gp1_Site12 vs Plaque_D91_Gp1_Site12Bray–CurtisCorrelationAitchison
Comparison 18.Plaque_D0_Gp2_Site12 vs Plaque_D56_Gp2_Site12 vs Plaque_D70_Gp2_Site12 vs Plaque_D91_Gp2_Site12Bray–CurtisCorrelationAitchison
Comparison 19.Plaque_D0_Gp1_Site34 vs Plaque_D0_Gp2_Site34Bray–CurtisCorrelationAitchison
Comparison 20.Plaque_D56_Gp1_Site34 vs Plaque_D56_Gp2_Site34Bray–CurtisCorrelationAitchison
Comparison 21.Plaque_D70_Gp1_Site34 vs Plaque_D70_Gp2_Site34Bray–CurtisCorrelationAitchison
Comparison 22.Plaque_D91_Gp1_Site34 vs Plaque_D91_Gp2_Site34Bray–CurtisCorrelationAitchison
Comparison 23.Plaque_D0_Gp1_Site34 vs Plaque_D56_Gp1_Site34 vs Plaque_D70_Gp1_Site34 vs Plaque_D91_Gp1_Site34Bray–CurtisCorrelationAitchison
Comparison 24.Plaque_D0_Gp2_Site34 vs Plaque_D56_Gp2_Site34 vs Plaque_D70_Gp2_Site34 vs Plaque_D91_Gp2_Site34Bray–CurtisCorrelationAitchison
 
 
 

X. Analysis - Differential Abundance

16S rRNA next generation sequencing (NGS) generates a fixed number of reads that reflect the proportion of different species in a sample, i.e., the relative abundance of species, instead of the absolute abundance. In Mathematics, measurements involving probabilities, proportions, percentages, and ppm can all be thought of as compositional data. This makes the microbiome read count data “compositional” (Gloor et al, 2017). In general, compositional data represent parts of a whole which only carry relative information [9].

The problem of microbiome data being compositional arises when comparing two groups of samples for identifying “differentially abundant” species. A species with the same absolute abundance between two conditions, its relative abundances in the two conditions (e.g., percent abundance) can become different if the relative abundance of other species change greatly. This problem can lead to incorrect conclusion in terms of differential abundance for microbial species in the samples.

When studying differential abundance (DA), the current better approach is to transform the read count data into log ratio data. The ratios are calculated between read counts of all species in a sample to a “reference” count (e.g., mean read count of the sample). The log ratio data allow the detection of DA species without being affected by percentage bias mentioned above

In this report, a compositional DA analysis tool “ANCOM” (analysis of composition of microbiomes) was used [10]. ANCOM transforms the count data into log-ratios and thus is more suitable for comparing the composition of microbiomes in two or more populations. "ANCOM" generates a table of features with W-statistics and whether the null hypothesis is rejected. The “W” is the W-statistic, or number of features that a single feature is tested to be significantly different against. Hence the higher the "W" the more statistical sifgnificant that a feature/species is differentially abundant.

References:

  1. Gloor GB, Macklaim JM, Pawlowsky-Glahn V, Egozcue JJ. Microbiome Datasets Are Compositional: And This Is Not Optional. Front Microbiol. 2017 Nov 15;8:2224. doi: 10.3389/fmicb.2017.02224. PMID: 29187837; PMCID: PMC5695134.
  2. Mandal S, Van Treuren W, White RA, Eggesbø M, Knight R, Peddada SD. Analysis of composition of microbiomes: a novel method for studying microbial composition. Microb Ecol Health Dis. 2015 May 29;26:27663. doi: 10.3402/mehd.v26.27663. PMID: 26028277; PMCID: PMC4450248.
 
 

ANCOM Differential Abundance Analysis

 
ANCOM Results for Individual Comparisons
Comparison No.Comparison Name
Comparison 1.Saliva_D0_Gp1 vs Saliva_D0_Gp2
Comparison 2.Saliva_D56_Gp1 vs Saliva_D56_Gp2
Comparison 3.Saliva_D70_Gp1 vs Saliva_D70_Gp2
Comparison 4.Saliva_D91_Gp1 vs Saliva_D91_Gp2
Comparison 5.Saliva_D0_Gp1 vs Saliva_D56_Gp1 vs Saliva_D70_Gp1 vs Saliva_D91_Gp1
Comparison 6.Saliva_D0_Gp2 vs Saliva_D56_Gp2 vs Saliva_D70_Gp2 vs Saliva_D91_Gp2
Comparison 7.Feces_D0_Gp1 vs Feces_D0_Gp2
Comparison 8.Feces_D56_Gp1 vs Feces_D56_Gp2
Comparison 9.Feces_D70_Gp1 vs Feces_D70_Gp2
Comparison 10.Feces_D91_Gp1 vs Feces_D91_Gp2
Comparison 11.Feces_D0_Gp1 vs Feces_D56_Gp1 vs Feces_D70_Gp1 vs Feces_D91_Gp1
Comparison 12.Feces_D0_Gp2 vs Feces_D56_Gp2 vs Feces_D70_Gp2 vs Feces_D91_Gp2
Comparison 13.Plaque_D0_Gp1_Site12 vs Plaque_D0_Gp2_Site12
Comparison 14.Plaque_D56_Gp1_Site12 vs Plaque_D56_Gp2_Site12
Comparison 15.Plaque_D70_Gp1_Site12 vs Plaque_D70_Gp2_Site12
Comparison 16.Plaque_D91_Gp1_Site12 vs Plaque_D91_Gp2_Site12
Comparison 17.Plaque_D0_Gp1_Site12 vs Plaque_D56_Gp1_Site12 vs Plaque_D70_Gp1_Site12 vs Plaque_D91_Gp1_Site12
Comparison 18.Plaque_D0_Gp2_Site12 vs Plaque_D56_Gp2_Site12 vs Plaque_D70_Gp2_Site12 vs Plaque_D91_Gp2_Site12
Comparison 19.Plaque_D0_Gp1_Site34 vs Plaque_D0_Gp2_Site34
Comparison 20.Plaque_D56_Gp1_Site34 vs Plaque_D56_Gp2_Site34
Comparison 21.Plaque_D70_Gp1_Site34 vs Plaque_D70_Gp2_Site34
Comparison 22.Plaque_D91_Gp1_Site34 vs Plaque_D91_Gp2_Site34
Comparison 23.Plaque_D0_Gp1_Site34 vs Plaque_D56_Gp1_Site34 vs Plaque_D70_Gp1_Site34 vs Plaque_D91_Gp1_Site34
Comparison 24.Plaque_D0_Gp2_Site34 vs Plaque_D56_Gp2_Site34 vs Plaque_D70_Gp2_Site34 vs Plaque_D91_Gp2_Site34
 
 

ANCOM-BC2 Differential Abundance Analysis

 

Starting with version V1.2, we include the results of ANCOM-BC (Analysis of Compositions of Microbiomes with Bias Correction) (Lin and Peddada 2020) [11]. ANCOM-BC is an updated version of "ANCOM" that:
(a) provides statistically valid test with appropriate p-values,
(b) provides confidence intervals for differential abundance of each taxon,
(c) controls the False Discovery Rate (FDR),
(d) maintains adequate power, and
(e) is computationally simple to implement.

The bias correction (BC) addresses a challenging problem of the bias introduced by differences in the sampling fractions across samples. This bias has been a major hurdle in performing DA analysis of microbiome data. ANCOM-BC estimates the unknown sampling fractions and corrects the bias induced by their differences among samples. The absolute abundance data are modeled using a linear regression framework.

Starting with version V1.43, ANCOM-BC2 is used instead of ANCOM-BC, So that multiple pairwise directional test can be performed (if there are more than two gorups in a comparison). When performing pairwise directional test, the mixed directional false discover rate (mdFDR) is taken into account. The mdFDR is the combination of false discovery rate due to multiple testing, multiple pairwise comparisons, and directional tests within each pairwise comparison. The mdFDR is adopted from (Guo, Sarkar, and Peddada 2010 [12]; Grandhi, Guo, and Peddada 2016 [13]). For more detail explanation and additional features of ANCOM-BC2 please see author's documentation.

References:

  1. Lin H, Peddada SD. Analysis of compositions of microbiomes with bias correction. Nat Commun. 2020 Jul 14;11(1):3514. doi: 10.1038/s41467-020-17041-7. PMID: 32665548; PMCID: PMC7360769.
  2. Guo W, Sarkar SK, Peddada SD. Controlling false discoveries in multidimensional directional decisions, with applications to gene expression data on ordered categories. Biometrics. 2010 Jun;66(2):485-92. doi: 10.1111/j.1541-0420.2009.01292.x. Epub 2009 Jul 23. PMID: 19645703; PMCID: PMC2895927.
  3. Grandhi A, Guo W, Peddada SD. A multiple testing procedure for multi-dimensional pairwise comparisons with application to gene expression studies. BMC Bioinformatics. 2016 Feb 25;17:104. doi: 10.1186/s12859-016-0937-5. PMID: 26917217; PMCID: PMC4768411.
 
 
ANCOM-BC Results for Individual Comparisons
 
Comparison No.Comparison Name
Comparison 1.Saliva_D0_Gp1 vs Saliva_D0_Gp2
Comparison 2.Saliva_D56_Gp1 vs Saliva_D56_Gp2
Comparison 3.Saliva_D70_Gp1 vs Saliva_D70_Gp2
Comparison 4.Saliva_D91_Gp1 vs Saliva_D91_Gp2
Comparison 5.Saliva_D0_Gp1 vs Saliva_D56_Gp1 vs Saliva_D70_Gp1 vs Saliva_D91_Gp1
Comparison 6.Saliva_D0_Gp2 vs Saliva_D56_Gp2 vs Saliva_D70_Gp2 vs Saliva_D91_Gp2
Comparison 7.Feces_D0_Gp1 vs Feces_D0_Gp2
Comparison 8.Feces_D56_Gp1 vs Feces_D56_Gp2
Comparison 9.Feces_D70_Gp1 vs Feces_D70_Gp2
Comparison 10.Feces_D91_Gp1 vs Feces_D91_Gp2
Comparison 11.Feces_D0_Gp1 vs Feces_D56_Gp1 vs Feces_D70_Gp1 vs Feces_D91_Gp1
Comparison 12.Feces_D0_Gp2 vs Feces_D56_Gp2 vs Feces_D70_Gp2 vs Feces_D91_Gp2
Comparison 13.Plaque_D0_Gp1_Site12 vs Plaque_D0_Gp2_Site12
Comparison 14.Plaque_D56_Gp1_Site12 vs Plaque_D56_Gp2_Site12
Comparison 15.Plaque_D70_Gp1_Site12 vs Plaque_D70_Gp2_Site12
Comparison 16.Plaque_D91_Gp1_Site12 vs Plaque_D91_Gp2_Site12
Comparison 17.Plaque_D0_Gp1_Site12 vs Plaque_D56_Gp1_Site12 vs Plaque_D70_Gp1_Site12 vs Plaque_D91_Gp1_Site12
Comparison 18.Plaque_D0_Gp2_Site12 vs Plaque_D56_Gp2_Site12 vs Plaque_D70_Gp2_Site12 vs Plaque_D91_Gp2_Site12
Comparison 19.Plaque_D0_Gp1_Site34 vs Plaque_D0_Gp2_Site34
Comparison 20.Plaque_D56_Gp1_Site34 vs Plaque_D56_Gp2_Site34
Comparison 21.Plaque_D70_Gp1_Site34 vs Plaque_D70_Gp2_Site34
Comparison 22.Plaque_D91_Gp1_Site34 vs Plaque_D91_Gp2_Site34
Comparison 23.Plaque_D0_Gp1_Site34 vs Plaque_D56_Gp1_Site34 vs Plaque_D70_Gp1_Site34 vs Plaque_D91_Gp1_Site34
Comparison 24.Plaque_D0_Gp2_Site34 vs Plaque_D56_Gp2_Site34 vs Plaque_D70_Gp2_Site34 vs Plaque_D91_Gp2_Site34
 
 
 
 
 

LEfSe - Linear Discriminant Analysis Effect Size

LEfSe (Linear Discriminant Analysis Effect Size) is an alternative method to find "organisms, genes, or pathways that consistently explain the differences between two or more microbial communities" (Segata et al., 2011) [14]. Specifically, LEfSe uses rank-based Kruskal-Wallis (KW) sum-rank test to detect features with significant differential (relative) abundance with respect to the class of interest. Since it is rank-based, instead of proportional based, the differential species identified among the comparison groups is less biased (than percent abundance based).

Reference:

  1. Segata N, Izard J, Waldron L, Gevers D, Miropolsky L, Garrett WS, Huttenhower C. Metagenomic biomarker discovery and explanation. Genome Biol. 2011 Jun 24;12(6):R60. doi: 10.1186/gb-2011-12-6-r60. PMID: 21702898; PMCID: PMC3218848.
 
Saliva_D0_Gp1 vs Saliva_D0_Gp2
 
 
 
 
 
 
 
LEfSe Results for All Comparisons
 
Comparison No.Comparison Name
Comparison 1.Saliva_D0_Gp1 vs Saliva_D0_Gp2
Comparison 2.Saliva_D56_Gp1 vs Saliva_D56_Gp2
Comparison 3.Saliva_D70_Gp1 vs Saliva_D70_Gp2
Comparison 4.Saliva_D91_Gp1 vs Saliva_D91_Gp2
Comparison 5.Saliva_D0_Gp1 vs Saliva_D56_Gp1 vs Saliva_D70_Gp1 vs Saliva_D91_Gp1
Comparison 6.Saliva_D0_Gp2 vs Saliva_D56_Gp2 vs Saliva_D70_Gp2 vs Saliva_D91_Gp2
Comparison 7.Feces_D0_Gp1 vs Feces_D0_Gp2
Comparison 8.Feces_D56_Gp1 vs Feces_D56_Gp2
Comparison 9.Feces_D70_Gp1 vs Feces_D70_Gp2
Comparison 10.Feces_D91_Gp1 vs Feces_D91_Gp2
Comparison 11.Feces_D0_Gp1 vs Feces_D56_Gp1 vs Feces_D70_Gp1 vs Feces_D91_Gp1
Comparison 12.Feces_D0_Gp2 vs Feces_D56_Gp2 vs Feces_D70_Gp2 vs Feces_D91_Gp2
Comparison 13.Plaque_D0_Gp1_Site12 vs Plaque_D0_Gp2_Site12
Comparison 14.Plaque_D56_Gp1_Site12 vs Plaque_D56_Gp2_Site12
Comparison 15.Plaque_D70_Gp1_Site12 vs Plaque_D70_Gp2_Site12
Comparison 16.Plaque_D91_Gp1_Site12 vs Plaque_D91_Gp2_Site12
Comparison 17.Plaque_D0_Gp1_Site12 vs Plaque_D56_Gp1_Site12 vs Plaque_D70_Gp1_Site12 vs Plaque_D91_Gp1_Site12
Comparison 18.Plaque_D0_Gp2_Site12 vs Plaque_D56_Gp2_Site12 vs Plaque_D70_Gp2_Site12 vs Plaque_D91_Gp2_Site12
Comparison 19.Plaque_D0_Gp1_Site34 vs Plaque_D0_Gp2_Site34
Comparison 20.Plaque_D56_Gp1_Site34 vs Plaque_D56_Gp2_Site34
Comparison 21.Plaque_D70_Gp1_Site34 vs Plaque_D70_Gp2_Site34
Comparison 22.Plaque_D91_Gp1_Site34 vs Plaque_D91_Gp2_Site34
Comparison 23.Plaque_D0_Gp1_Site34 vs Plaque_D56_Gp1_Site34 vs Plaque_D70_Gp1_Site34 vs Plaque_D91_Gp1_Site34
Comparison 24.Plaque_D0_Gp2_Site34 vs Plaque_D56_Gp2_Site34 vs Plaque_D70_Gp2_Site34 vs Plaque_D91_Gp2_Site34
 
 

XI. Analysis - Heatmap Profile

 

Species vs Sample Abundance Heatmap for All Samples

 
 
 

Heatmaps for Individual Comparisons

 
A) Two-way clustering - clustered on both columns (Samples) and rows (organism)
Comparison No.Comparison NameFamily LevelGenus LevelSpecies Level
Comparison 1Saliva_D0_Gp1 vs Saliva_D0_Gp2PDFSVGPDFSVGPDFSVG
Comparison 2Saliva_D56_Gp1 vs Saliva_D56_Gp2PDFSVGPDFSVGPDFSVG
Comparison 3Saliva_D70_Gp1 vs Saliva_D70_Gp2PDFSVGPDFSVGPDFSVG
Comparison 4Saliva_D91_Gp1 vs Saliva_D91_Gp2PDFSVGPDFSVGPDFSVG
Comparison 5Saliva_D0_Gp1 vs Saliva_D56_Gp1 vs Saliva_D70_Gp1 vs Saliva_D91_Gp1PDFSVGPDFSVGPDFSVG
Comparison 6Saliva_D0_Gp2 vs Saliva_D56_Gp2 vs Saliva_D70_Gp2 vs Saliva_D91_Gp2PDFSVGPDFSVGPDFSVG
Comparison 7Feces_D0_Gp1 vs Feces_D0_Gp2PDFSVGPDFSVGPDFSVG
Comparison 8Feces_D56_Gp1 vs Feces_D56_Gp2PDFSVGPDFSVGPDFSVG
Comparison 9Feces_D70_Gp1 vs Feces_D70_Gp2PDFSVGPDFSVGPDFSVG
Comparison 10Feces_D91_Gp1 vs Feces_D91_Gp2PDFSVGPDFSVGPDFSVG
Comparison 11Feces_D0_Gp1 vs Feces_D56_Gp1 vs Feces_D70_Gp1 vs Feces_D91_Gp1PDFSVGPDFSVGPDFSVG
Comparison 12Feces_D0_Gp2 vs Feces_D56_Gp2 vs Feces_D70_Gp2 vs Feces_D91_Gp2PDFSVGPDFSVGPDFSVG
Comparison 13Plaque_D0_Gp1_Site12 vs Plaque_D0_Gp2_Site12PDFSVGPDFSVGPDFSVG
Comparison 14Plaque_D56_Gp1_Site12 vs Plaque_D56_Gp2_Site12PDFSVGPDFSVGPDFSVG
Comparison 15Plaque_D70_Gp1_Site12 vs Plaque_D70_Gp2_Site12PDFSVGPDFSVGPDFSVG
Comparison 16Plaque_D91_Gp1_Site12 vs Plaque_D91_Gp2_Site12PDFSVGPDFSVGPDFSVG
Comparison 17Plaque_D0_Gp1_Site12 vs Plaque_D56_Gp1_Site12 vs Plaque_D70_Gp1_Site12 vs Plaque_D91_Gp1_Site12PDFSVGPDFSVGPDFSVG
Comparison 18Plaque_D0_Gp2_Site12 vs Plaque_D56_Gp2_Site12 vs Plaque_D70_Gp2_Site12 vs Plaque_D91_Gp2_Site12PDFSVGPDFSVGPDFSVG
Comparison 19Plaque_D0_Gp1_Site34 vs Plaque_D0_Gp2_Site34PDFSVGPDFSVGPDFSVG
Comparison 20Plaque_D56_Gp1_Site34 vs Plaque_D56_Gp2_Site34PDFSVGPDFSVGPDFSVG
Comparison 21Plaque_D70_Gp1_Site34 vs Plaque_D70_Gp2_Site34PDFSVGPDFSVGPDFSVG
Comparison 22Plaque_D91_Gp1_Site34 vs Plaque_D91_Gp2_Site34PDFSVGPDFSVGPDFSVG
Comparison 23Plaque_D0_Gp1_Site34 vs Plaque_D56_Gp1_Site34 vs Plaque_D70_Gp1_Site34 vs Plaque_D91_Gp1_Site34PDFSVGPDFSVGPDFSVG
Comparison 24Plaque_D0_Gp2_Site34 vs Plaque_D56_Gp2_Site34 vs Plaque_D70_Gp2_Site34 vs Plaque_D91_Gp2_Site34PDFSVGPDFSVGPDFSVG
 
 
B) One-way clustering - clustered on rows (organism) only
Comparison No.Comparison NameFamily LevelGenus LevelSpecies Level
Comparison 1Saliva_D0_Gp1 vs Saliva_D0_Gp2PDFSVGPDFSVGPDFSVG
Comparison 2Saliva_D56_Gp1 vs Saliva_D56_Gp2PDFSVGPDFSVGPDFSVG
Comparison 3Saliva_D70_Gp1 vs Saliva_D70_Gp2PDFSVGPDFSVGPDFSVG
Comparison 4Saliva_D91_Gp1 vs Saliva_D91_Gp2PDFSVGPDFSVGPDFSVG
Comparison 5Saliva_D0_Gp1 vs Saliva_D56_Gp1 vs Saliva_D70_Gp1 vs Saliva_D91_Gp1PDFSVGPDFSVGPDFSVG
Comparison 6Saliva_D0_Gp2 vs Saliva_D56_Gp2 vs Saliva_D70_Gp2 vs Saliva_D91_Gp2PDFSVGPDFSVGPDFSVG
Comparison 7Feces_D0_Gp1 vs Feces_D0_Gp2PDFSVGPDFSVGPDFSVG
Comparison 8Feces_D56_Gp1 vs Feces_D56_Gp2PDFSVGPDFSVGPDFSVG
Comparison 9Feces_D70_Gp1 vs Feces_D70_Gp2PDFSVGPDFSVGPDFSVG
Comparison 10Feces_D91_Gp1 vs Feces_D91_Gp2PDFSVGPDFSVGPDFSVG
Comparison 11Feces_D0_Gp1 vs Feces_D56_Gp1 vs Feces_D70_Gp1 vs Feces_D91_Gp1PDFSVGPDFSVGPDFSVG
Comparison 12Feces_D0_Gp2 vs Feces_D56_Gp2 vs Feces_D70_Gp2 vs Feces_D91_Gp2PDFSVGPDFSVGPDFSVG
Comparison 13Plaque_D0_Gp1_Site12 vs Plaque_D0_Gp2_Site12PDFSVGPDFSVGPDFSVG
Comparison 14Plaque_D56_Gp1_Site12 vs Plaque_D56_Gp2_Site12PDFSVGPDFSVGPDFSVG
Comparison 15Plaque_D70_Gp1_Site12 vs Plaque_D70_Gp2_Site12PDFSVGPDFSVGPDFSVG
Comparison 16Plaque_D91_Gp1_Site12 vs Plaque_D91_Gp2_Site12PDFSVGPDFSVGPDFSVG
Comparison 17Plaque_D0_Gp1_Site12 vs Plaque_D56_Gp1_Site12 vs Plaque_D70_Gp1_Site12 vs Plaque_D91_Gp1_Site12PDFSVGPDFSVGPDFSVG
Comparison 18Plaque_D0_Gp2_Site12 vs Plaque_D56_Gp2_Site12 vs Plaque_D70_Gp2_Site12 vs Plaque_D91_Gp2_Site12PDFSVGPDFSVGPDFSVG
Comparison 19Plaque_D0_Gp1_Site34 vs Plaque_D0_Gp2_Site34PDFSVGPDFSVGPDFSVG
Comparison 20Plaque_D56_Gp1_Site34 vs Plaque_D56_Gp2_Site34PDFSVGPDFSVGPDFSVG
Comparison 21Plaque_D70_Gp1_Site34 vs Plaque_D70_Gp2_Site34PDFSVGPDFSVGPDFSVG
Comparison 22Plaque_D91_Gp1_Site34 vs Plaque_D91_Gp2_Site34PDFSVGPDFSVGPDFSVG
Comparison 23Plaque_D0_Gp1_Site34 vs Plaque_D56_Gp1_Site34 vs Plaque_D70_Gp1_Site34 vs Plaque_D91_Gp1_Site34PDFSVGPDFSVGPDFSVG
Comparison 24Plaque_D0_Gp2_Site34 vs Plaque_D56_Gp2_Site34 vs Plaque_D70_Gp2_Site34 vs Plaque_D91_Gp2_Site34PDFSVGPDFSVGPDFSVG
 
 
C) No clustering
Comparison No.Comparison NameFamily LevelGenus LevelSpecies Level
Comparison 1Saliva_D0_Gp1 vs Saliva_D0_Gp2PDFSVGPDFSVGPDFSVG
Comparison 2Saliva_D56_Gp1 vs Saliva_D56_Gp2PDFSVGPDFSVGPDFSVG
Comparison 3Saliva_D70_Gp1 vs Saliva_D70_Gp2PDFSVGPDFSVGPDFSVG
Comparison 4Saliva_D91_Gp1 vs Saliva_D91_Gp2PDFSVGPDFSVGPDFSVG
Comparison 5Saliva_D0_Gp1 vs Saliva_D56_Gp1 vs Saliva_D70_Gp1 vs Saliva_D91_Gp1PDFSVGPDFSVGPDFSVG
Comparison 6Saliva_D0_Gp2 vs Saliva_D56_Gp2 vs Saliva_D70_Gp2 vs Saliva_D91_Gp2PDFSVGPDFSVGPDFSVG
Comparison 7Feces_D0_Gp1 vs Feces_D0_Gp2PDFSVGPDFSVGPDFSVG
Comparison 8Feces_D56_Gp1 vs Feces_D56_Gp2PDFSVGPDFSVGPDFSVG
Comparison 9Feces_D70_Gp1 vs Feces_D70_Gp2PDFSVGPDFSVGPDFSVG
Comparison 10Feces_D91_Gp1 vs Feces_D91_Gp2PDFSVGPDFSVGPDFSVG
Comparison 11Feces_D0_Gp1 vs Feces_D56_Gp1 vs Feces_D70_Gp1 vs Feces_D91_Gp1PDFSVGPDFSVGPDFSVG
Comparison 12Feces_D0_Gp2 vs Feces_D56_Gp2 vs Feces_D70_Gp2 vs Feces_D91_Gp2PDFSVGPDFSVGPDFSVG
Comparison 13Plaque_D0_Gp1_Site12 vs Plaque_D0_Gp2_Site12PDFSVGPDFSVGPDFSVG
Comparison 14Plaque_D56_Gp1_Site12 vs Plaque_D56_Gp2_Site12PDFSVGPDFSVGPDFSVG
Comparison 15Plaque_D70_Gp1_Site12 vs Plaque_D70_Gp2_Site12PDFSVGPDFSVGPDFSVG
Comparison 16Plaque_D91_Gp1_Site12 vs Plaque_D91_Gp2_Site12PDFSVGPDFSVGPDFSVG
Comparison 17Plaque_D0_Gp1_Site12 vs Plaque_D56_Gp1_Site12 vs Plaque_D70_Gp1_Site12 vs Plaque_D91_Gp1_Site12PDFSVGPDFSVGPDFSVG
Comparison 18Plaque_D0_Gp2_Site12 vs Plaque_D56_Gp2_Site12 vs Plaque_D70_Gp2_Site12 vs Plaque_D91_Gp2_Site12PDFSVGPDFSVGPDFSVG
Comparison 19Plaque_D0_Gp1_Site34 vs Plaque_D0_Gp2_Site34PDFSVGPDFSVGPDFSVG
Comparison 20Plaque_D56_Gp1_Site34 vs Plaque_D56_Gp2_Site34PDFSVGPDFSVGPDFSVG
Comparison 21Plaque_D70_Gp1_Site34 vs Plaque_D70_Gp2_Site34PDFSVGPDFSVGPDFSVG
Comparison 22Plaque_D91_Gp1_Site34 vs Plaque_D91_Gp2_Site34PDFSVGPDFSVGPDFSVG
Comparison 23Plaque_D0_Gp1_Site34 vs Plaque_D56_Gp1_Site34 vs Plaque_D70_Gp1_Site34 vs Plaque_D91_Gp1_Site34PDFSVGPDFSVGPDFSVG
Comparison 24Plaque_D0_Gp2_Site34 vs Plaque_D56_Gp2_Site34 vs Plaque_D70_Gp2_Site34 vs Plaque_D91_Gp2_Site34PDFSVGPDFSVGPDFSVG
 
 

XII. Analysis - Network Association

To analyze the co-occurrence or co-exclusion between microbial species among different samples, network correlation analysis tools are usually used for this purpose. However, microbiome count data are compositional. If count data are normalized to the total number of counts in the sample, the data become not independent and traditional statistical metrics (e.g., correlation) for the detection of specie-species relationships can lead to spurious results. In addition, sequencing-based studies typically measure hundreds of OTUs (species) on few samples; thus, inference of OTU-OTU association networks is severely under-powered. Here we use SPIEC-EASI (SParse InversE Covariance Estimation for Ecological Association Inference), a statistical method for the inference of microbial ecological networks from amplicon sequencing datasets that addresses both of these issues (Kurtz et al., 2015) [15]. SPIEC-EASI combines data transformations developed for compositional data analysis with a graphical model inference framework that assumes the underlying ecological association network is sparse. SPIEC-EASI provides two algorithms for network inferencing – 1) Meinshausen-Bühlmann's neighborhood selection (MB method) and inverse covariance selection (GLASSO method, i.e., graphical least absolute shrinkage and selection operator). This is fundamentally distinct from SparCC, which essentially estimate pairwise correlations. In addition to these two methods, we provide the results of a third method - SparCC (Sparse Correlations for Compositional Data)(Friedman & Alm 2012)[16], which is also a method for inferring correlations from compositional data. SparCC estimates the linear Pearson correlations between the log-transformed components.

References:

  1. Kurtz ZD, Müller CL, Miraldi ER, Littman DR, Blaser MJ, Bonneau RA. Sparse and compositionally robust inference of microbial ecological networks. PLoS Comput Biol. 2015 May 7;11(5):e1004226. doi: 10.1371/journal.pcbi.1004226. PMID: 25950956; PMCID: PMC4423992.
  2. Friedman J, Alm EJ. Inferring correlation networks from genomic survey data. PLoS Comput Biol. 2012;8(9):e1002687. doi: 10.1371/journal.pcbi.1002687. Epub 2012 Sep 20. PMID: 23028285; PMCID: PMC3447976.
 

SPIEC-EASI Network Inference by Neighborhood Selection (MB Method)

 
 

 

Association Network Inference by SparCC

 
 

 
 

XIII. Disclaimer

The results of this analysis are for research purpose only. They are not intended to diagnose, treat, cure, or prevent any disease. Forsyth and FOMC are not responsible for use of information provided in this report outside the research area.

 
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